LOCUS       sequence-263588-       10033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene targeting in marmoset cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Yoshimatsu S, Okahara J, Sone T, Takeda Y, Nakamura M, Sasaki E, 
            Kishi N, Shiozawa S, Okano H
  TITLE     Robust and efficient knock-in in embryonic stem cells and 
            early-stage embryos of the common marmoset using the CRISPR-Cas9 
            system.
  JOURNAL   Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1038/s41598-018-37990-w.
  PUBMED    30728412
REFERENCE   2  (bases 1 to 10033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-6"; volume: "9(1):1528. doi"; pages: " 
            10.1038/s41598-018-37990-w"
COMMENT     Sequence Label: pKI-cjACTB-EGFP-3'short
FEATURES             Location/Qualifiers
     source          1..10033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(2764..2797)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2825..3398
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2848..3400
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2992..3009)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3219..3238
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      3418..3427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3424..3426,3427..3429,3430..4140)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3424..3426,3427..3429,3430..4140)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3469..3490)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3730..3749)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4077..4098
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4165..4230
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4237..5031
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    complement(5049..5082)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(6921..6940)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6922..6940)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6947..6964)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6947..6963)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6956..6978)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7101..7403
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7282..7301
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             7752..8546
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7752..8546
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      8746..8755
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8752..9126
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      9264..9852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9753..9772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10006..10023
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggtcttt
      301 gtccgagccg ggttcttgct cgggtgactg gagggtgggc gcggctgggc cactgccagg
      361 cccagtgggg gctgggctac cattgcgcgt gtgtgctggg gcttgtggcg ctgactgcgc
      421 gtgcgcgctg gagtctaggg cgccgccgct cgggctgggc ggagggcggg ccaggccgga
      481 aggggtgggg tcgccggggc gcaggcgcgc acttcctgcc tgcgccgccg gccgcctgag
      541 ggtgtggccg ctttgcacgc tagtgcgcgc cgaaaccggc gttttttgaa ccgggcggag
      601 gcggggccag cccctggtgg ggagggggcg gaggcctggc ttcctgccgc gcgccgcggg
      661 gccgcctccg accagtgttt gccttttatg gtaataacgc ggccggcccg gcttcctttg
      721 tccccaatcc gggcgcgcgc cggcgccccc tggcggccca aggccgaggc gcgccggaag
      781 tgagtagggc gggggcgacc ccggctcaca gagcgccctg ctcttcccgc agctcaccat
      841 ggatgatgat atcgctgcac ttgttgtcga caacggctcc ggcatgtgca aggccggctt
      901 tgcgggcgac gatgctcccc gggccgtctt tccctctatc gtggggcgcc ccaggcacca
      961 ggtaaggaag ttgagtgggt ggggccgccc caggagcggg cgggaggcga gggcacttag
     1021 gaggtacccc ggcttccggg gtgggggtcg cgcccgtgtt caaagcaccc tgttctttgc
     1081 agggcgtgat ggtgggcatg ggtcagaagg actcctacgt gggtgacgag gcccagagca
     1141 agagaggcat cctgaccctg aagtacccca tcgagcatgg cattgtcacc aactgggacg
     1201 acatggagaa gatctggcac cacacttttt acaacgagct gcgtgtggct cctgaggagc
     1261 accccgtgct gcttactgag gcccccctga accccaaggc caaccgtgag aagatgaccc
     1321 aggtgagtga tctgccacct cccctggtgg ccgcctccct ccttcctggc ctcccggagc
     1381 tgcgcccttt ctcactggtt ctctcttctg ccgttctccg taggactctc ttctctgacc
     1441 tgagtctcct ttggaactct gcaggttcta tttgcttttt cccagatgag gtctttctgg
     1501 tgtttgcctt tctgactagg tgtctaaaac agacagtgct gtgggtgtag gtactaacac
     1561 tggctcgtgt gacaagccca tgaggctggt gtaaagcagt cttggagtgt gtatacagta
     1621 ggcgcacagt aggtctgaac agaatcccca tcccaagacc ccagcacaac tagcggtgtt
     1681 cttgcactta ctgcatgtcc cctgtctgct cttggctgtc cctagtgact tcctcagtgt
     1741 gacacggtgc atctctttct acagatcatg tttgagacct tcaacacccc agccatgtat
     1801 gtggccatcc aggctgtgct ttccttgtac gcctctggcc gtaccactgg catcgtgatg
     1861 gactccggtg acggggtcac ccacactgtg cccatctatg aggggtatgc cctcccccac
     1921 gccatcctac gtctggacct ggctggccgg gacctgactg actacctcat gaagatcctc
     1981 accgagcgcg gctacagctt caccaccaca gctgagcggg aaatcgtgcg tgacatcaag
     2041 gagaagctgt gctacgtcgc cctggacttc gagcaggaga tggccacggc ggcctccagc
     2101 tcctccctgg agaagagcta tgagctgccc gatggtcagg tcatcaccat cggcaacgag
     2161 cggttccgct gccctgaggc actcttccag ccttctttcc tgggtaagtg gagaccactg
     2221 cctagccctg cctgacatga ggttaccttg gggctgtgct gtggaagctt aagtctgggt
     2281 ctcatttccc cctcaggcat ggaatcttgt ggcatccacg aaactacctt taactccatc
     2341 atgaaatgtg acgtggacat ccgcaaagac ctgtatgcca acacagtgct gtctggtggt
     2401 accaccatgt accctggcat cgctgacagg atgcagaagg agatcaccgc cctggcaccc
     2461 agcacaatga agatcaaggt gggtgtcctt ccagcctgag ctgacctggg cagggggcat
     2521 ggggccctgt ggtttgtggg gagctgtcat atcaagggtc ctcactgcct gttccctcca
     2581 ctcctcagat cattgctcct cccgagcgca agtactcagt gtggattggt ggctccatcc
     2641 tggcctcgct gtccaccttc cagcagatgt ggatcagcaa gcaggagtat gatgagtccg
     2701 gcccctccat cgtccaccgc aaatgcttct aggcggactg tgacttagtg aattcgccct
     2761 tggataactt cgtatagcat acattatacg aagttataga tctccctttt ttttccacag
     2821 tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     2881 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     2941 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     3001 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     3061 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     3121 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     3181 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     3241 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     3301 ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     3361 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacgga tctgctagcc
     3421 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3481 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3541 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3601 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     3661 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3721 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3781 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3841 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     3901 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     3961 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     4021 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     4081 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     4141 aggcctcgcg ctaagcgggc accggtgaaa cagactttga attttgacct tctcaagttg
     4201 gcgggagacg tggagtccaa cccagggccg gatatcatga ttgaacaaga tggattgcac
     4261 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     4321 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     4381 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     4441 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     4501 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     4561 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     4621 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     4681 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     4741 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg atgatctcgt cgtgacccat
     4801 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     4861 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     4921 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     4981 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcggccggc
     5041 cgctcgacat aacttcgtat aggatacttt atacgaagtt atgatatcca tcacactggc
     5101 ggccgcgggc ttacctgtac actgacttga gaccagttga ataaagtgca caccttaaaa
     5161 atgaggccaa gcatgacttt gtgtcgggct ggggtgtggg cagcaggtga acccagggga
     5221 agggtggggc agtgggggcc agcctgtccc tactgagtgg aggtgggctg aggttctctc
     5281 tccttgacat tgagcagcct tagaatgtga gggaagttta agattcaggc ctctgctcat
     5341 tggggagttc ctggcttggc cctgccacct gtccctaggt accccagttt ctctgggttc
     5401 atccagaagg tctagatgct caggaagtgc tggttggcac ctgtgtttct taggagtgga
     5461 gatcccttag ggtcactccg ggcagggggt tcttgagtgg gagtgtagtg ggtgttccta
     5521 gctgccatgc ctttgtcctg ctcactgtat tggagtggag tcgtccatag gctgaggaga
     5581 agccatgact cctgcttctc tacccagaca gggaaatctg ggatcacagt cgaattgtgg
     5641 tgggggatcc atggggccat gtggttggca ggcagctctg gttagctctg gactgagctg
     5701 gattctgcct tgtgacgagt aggcgccatc ctgtggcctc tcagggccca gtttgtgacc
     5761 catgctgcag agggagatgg ccagactcct ggacagcagg gcatgcctgg cattgcaggt
     5821 cagattcttt tttttttgag atggggtctg gctttgttgc tcaggcttta gttcagtggt
     5881 acaaccacag ctcactgcag cctcagcctt ctgggcaagc agttctgcct cagcctccca
     5941 aatagctgga actacacagg cacacccagc tagttgtggg tcagattctc acttgtgcag
     6001 agctgagtca cttttgaaag aaacaaaacc tggcttccct tccgctgcta gaagctttga
     6061 ttgatagtag cggagaatga aggaaggaga cgaggctccc ttgcggggat accctttacc
     6121 aaaaacacca cctggtgtat cacagttgtt ctggaaatga tctggtcccc aggaggcctg
     6181 gactgcctct cacttgtgca cagtggctgc tggcagagag gaaggttgtc cctttctgtg
     6241 ttatcttgga ggttccccga agggtctgcc aagagcagtt ctagctcttt tggtctttac
     6301 tgagtgacca ctagatggtg ggcctgaagc tactgagatg acctggacaa gaaccctgcc
     6361 ttggaggagc tgtcaatctg gaaatgagga cttcattcat ttgttcattc cttgatccat
     6421 ttctccattg aatcactatt tgtgctatgt actgtgggta tggaggagaa ccaaacagat
     6481 gtgttcctgt cctcaccagg cacccaattt aaaggggaag acatcataaa cacaaaggta
     6541 cacaaatgag tgtccaatga cacggtgtca tcatgggaag aaaaagcaga ggctttgatg
     6601 aacacgatca ttaggtgata ttcattatgt tgattgtggt ggtctagaca taaattaaca
     6661 cttatctagg ctgggcgtgg tggctcatgc ctgtaatccc agcactttgg gaggctgagg
     6721 tgggtggatc aagagatcga gaccatcctg gccaacatag tgaaacccca tctttactaa
     6781 aaatacaaaa attagctggg cgtggtggtg cacacttggg aggctgaggt aggagaactg
     6841 cttgaactgg ggaggcgaac gttgcagtga gctaagattg tgctactgta ctccagcctg
     6901 gtgctagagg gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
     6961 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     7021 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     7081 tgcgcagcct atacgtacgg cagtttaagg tttacaccta taaaagagag agccgttatc
     7141 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc ggggcgacgg atggtgatcc
     7201 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     7261 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     7321 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     7381 acctgatgtt ctggggaata taaatgtcag gcatgagatt atcaaaaagg atcttcacct
     7441 agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca
     7501 gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca
     7561 gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat
     7621 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     7681 tcttgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag
     7741 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     7801 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     7861 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     7921 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     7981 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     8041 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     8101 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     8161 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     8221 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     8281 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     8341 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     8401 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     8461 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     8521 atcgccttct tgacgagttc ttctgaatta ttaacgctta caatttcctg atgcggtatt
     8581 ttctccttac gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat
     8641 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8701 agacaataac cctgataaat gcttcaataa tagcacgtga ggagggccac catggccaag
     8761 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     8821 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     8881 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     8941 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     9001 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     9061 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     9121 gactgacacg tgctaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9181 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9481 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9541 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9601 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9661 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9721 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9781 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9841 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9901 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9961 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10021 gaggaagcgg aag
//
