LOCUS       sequence-263606-        2529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2020-04-14"; volume: "13(627). pii: 
            13/627/eaba4200. doi"; pages: " 10.1126/scisignal.aba4200"
COMMENT     Sequence Label: pBABE_hygro_mRFP1_NRF2(C506S, L4A)
FEATURES             Location/Qualifiers
     source          1..2529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..675
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             1..675
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(131..149)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     399..418
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     593..616
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             691..714
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc
       61 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      121 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      181 ctgtcccctc agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      241 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      301 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      361 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      421 atgggctggg aggcctccac cgagcggatg taccccgagg acggcgccct gaagggcgag
      481 atcaagatga ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc
      541 tacatggcca agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac
      601 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc
      661 cactccaccg gcgccctcga gggatccatg gactacaagg atgacgatga caagatggac
      721 ttggagctgc cgccgccggg actcccgtcc cagcaggaca tggatttgat tgacatactt
      781 tggaggcaag atatagatct tggagtaagt cgagaagtat ttgacttcag tcagcgacgg
      841 aaagagtatg agctggaaaa acagaaaaaa cttgaaaagg aaagacaaga acaactccaa
      901 aaggagcaag agaaagcctt tttcgctcag ttacaactag atgaagagac aggtgaattt
      961 ctcccaattc agccagccca gcacatccag tcagaaacca gtggatctgc caactactcc
     1021 caggttgccc acattcccaa atcagatgct ttgtactttg atgactgcat gcagcttttg
     1081 gcgcagacat tcccgtttgt agatgacaat gaggtttctt cggctacgtt tcagtcactt
     1141 gttcctgata ttcccggtca catcgagagc ccagtcttca ttgctactaa tcaggctcag
     1201 tcacctgaaa cttctgttgc tcaggtagcc cctgttgatt tagacggtat gcaacaggac
     1261 attgagcaag tttgggagga gctattatcc attcctgagt tacagtgtct taatattgaa
     1321 aatgacaagc tggttgagac taccatggtt ccaagtccag aagccaaact gacagaagtt
     1381 gacaattatc atttttactc atctataccc tcaatggaaa aagaagtagg taactgtagt
     1441 ccacattttc ttaatgcttt tgaggattcc ttcagcagca tcctctccac agaagacccc
     1501 aaccagttga cagtgaactc attaaattca gatgccacag tcaacacaga ttttggtgat
     1561 gaattttatt ctgctttcat agctgagccc agtatcagca acagcatgcc ctcacctgct
     1621 actttaagcc attcactctc tgaacttcta aatgggccca ttgatgtttc tgatctatca
     1681 ctttgcaaag ctttcaacca aaaccaccct gaaagcacag cagaattcaa tgattctgac
     1741 tccggcattt cactaaacac aagtcccagt gtggcatcac cagaacactc agtggaatct
     1801 tccagctatg gagacacact acttggcctc agtgattctg aagtggaaga gctagatagt
     1861 gcccctggaa gtgtcaaaca gaatggtcct aaaacaccag tacattcttc tggggatatg
     1921 gtacaaccct tgtcaccatc tcaggggcag agcactcacg tgcatgatgc ccaatgtgag
     1981 aacacaccag agaaagaatt gcctgtaagt cctggtcatc ggaaaacccc attcacaaaa
     2041 gacaaacatt caagccgctt ggaggctcat ctcacaagag atgaacttag ggcaaaagct
     2101 ctccatatcc cattccctgt agaaaaaatc attaacctcc ctgttgttga cttcaacgaa
     2161 atgatgtcca aagagcagtt caatgaagct caacttgcat taattcggga tatacgtagg
     2221 aggggtaaga ataaagtggc tgctcagaat agcagaaaaa gaaaactgga aaatatagta
     2281 gaactagagc aagatttaga tcatttgaaa gatgaaaaag aaaaattgct caaagaaaaa
     2341 ggagaaaatg acaaaagcct tcacctagcg aaaaaacaag ccagcaccgc atatgccgaa
     2401 gttttcagca tgctacgtga tgaagatgga aaaccttatt ctcctagtga atactccctg
     2461 cagcaaacaa gagatggcaa tgttttcctt gttcccaaaa gtaagaagcc agatgttaag
     2521 aaaaactag
//
