LOCUS       sequence-263574-        4838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Drosophila Kinesin-1 truncated after neck-coil at 406.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Hancock
  TITLE     Hancock Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: K406
FEATURES             Location/Qualifiers
     source          1..4838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     33..52
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        33..51
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             83..105
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1331..1348
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1371..1388
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1441..1459)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1455..1502
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1539..1994
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1626..1645)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1836..1857
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2020..2124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2125..2193,2194..2985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2343..2362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3156..3744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3645..3664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3898..3915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3930..4069
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3995..4014)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4155..4177
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4171..4362)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 caacgctgcc cgagatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca
       61 acggtttccc tctagaaata attttgttta actttaagaa ggagatatac atatgagcgc
      121 agaacgagaa attccagccg aggacagcat caaagtggtc tgccgattcc gaccgctgaa
      181 cgacagcgaa gagaaggccg gctccaagtt cgtggtcaag ttccccaaca atgtggagga
      241 gaactgcata tccatagcgg gcaaggtgta tttgttcgac aaggtcttca aaccgaatgc
      301 atcccaggaa aaggtctaca atgaggcggc caagtccatt gttacggatg tcctggccgg
      361 gtacaatgga acgatattcg catacggtca gacgtcctcc ggaaaaacgc atacgatgga
      421 gggcgtgatc ggggactccg taaaacaggg tatcatacct aggatcgtca acgacatttt
      481 caatcacatc tacgcgatgg aggtgaacct ggagtttcac atcaaggtct cctactacga
      541 gatctacatg gacaagattc gagatctgtt ggacgtctcc aaggtgaacc ttagtgtgca
      601 cgaggataag aaccgggtgc cgtacgtcaa gggcgctacg gaacggttcg tctcgtcgcc
      661 ggaggatgtt ttcgaggtga tcgaggaggg caaatccaat cgtcacatcg ctgtgacaaa
      721 catgaacgag cattcttcgc gatcccactc agtattcctt atcaatgtga agcaggagaa
      781 cctggagaac cagaagaaac tatccggcaa actctacctg gtggatttgg ccggatccga
      841 gaaggtttcc aagactggag cggagggaac cgttcttgat gaagccaaga acatcaacaa
      901 gtcgctgtcg gccttgggca acgtaatttc tgccctggcg gacggaaaca aaacgcacat
      961 cccctaccgt gattccaagc taacgcgcat cctgcaggag tcgctgggag gcaacgcacg
     1021 cacaaccatc gtcatctgct gctctccagc cagtttcaac gaatctgaaa cgaagtcaac
     1081 gctggacttc ggtcgtagag ccaagacagt gaagaacgtg gtctgcgtta acgaggagct
     1141 tactgccgag gaatggaagc gacgctatga aaaggagaag gaaaagaacg cccgactaaa
     1201 gggtaaggtg gagaagcttg agatcgagct tgcgcgctgg agagcgggtg aaactgttaa
     1261 ggcggaggag caaatcaaca tggaggatct catggaggca agcacgccca acctggaagt
     1321 ggaggctagc catcatcatc atcatcacta agcggccgca ctcgatcgag caccaccacc
     1381 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     1441 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     1501 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
     1561 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1621 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1681 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1741 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1801 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     1861 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     1921 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     1981 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     2041 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2101 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     2161 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2221 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2281 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2341 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2401 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2461 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2521 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2581 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2641 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     2701 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2761 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2821 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2881 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2941 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     3001 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     3061 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3121 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3181 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3241 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3301 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3361 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3421 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3481 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3541 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3601 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3661 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3721 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3781 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3841 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3901 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     3961 tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4021 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     4081 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4141 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4201 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
     4261 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
     4321 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     4381 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
     4441 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
     4501 atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac
     4561 agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat
     4621 ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat
     4681 tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg
     4741 tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa
     4801 cgacaggagc acgatcatgc gcacccgtgg ccaggacc
//
