LOCUS       sequence-263628-        6189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses KLF3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Himeda CL, Ranish JA, Pearson RC, Crossley M, Hauschka SD
  TITLE     KLF3 regulates muscle-specific gene expression and synergizes with 
            serum response factor on KLF binding sites.
  JOURNAL   Mol Cell Biol. 2010 Jul;30(14):3430-43. doi: 10.1128/MCB.00302-10. 
            Epub 2010 Apr 19.
  PUBMED    20404088
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00302-10"; journalName: "Mol Cell Biol"; date: 
            "2010-07"; volume: "30"; issue: "14"; pages: "3430-43"
COMMENT     Sequence Label: pMT3-FLAG-KLF3
FEATURES             Location/Qualifiers
     source          1..6189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          446..675
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     658..677
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      701..710
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             710..733
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1885..2448
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    2635..2769
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2672..2691)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2726..2745
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3442..3459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3613..4201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3693..3712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4372..5163,5164..5232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4995..5014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5233..5337)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5405..5423
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5461..5483)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5583..5602
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5796..5818
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5810..5827
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5811..5827
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5837..6166)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5851..5986
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6151..6171
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aatttcgagc ggtgttccgc ggtcctcctc gtatagaaac tcggaccact ctgagacgaa
       61 ggctcgcgtc caggccagca cgaaggaggc taagtgggag gggtagcggt cgttgtccac
      121 tagggggtcc actcgctcca gggtgtgaag acacatgtcg ccctcttcgg catcaaggaa
      181 ggtgattggt ttataggtgt aggccacgtg accgggtgtt cctgaagggg ggctataaaa
      241 gggggtgggg gcgcgttcgt cctcactctc ttccgcatcg ctgtctgcga gggccagctg
      301 ttgggctcgc ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac
      361 ccgtcggcct ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg
      421 atcggaaaac ctctcgactg ttggggtgag tactccctct caaaagcggg catgacttct
      481 gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg
      541 atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc
      601 ttgaggtgtg gcaggcttga gatctggcca tacacttgag tgacaatgac atccactttg
      661 cctttctctc cacaggtgtc cactcccagg tccaactgca gccaccatgg attacaagga
      721 tgacgacgat aagctcgaca ccatgctcat gtttgatcca gtccctgtca agcaggaggc
      781 catggaccct gtctcggtgt ccttcccgtc gaattacata gaatcgatga agcccaacaa
      841 atatggggtc atttactcca caccgttgcc tgataagttc ttccagaccc cagaaggcct
      901 cactcacggg atacaggtgg agccggtgga cctcacggtg aacaagcggg gctccccgcc
      961 tgctgcaggc ggttctcctt cctctctgaa gttcccgtcc caccggagag cgtcacctgg
     1021 cctcagcatg ccctcctcca gtccgcccat taagaagtac tcgccccctt ctcctggcgt
     1081 gcagcccttt ggagtacccc tgtctatgcc gcctgtgatg gcagccgcgc tgtccagaca
     1141 cggaatccgg agcccaggca tcctccccgt cattcagccc gtcgtggtcc agcccgttcc
     1201 ttttatgtat accagccacc tgcagcagcc tctcatggtc tccttgtcgg aagagatgga
     1261 caattcaaac agcggcatgc cagtacctgt aattgaatca tatgagaagc ccttattaca
     1321 gaaaaaaatt aaaatagagc ctggaattga accacagagg actgactatt accctgaaga
     1381 aatgtcaccc cctttaatga acccagtgtc ccccccgcaa gccttgttgc aagagaacca
     1441 tccttccgtc atcgtgcagc ctgggaagag acctctacct gtggaatccc cggataccca
     1501 aaggaagcgc aggatacaca gatgcgacta tgatgggtgc aataaggtct acactaagag
     1561 ctcgcacttg aaagcacaca gaagaactca tacaggagaa aagccgtaca aatgcacctg
     1621 ggaaggctgc acgtggaagt tcgctcgatc tgatgaactg acgagacatt tccgaaaaca
     1681 cactgggatc aaacctttcc agtgtccaga ctgtgaccga agcttctctc gctctgacca
     1741 ccttgcccta cacaggaaac gccacatgct agtctgagaa ttccgggggg gggggggggg
     1801 ggacagctca gggctgcgat ttcgcgccaa acttgacggc aatcctagcg tgaaggctgg
     1861 taggatttta tccccgctgc catcatggtt cgaccattga actgcatcgt cgccgtgtcc
     1921 caaaatatgg ggattggcaa gaacggagac ctaccctggc ctccgctcag gaacgagttc
     1981 aagtacttcc aaagaatgac cacaacctct tcagtggaag gtaaacagaa tctggtgatt
     2041 atgggtagga aaacctggtt ctccattcct gagaagaatc gacctttaaa ggacagaatt
     2101 aatatagttc tcagtagaga actcaaagaa ccaccacgag gagctcattt tcttgccaaa
     2161 agtttggatg atgccttaag acttattgaa caaccggaat tggcaagtaa agtagacatg
     2221 gtttggatag tcggaggcag ttctgtttac caggaagcca tgaatcaacc aggccacctc
     2281 agactctttg tgacaaggat catgcaggaa tttgaaagtg acacgttttt cccagaaatt
     2341 gatttgggga aatataaact tctcccagaa tacccaggcg tcctctctga ggtccaggag
     2401 gaaaaaggca tcaagtataa gtttgaagtc tacgagaaga aagactaaca ggaagatgct
     2461 ttcaagttct ctgctcccct cctaaagcta tgcattttta taagaccatg ggacttttgc
     2521 tggctttaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     2581 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     2641 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2701 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     2761 gtctggatcc ccggccaacg gtctggtgac ccggctgcga gagctcggtg tacctgagac
     2821 gcgagtaagc ccttgagtca aagacgtagt cgttgcaagt ccgcaccagg tactgatatc
     2881 ccaccaaaaa gtgcggcggc ggctggcggt agaggggcca gcgtagggtg gccggggctc
     2941 cgggggcgag gtcttccaac ataaggcgat gatatccgta gatgtacctg gacatccagg
     3001 tgatgccggc ggcggtggtg gaggcgcgcg gaaagtcgcg gacgcggttc cagatgttgc
     3061 gcagcggcaa aaagtgctcc atggtcggga cgctctggcc ggtgaggcgt gcgcagtcgt
     3121 tgacgctcta gaccgtgcaa aaggagagcc tgtaagcggg cactcttccg tggtctggtg
     3181 gataaattcg caagggtatc atggcggacg accggggttc gaaccccgga tccggccgtc
     3241 cgccgtgatc catgcggtta ccgcccgcgt gtcgaaccca ggtgtgcgac gtcagacaac
     3301 gggggagcgc tccttttggc ttccttccag gcgcggcggc tgctgcgcta gcttttttgg
     3361 cgagctcgaa ttaattctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3421 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3481 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3781 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4021 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4681 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4981 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5341 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     5401 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     5461 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     5521 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     5581 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     5641 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     5701 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     5761 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     5821 ggccagtgcc aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     5881 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     5941 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     6001 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     6061 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     6121 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacaggatcc
     6181 ggtcgcgcg
//
