LOCUS       sequence-263618-       11600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis,
            C. orthopsilosis, and C. metapsilosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11600)
  AUTHORS   Lombardi L, Oliveira-Pacheco J, Butler G
  TITLE     Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species.
  JOURNAL   mSphere. 2019 Mar 13;4(2). pii: 4/2/e00125-19. doi: 
            10.1128/mSphere.00125-19.
  PUBMED    30867327
REFERENCE   2  (bases 1 to 11600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2019-03-13"; volume: "4(2). pii: 4/2/e00125-19. doi"; pages: 
            " 10.1128/mSphere.00125-19"
COMMENT     Sequence Label: pCP-tRNA
FEATURES             Location/Qualifiers
     source          1..11600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(542..564)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     664..683
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     877..899
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     891..908
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     892..908
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        1643..1718
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           1723..1790
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             complement(1933..1950)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2601..6704
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2601..6704
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6717..6737
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6965..6979)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             7633..7653
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9556..9573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9727..10315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9807..9826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10486..11277,11278..11346))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11109..11128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11347..11451)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atatttaatt cattattttg agggttattg caacaatcta cggaggtgtt gaagggcgcc
       61 attctatgaa gttcaggaaa aagaaaatgt tgctggttga tgtgtatcaa gggatgagca
      121 tgaacgctcg tttaaaagga tattgttatt gatccaaggg gccattgttt cccacaaacg
      181 tgctcaacat tactacctga ttgccgtaaa actgatccct tccggaaagc caattgaatt
      241 attttgtgtt tgatcataaa ttgacgcaaa tttgaaacaa atttttgaat ttatgataac
      301 cacttaatcg actgctctta gagactgtaa ctcaagaatc caaccacaaa ccctcatgga
      361 ctcctttcgg tgcctaacca agagaatatt cccacagatt agcgaagcgt gctagaatcg
      421 cccggagacg gtcacagctt gtctgtaact aagaaaccat tattatcatg acattaacct
      481 ataaaaatag gcgtatcacg aggccctttc atctcgcgcg tttcggtgat gacggtgaaa
      541 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
      601 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
      661 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
      721 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
      781 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
      841 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
      901 cggccagtga attggagatc ggtacttcgg tactttggtg taactggggt attttgcccg
      961 gtgataaaat agaagggacg tgggaatagt tgtgagtgga aattgttgat ccgatatgtt
     1021 gaggaaaagt ccgatgtgaa tcgaatgata gaggtattac tttaaataag gaaaagggtg
     1081 tcggttgggt tgtgagtttg tgcgtctaag cattgtcagt cgtccgtcat catttgcaga
     1141 ccgaacccca ttgagctagg gtcgtagaac cacgtaggta gggtgagatt agcagtgctg
     1201 tatatccgat ggacgattat gacccggcgg tatgactttg ttgcagcgcg agtgtgcact
     1261 attgtacaaa aagcaaaatt aaaaatgaat atatgctacc atatgatttt taactacata
     1321 cgttctactc tcttccgtat tacaaacaaa caactgacac aaaagaagac aacaactaat
     1381 gagaaaaatg tcacgttgac ccgaaatctt tttctccttt ctttgccatt ttctgattat
     1441 ttaaccaatt gaaatttctc aacaaatttt taaatacaat tcttcttcct tccttccttc
     1501 cttctttctt tctttctttt ccaaattcaa aacaaagagc ttaaaatggg cgtgtggcgt
     1561 agttggtagc gcgttccctt agcatgggaa aggtcatgag ttcgattctt atctcgtcca
     1621 ggaagagctc gcgagctctt ccgttttaga gctagaaata gcaagttaaa ataaggctag
     1681 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttggccggca tggtcccagc
     1741 ctcctcgctg gcgccggctg ggcaacatgc ttcggcatgg cgaatgggac ctatccgtga
     1801 agttataaga aggcaattaa gagctagcta gctagtcttt ggttttcttt tggttttata
     1861 tatttttcgt atatgagttt ataaataaag cgaatgcgtc gagataccgg tacaccatca
     1921 ttgatgatga tcatgatgat ggtggtggtg tcagattgcc ctccccgaga aagtctaggc
     1981 caaaacagaa atatggcaaa ttaatgggaa gcgtgtcgcc actgtcaatg agaagtaagc
     2041 ttacggatgc atccatgaca tcaagatcaa ccattattat ggacgatgaa gatgacgatg
     2101 aaagagaagg tactgatgcg gtaggggctg ggttaaattg tgatgtcaca aacgatgaag
     2161 acttgtcttc gcaagttagg tacgaggacg ccgagtcaat gatgaatcaa gaacgtcttt
     2221 tgaagaaatt ggaagaggag tcacctcaat atagacggga aacgggattg actgcaaatg
     2281 gtagctcaac aaaacggcgt aagactagag ggcagtagcc ctattactac agaccacgtg
     2341 acctttatca gatctgttaa cttcaatttt ttgagaacaa tttggcatat ctaggatgtt
     2401 taaaatatat agcgaaccaa ttaaagacca tcccgctgac cactacactt gcaagacgat
     2461 gctaatagca aatttgaaac tagaaaggtg gtgtgctttg accaattggt agaacttaca
     2521 tcttcttaca acgaattcta cggtgctcta ctagtgcatc ctattgttgc atcatagact
     2581 tagagagtga ccggtccggt atggacaaga agtactctat cggtttggac atcggtacta
     2641 actctgttgg ttgggctgtt atcactgacg aatacaaggt tccatctaag aagttcaagg
     2701 ttttgggtaa cactgacaga cactctatca agaagaactt gatcggtgct ttgttgttcg
     2761 actctggtga aactgctgaa gctactagat tgaagagaac tgctagaaga agatacacta
     2821 gaagaaagaa cagaatctgt tacttgcaag aaatcttctc taacgaaatg gctaaggttg
     2881 acgactcttt cttccacaga ttggaagaat ctttcttggt tgaagaagac aagaagcacg
     2941 aaagacaccc aatcttcggt aacatcgttg acgaagttgc ttaccacgaa aagtacccaa
     3001 ctatctacca cttgagaaag aagttggttg actctactga caaggctgac ttgagattga
     3061 tctacttggc tttggctcac atgatcaagt tcagaggtca cttcttgatc gaaggtgact
     3121 tgaacccaga caactctgac gttgacaagt tgttcatcca attggttcaa acttacaacc
     3181 aattgttcga agaaaaccca atcaacgctt ctggtgttga cgctaaggct atcttgtctg
     3241 ctagattgtc taagtctaga agattggaaa acttgatcgc tcaattgcca ggtgaaaaga
     3301 agaacggttt gttcggtaac ttgatcgctt tgtctttggg tttgactcca aacttcaagt
     3361 ctaacttcga cttggctgaa gacgctaagt tgcaattgtc taaggacact tacgacgacg
     3421 acttggacaa cttgttggct caaatcggtg accaatacgc tgacttgttc ttggctgcta
     3481 agaacttgtc tgacgctatc ttgttgtctg acatcttgag agttaacact gaaatcacta
     3541 aggctccatt gtctgcttct atgatcaaga gatacgacga acaccaccaa gacttgactt
     3601 tgttgaaggc tttggttaga caacaattgc cagaaaagta caaggaaatc ttcttcgacc
     3661 aatctaagaa cggttacgct ggttacatcg acggtggtgc ttctcaagaa gaattctaca
     3721 agttcatcaa gccaatcttg gaaaagatgg acggtactga agaattgttg gttaagttga
     3781 acagagaaga cttgttgaga aagcaaagaa ctttcgacaa cggttctatc ccacaccaaa
     3841 tccacttggg tgaattgcac gctatcttga gaagacaaga agacttctac ccattcttga
     3901 aggacaacag agaaaagatc gaaaagatct tgactttcag aatcccatac tacgttggtc
     3961 cattggctag aggtaactct agattcgctt ggatgactag aaagtctgaa gaaactatca
     4021 ctccatggaa cttcgaagaa gttgttgaca agggtgcttc tgctcaatct ttcatcgaaa
     4081 gaatgactaa cttcgacaag aacttgccaa acgaaaaggt tttgccaaag cactctttgt
     4141 tgtacgaata cttcactgtt tacaacgaat tgactaaggt taagtacgtt actgaaggta
     4201 tgagaaagcc agctttcttg tctggtgaac aaaagaaggc tatcgttgac ttgttgttca
     4261 agactaacag aaaggttact gttaagcaat tgaaggaaga ctacttcaag aagatcgaat
     4321 gtttcgactc tgttgaaatc tctggtgttg aagacagatt caacgcttct ttgggtactt
     4381 accacgactt gttgaagatc atcaaggaca aggacttctt ggacaacgaa gaaaacgaag
     4441 acatcttgga agacatcgtt ttgactttga ctttgttcga agacagagaa atgatcgaag
     4501 aaagattgaa gacttacgct cacttgttcg acgacaaggt tatgaagcaa ttgaagagaa
     4561 gaagatacac tggttggggt agattgtcta gaaagttgat caacggtatc agagacaagc
     4621 aatctggtaa gactatcttg gacttcttga agtctgacgg tttcgctaac agaaacttca
     4681 tgcaattgat ccacgacgac tctttgactt tcaaggaaga catccaaaag gctcaagttt
     4741 ctggtcaagg tgactctttg cacgaacaca tcgctaactt ggctggttct ccagctatca
     4801 agaagggtat cttgcaaact gttaaggttg ttgacgaatt ggttaaggtt atgggtagac
     4861 acaagccaga aaacatcgtt atcgaaatgg ctagagaaaa ccaaactact caaaagggtc
     4921 aaaagaactc tagagaaaga atgaagagaa tcgaagaagg tatcaaggaa ttgggttctc
     4981 aaatcttgaa ggaacaccca gttgaaaaca ctcaattgca aaacgaaaag ttgtacttgt
     5041 actacttgca aaacggtaga gacatgtacg ttgaccaaga attggacatc aacagattgt
     5101 ctgactacga cgttgaccac atcgttccac aatctttctt gaaggacgac tctatcgaca
     5161 acaaggtttt gactagatct gacaagaaca gaggtaagtc tgacaacgtt ccatctgaag
     5221 aagttgttaa gaagatgaag aactactgga gacaattgtt gaacgctaag ttgatcactc
     5281 aaagaaagtt cgacaacttg actaaggctg aaagaggtgg tttgtctgaa ttggacaagg
     5341 ctggtttcat caagagacaa ttggttgaaa ctagacaaat cactaagcac gttgctcaaa
     5401 tcttggactc tagaatgaac actaagtacg acgaaaacga caagttgatc agagaagtta
     5461 aggttatcac tttgaagtct aagttggttt ctgacttcag aaaggacttc caattctaca
     5521 aggttagaga aatcaacaac taccaccacg ctcacgacgc ttacttgaac gctgttgttg
     5581 gtactgcttt gatcaagaag tacccaaagt tggaatctga attcgtttac ggtgactaca
     5641 aggtttacga cgttagaaag atgatcgcta agtctgaaca agaaatcggt aaggctactg
     5701 ctaagtactt cttctactct aacatcatga acttcttcaa gactgaaatc actttggcta
     5761 acggtgaaat cagaaagaga ccattgatcg aaactaacgg tgaaactggt gaaatcgttt
     5821 gggacaaggg tagagacttc gctactgtta gaaaggtttt gtctatgcca caagttaaca
     5881 tcgttaagaa gactgaagtt caaactggtg gtttctctaa ggaatctatc ttgccaaaga
     5941 gaaactctga caagttgatc gctagaaaga aggactggga cccaaagaag tacggtggtt
     6001 tcgactctcc aactgttgct tactctgttt tggttgttgc taaggttgaa aagggtaagt
     6061 ctaagaagtt gaagtctgtt aaggaattgt tgggtatcac tatcatggaa agatcttctt
     6121 tcgaaaagaa cccaatcgac ttcttggaag ctaagggtta caaggaagtt aagaaggact
     6181 tgatcatcaa gttgccaaag tactctttgt tcgaattgga aaacggtaga aagagaatgt
     6241 tggcttctgc tggtgaattg caaaagggta acgaattggc tttgccatct aagtacgtta
     6301 acttcttgta cttggcttct cactacgaaa agttgaaggg ttctccagaa gacaacgaac
     6361 aaaagcaatt gttcgttgaa caacacaagc actacttgga cgaaatcatc gaacaaatct
     6421 ctgaattctc taagagagtt atcttggctg acgctaactt ggacaaggtt ttgtctgctt
     6481 acaacaagca cagagacaag ccaatcagag aacaagctga aaacatcatc cacttgttca
     6541 ctttgactaa cttgggtgct ccagctgctt tcaagtactt cgacactact atcgacagaa
     6601 agagatacac ttctactaag gaagttttgg acgctacttt gatccaccaa tctatcactg
     6661 gtttgtacga aactagaatc gacttgtctc aattgggtgg tgactctaaa gctgacccaa
     6721 agaagaagag aaaggtttaa atcggatcca ttatgtaggt gaatggtgat ttgtttttga
     6781 acaacttgtt gtttaaaatc ctgttcaaca cagaatcaaa ctgtatattc tgattaagtt
     6841 caggagcata atgatgatag tcattcaatg cctttttgac ggctttggtt gtcgtcagat
     6901 tgccattttt ataaatgttt tcttggagtt ctgcagcaga catccactct ttagtggttg
     6961 cagttttatc atcatcatca gaatcagatt caatttcctg caacaacata aacccatcac
     7021 cagcacaaca acatcccaat aaatcatggg tgtttgactt ggtatccacg tcaatttcaa
     7081 acaagttttt caagtcgcta taatcaaaca catctgattt cgatgaagca tcgttgtcaa
     7141 gaaactttga actcaatttg ttcttcatca actgccgttg gaatatcttc tcatcgatac
     7201 aaccagtagt gaaaagcctg tatatataac acggcttcat ttggccatct cgatgaatcc
     7261 tcgacattga ttgcaaatct gtagatggat tccaatcatt atcgtatagg attagccttg
     7321 acgcaccaac caaattgata ccagtcccac ccgatttcga ggatagtaaa aacaccagaa
     7381 tcagttcgtc cttgttgaac ttgttgacga gcttgcctct catgttgttt ggtgttgatc
     7441 catccaagcg caagtattgt agattgagct ttttgatcac agtctccaac aagtccaaag
     7501 tagtggtgta gtttgaaatg agcactaatt tctctttttg tgctgtaatc tccaacacca
     7561 atggtatgag tgcatttagt ttacctgagc tagtagataa tttcaagctt ccatcggtta
     7621 taggatcctg acccaaagaa gaagagaaag gtttaaggga tcccgggccc gtcgactgca
     7681 gaggcctgca tgcgtcaaaa ctagagaata ataaagaaaa cgatcttttc aaaaagaaaa
     7741 aaccttttag ttttcctttg ttgttgttgt gggtgtgtgc tatttatatt atatagttta
     7801 ctcataatac cataaaatat tcggtttgat taggttattt taataagcta atttgtttct
     7861 aatcgtgtaa tttatgctgt gtatattagg tagtgtgtgc actgcccaaa aatgtttgtt
     7921 gtttatagtc ggttaaagag aaaaaagaaa aaaagatcca tacacacacg ttaattagtt
     7981 gttcaacgta atacactcat attttgttct tatttgcttt cggtcgctgt tctcaccaag
     8041 atttattgcc aacgaaacaa ttttttttta tatattttca gatttttctt tttttccttt
     8101 cctttccttt tctaattttc actcctggtt ttctttcttt cttagaaaca ttatctcgat
     8161 attaatatta aaaaaatata atcattcaaa atggacggtg gtatgtttta gtttagcttc
     8221 aattctaatt gattgattaa tcagtttatt ggtttcaata tgacaaatgg gtagggtggg
     8281 aaaacttcat tttcaattca gatcaaactt ttttgttgtc gacataatat ttctcgtttg
     8341 ggatgttact gtcacattaa taatacacac acatcagctt ataattttga aagtaattta
     8401 tcagatatgt tgtgacgatc agtggaaatg gctaacttca atgtatctgt tcttcccctt
     8461 tttcaaagtt cacgattttt gattgattga ttgatctgtc ggcagtggtt tcaaaaccat
     8521 tcggtgagta atcctaccaa tcaatgttac gacaaaaggc tcaatattca aaattgcaat
     8581 gttttatgtt ttcctacgtg tacttgtgca aggcaattga ttcaacattg cttttggtgt
     8641 ttgacgagtt tctagtttgg acttgtgttg ttatctgggc tatacagatt tcccggctca
     8701 ctatgaattt tttttttcga cgctcagtgc acacaactat aaacaacaca aacacaaaca
     8761 cagcaagaaa aaaaaaaaac gaacattgaa ttgaaaccaa gccaactgaa aaattcctta
     8821 tttaaatgac tgtcatacta acccattttt atagaagaag ttgctgcttt agttatcgat
     8881 aacggttctc atatgaaaat ttcggtgatc cctgagcagg tggcggaaac attggatgct
     8941 gagaaccatt tcattgttcg tgaagtgttc gatgtgcacc tatccgacca aggctttgaa
     9001 ctatctacca gaagtgtgag cccctaccgg aaggattaca tctcggatga tgactctgat
     9061 gaagactctg cttgctatgg cgcattcatc gaccaagagc ttgtcgggaa gattgaactc
     9121 aactcaacat ggaacgatct agcctctatc gaacacattg ttgtgtcgca cacgcaccga
     9181 ggcaaaggag tcgcgcacag tctcatcgaa tttgcgaaaa agtgggcact aagcagacag
     9241 ctccttggca tacgattaga gacacaaacg aacaatgtac ctgcctgcaa tttgtacgca
     9301 aaatgtggct ttactctcgg cggcattgac ctcttcacgt ataaaactag acctcaagtc
     9361 tcgaacgaaa cagcgatgta ctggtactgg ttctcgggag cacaggatga cgcctaacat
     9421 atgtgaagtg tgaaggggga gattttcact ttattagatt tgtatatatg tataataaat
     9481 aaataaataa gttaaataaa taattagata agggtggtaa ttattactat ttacaatcaa
     9541 aggtggtcct gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9601 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9661 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9721 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9781 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9841 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9901 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9961 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    10021 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    10081 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    10141 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    10201 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    10261 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10381 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10441 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10501 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10561 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10621 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10681 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10741 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10801 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10861 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10921 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10981 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    11041 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    11101 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    11161 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    11221 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    11281 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    11341 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11401 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11461 ccgaaaagtg ccacctgacg tataggcgta tcacgaggcc ctttcgtctc gcgcgatacc
    11521 tcttactgat atttcttgcg ttgtgatcga gcttatggat gaggcaatca ccgggccaat
    11581 ctactacgag agaccgcgat
//
