LOCUS       sequence-263611-        8111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Schmelzle T, Mailleux AA, Overholtzer M, Carroll JS, Solimini NL, 
            Lightcap ES, Veiby OP, Brugge JS
  TITLE     Functional role and oncogene-regulated expression of the BH3-only 
            factor Bmf in mammary epithelial anoikis and morphogenesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Mar 6. 104(10):3787-92.
  PUBMED    17360431
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Mar 6. 104(10):3787-92."
COMMENT     Sequence Label: pLNCX myr Akt1
FEATURES             Location/Qualifiers
     source          1..8111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1512..2306
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1566..1585)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2176..2195
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     enhancer        2788..3167
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3168..3371
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3322..3342
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3368..3392
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3471..3491
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     LTR             5013..5606
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5709..5731)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5817..5957
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5872..5891
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5972..5989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6143..6731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6223..6242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6902..7693,7694..7762))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7525..7544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7763..7867)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7935..7953
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aattccgatc tgatcaagag acaggatgag
     1501 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     1561 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     1621 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     1681 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     1741 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     1801 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     1861 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     1921 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     1981 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     2041 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     2101 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     2161 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     2221 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     2281 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     2341 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     2401 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     2461 ggagttcttc gcccaccccg ggctcgatcc cctcgcgagt tggttcagct gctgcctgag
     2521 gctggacgac ctcgcggagt tctaccggca gtgcaaatcc gtcggcatcc aggaaaccag
     2581 cagcggctat ccgcgcatcc atgcccccga actgcaggag tggggaggca cgatggccgc
     2641 tttggtcgag gcggatccgg ccattagcca tattattcat tggttatata gcataaatca
     2701 atattggcta ttggccattg catacgttgt atccatatca taatatgtac atttatattg
     2761 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
     2821 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     2881 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     2941 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3001 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3061 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3121 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3181 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3241 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3301 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3361 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     3421 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccaagctt atggggagca
     3481 gcaagagcaa gcccaagtct agaatgaacg acgtagccat tgtgaaggag ggctggctgc
     3541 acaaacgagg ggaatatatt aaaacctggc ggccacgcta cttcctcctc aagaacgatg
     3601 gcacctttat tggctacaag gaacggcctc aggatgtgga tcagcgagag tccccactca
     3661 acaacttctc agtggcacaa tgccagctga tgaagacaga gcggccaagg cccaacacct
     3721 ttatcatccg ctgcctgcag tggaccacag tcattgagcg caccttccat gtggaaacgc
     3781 ctgaggagcg ggaagaatgg gccaccgcca ttcagactgt ggccgatgga ctcaagaggc
     3841 aggaagaaga gacgatggac ttccgatcag gctcacccag tgacaactca ggggctgaag
     3901 agatggaggt gtccctggcc aagcccaagc accgtgtgac catgaacgag tttgagtacc
     3961 tgaaactact gggcaagggc acctttggga aagtgattct ggtgaaagag aaggccacag
     4021 gccgctacta tgccatgaag atcctcaaga aggaggtcat cgtcgccaag gatgaggttg
     4081 cccacacgct tactgagaac cgtgtcctgc agaactctag gcatcccttc cttacggccc
     4141 tcaagtactc attccagacc cacgaccgcc tctgctttgt catggagtat gccaacgggg
     4201 gcgagctctt cttccacctg tctcgagagc gcgtgttctc cgaggaccgg gcccgcttct
     4261 atggtgcgga gattgtgtct gccctggact acttgcactc cgagaagaac gtggtgtacc
     4321 gggacctgaa gctggagaac ctcatgctgg acaaggacgg gcacatcaag ataacggact
     4381 tcgggctgtg caaggagggg atcaaggatg gtgccactat gaagacattc tgcggaacgc
     4441 cggagtacct ggcccctgag gtgctggagg acaacgacta cggccgtgca gtggactggt
     4501 gggggctggg cgtggtcatg tatgagatga tgtgtggccg cctgcccttc tacaaccagg
     4561 accacgagaa gctgttcgag ctgatcctca tggaggagat ccgcttcccg cgcacactcg
     4621 gccctgaggc caagtccctg ctctccgggc tgctcaagaa ggaccctaca cagaggctcg
     4681 gtgggggctc tgaggatgcc aaggagatca tgcagcaccg gttctttgcc aacatcgtgt
     4741 ggcaggatgt gtatgagaag aagctgagcc cacctttcaa gccccaggtc acctctgaga
     4801 ctgacaccag gtatttcgat gaggagttca cagctcagat gatcaccatc acgccgcctg
     4861 atcaagatga cagcatggag tgtgtggaca gtgagcggag gccgcacttc ccccagttct
     4921 cctactcagc cagtggcaca gcctgaggcg gatccctaac acgagccata gataaaataa
     4981 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     5041 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag
     5101 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
     5161 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
     5221 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     5281 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     5341 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     5401 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     5461 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     5521 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     5581 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc
     5641 agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac
     5701 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     5761 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     5821 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     5881 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     5941 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6001 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6061 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6121 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6181 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6241 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6301 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6361 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6481 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6541 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     6601 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6661 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6721 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6781 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6841 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6901 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6961 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7021 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7081 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7141 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7201 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7261 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7321 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7381 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7441 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7501 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7561 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7621 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7681 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7741 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7801 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7861 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     7921 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttcataccag
     7981 atcaccgaaa actgtcctcc aaatgtgtcc ccctcacact cccaaattcg cgggcttctg
     8041 cctcttagac cactctaccc tattccccac actcaccgga gccaaagccg cggcccttcc
     8101 gtttctttgc t
//
