LOCUS       sequence-263585-        1685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescently tagged segment of DNA Helicase B that translocates 
            from the nucleus to the cytoplasm in response to phosphorylation by 
            CDK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Spencer SL, Cappell SD, Tsai FC, Overton KW, Wang CL, Meyer T
  TITLE     The proliferation-quiescence decision is controlled by a bifurcation
            in CDK2 activity at mitotic exit.
  JOURNAL   Cell. 2013 Oct 10;155(2):369-83. doi: 10.1016/j.cell.2013.08.062. 
            Epub 2013 Sep 26.
  PUBMED    24075009
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.08.062"; journalName: "Cell"; date: 
            "2013-10-10- 10"; volume: "155"; issue: "2"; pages: "369-83"
COMMENT     Sequence Label: DHB-mVenus
FEATURES             Location/Qualifiers
     source          1..1685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(327..329,330..1043)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(369..390)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(630..649)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     977..998
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1079..1667
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1132..1152)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1550..1561)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accggtctcg agaattcacc atgacaaatg atgtcacctg gagcgaggcc tcttcgcctg
       61 atgagaggac actcaccttt gctgaaagat ggcaattatc ttcacctgat ggagtagata
      121 cagatgatga tttaccaaaa tcgcgagcat ccaaaagaac ctgtggtgtg aatgatgatg
      181 aaagtccaag caaaattttt atggtgggag aatctccaca agtgtcttcc agacttcaga
      241 atttgagact gaataattta attcccaggc aacttttcaa gcccaccgat aatcaagaaa
      301 ctggatccgg ggcccagggc agcggcgtga gcaagggcga ggagctgttc accggggtgg
      361 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      421 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
      481 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg
      541 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      601 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      661 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      721 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
      781 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
      841 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      901 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca
      961 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     1021 gcatggacga gctgtacaag taatctagag ttaacatcga gggatcaagc ttatcgataa
     1081 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     1141 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     1201 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     1261 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     1321 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     1381 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     1441 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     1501 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     1561 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     1621 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     1681 agacc
//
