LOCUS       sequence-263644-       12425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of eGFP tagged PCNA to monitor DNA replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 12425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0620
FEATURES             Location/Qualifiers
     source          1..12425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1640..2434
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      complement(2926..3381)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3063..3084)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3275..3294
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3507..3529
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3521..3538
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3522..3538
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3567
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3548..3566
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3591..3607
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3592..3608
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(6119..6121,6122..6124,6125..6835)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6119..6121,6122..6124,6125..6835)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6164..6185)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6425..6444)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6772..6793
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     gene            8984..10103
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        8984..9327
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             9328..9900
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      9906..10103
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(10165..10185)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10165..10183)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10204..10220)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10204..10220)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10217..10239)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10228..10244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10252..10258,10259..10276,10277..10282))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10297..10318
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10435..10452)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10606..11194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10686..10705)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11365..12156,12157..12225))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11988..12007
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12226..12330)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12398..12416
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcgctt acataatgag tgagtgtcat tattgaggaa tgccatgtca
      721 gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt tttgcccaag
      781 gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc actcttttta
      841 gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg cgtattggat
      901 atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat gagcaaactg
      961 cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat cgcatggaag
     1021 atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa attttgattc
     1081 catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg ctatatgtat
     1141 gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc tttctctaaa
     1201 taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt atttgtttgt
     1261 atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc atggtgaaaa
     1321 agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag ccttaatgcc
     1381 cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt gaatatattt
     1441 tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt ttgtctttat
     1501 aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat ctctgcacat
     1561 gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg atcgaaatta
     1621 aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag ctagagctga
     1681 ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa agcaaagcaa
     1741 cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg tagataaaat
     1801 tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt tcgaccagga
     1861 tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct ttgaggatcg
     1921 caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg tgtacaaaat
     1981 tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta ttatacaagg
     2041 cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg aaatgtcttc
     2101 caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt ttgagaagca
     2161 taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc aaagcgacta
     2221 cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg gacagcaata
     2281 tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg ttggtcgtgg
     2341 agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag aagctggttg
     2401 gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa aatttttttg
     2461 gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg ttttatgtca
     2521 gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta gcgactaaaa
     2581 tattaactat tatagataaa caccttggga ataaaaagta atttgctata gtaatttatt
     2641 aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg aataagaaaa
     2701 gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa agatgtatgt
     2761 agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa acattggtgt
     2821 tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt gctagggcgg
     2881 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     2941 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3001 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3061 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3121 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3181 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3241 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3301 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3361 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3421 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3481 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3541 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga
     3601 cggtatcgat aagcttgata tcgaattcct gcagcccaac tgcggtcaag atatttcttg
     3661 aatcaggcgc cttagaccgc tcggccaaac aaccaattac ttgttgagaa atagagtata
     3721 attatcctat aaatataacg tttttgaaca cacatgaaca aggaagtaca ggacaattga
     3781 ttttgaagag aatgtggatt ttgatgtaat tgttgggatt ccatttttaa taaggcaata
     3841 atattaggta tgtagatata ctagaagttc tcctcgacaa gcttgcatgc agaactggat
     3901 tatttttcgt tcttctagaa agtacgcgaa ttgtacgacg aatttcatct tcacgtccga
     3961 taactggatc cagttgacca tttctagcga gctctgtaag atcaacagta aatttattta
     4021 gtgcatcaaa tccttcctcg gcattctttg aatcgattct tttattacca cggacgttat
     4081 ttacagcaaa ttcaaacgct ttcggggtga caccggcttc tgccaataaa ctctttagcg
     4141 tgtcgtcttt agtgaacaca gcgataaaat gatcttgagc aatgtaagaa tctttttgtg
     4201 ttttttgaag ttcatgagca tttcgcagca gtttcgcact ttcaggtgac agagtcactt
     4261 gttctggagg tggatcctga gcaggcaagc gaactaaccg actagtaaca cttcgctcaa
     4321 acttttgacc atcaccacca gctttgtcga cgatggtgcg cagtaaggta gtaccgttac
     4381 tgtcactgtc ggacaaaaga gcagcagcaa tgtgaatagg ggttaattgt gaatgaccat
     4441 aagattgtgc aattgagtac gcatcagaca atgtctttgc ggctttgtca gtaaaaggat
     4501 aatcagccat atcgagttat attattatag gaatttttat aatttataga taaatccaaa
     4561 aaaaaggaat tgtatatttg tcgctcgtta taaaacaaaa aagagagagt cgttgaacgg
     4621 taaatatata cggttttcag ttcgatttaa taagaactta atatcgttat tataataaat
     4681 tcttcggtta tagattgatg gttgaggatg ccgcaggtag agaagctgtg catatatata
     4741 cacgatgatg ttactatggg aaggaaagga acataccaga agatgccagt tacattgtta
     4801 ctcgaaccta ctcctacact ctagaaaaag aaagggataa agtagggtaa atcccaaaga
     4861 catgactaga agatttaaaa aaataagaga aaaatagctt aaaaaaaaac cggtgtaaat
     4921 taaaaatgca gttaccttaa aagtctttga attgtcttta aacatggaat actcgaaacc
     4981 cggaaatgat ctgcgtatta cgtagtgtat ggaacaacgt aaagacggat actaacctca
     5041 acttattctc aattgcatct tgctaaatac cgttagaatt catcaatgac tgaataaata
     5101 attttttgat acatagttct tctattacag agattctagt catatttcca agctatttct
     5161 aagatagagc tcttacatta cttattctat acggtagcga ttattcgtcg taatgacatt
     5221 taacgaactt tagttacttg accatagtgg tttataaact aaacaaacca acagtgaatt
     5281 cttgttaacg taactgtaat ctacgaatat tgagaagaaa aaaaaacgaa tgaatttgtt
     5341 gtgaatacat attttttcct tagcgaacaa gtatacttca ttcttatttt catacctctt
     5401 tagtcttatt taaaccagca ataaaattcg ttttaagttt tccgtgttaa taccaattaa
     5461 aataacgttt ttttcaatca ttttatatgt cattgcaacg tgaaaataag ttaataaatt
     5521 tcggtagcag ctaatatgga aaatgtacaa tttacttgat acattagtgt agcctaaatt
     5581 ttatttgatc ctggaaagta tattaaggta gaacgcgaaa gctctcctag tttacgccgt
     5641 aaattgtaat gactcgctga ttacatttgg cttattataa tccgtatagc tagaaaattt
     5701 cgataactga atattgcttc tttaaaaaga cataaataga accgtgaatt agtacagcaa
     5761 acgaaagata aaaatttcat agtaacatgc aaatactatt ttttcagtta gttttccata
     5821 attttgtata atatttcata tcaaacgcat ctaaaaagta cggacgaaat tacttgctat
     5881 tgtaaaaata aaaaaaataa caaaacaaat tataataacg tgaactgcta ttttggtctc
     5941 atatatgaaa ggttcttgag attttttacg ttgtcagcta gcaattttac gcacactatt
     6001 ctgtgctacg cgctctttga ctacttccca aaagccaact acaaaaggtt tttgcagtaa
     6061 cctttgttag ctattcttta tatctcttat ctagcttgta taaaatataa ttacgaccat
     6121 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     6181 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     6241 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     6301 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     6361 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     6421 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     6481 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     6541 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     6601 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     6661 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     6721 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     6781 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg
     6841 actcagatcg ggtgagggtc aaggtcaagg tcaaggtcct ggtcgtggtt acgcttaccg
     6901 tagtagatct atgcttgaag ctagatttca gcaggctgct ttgttgaaaa agcttctcga
     6961 tgccatcaag gagttagtta cagatgctaa ttttgattgc aatgacaacg gtatctctct
     7021 gcaggctatg gatagttctc atgtagccct tgtttctatg cttattaaat cagatggatt
     7081 tgaaccatat cggtgtgata gaaatattgc tttaggtata aatctgaacg ccttatcaaa
     7141 ggtcttacgg tgtgcccaaa acgaggattt ggttacttta aaggctgaag atacacctga
     7201 ggtattgaat ttggttttcg agagcgagaa aaatgacagg atctctgatt atgatgttaa
     7261 attaatggac attgatcaag aacacttggg tataccagat atcgaatacg atgctactat
     7321 tactatgcct gctgccgaat ttcaacgcat tactcgagat ttattaactt tgagtgattc
     7381 agtaacaatc aatgctagta aagagggtgt tcgttttagc tgcaagggag atattggtaa
     7441 tggtagtacc acgctgaaac aacataccga tctttctgac caagatcaaa gtatcgaaat
     7501 ttctcttacc caagctgtaa cacttacgtt ttctttgaaa tatttagccc agttcactaa
     7561 ggctactcct ttagctaccc gagtcacact ttcaatgagc aatgatgttc cacttcttgt
     7621 ggagtataaa atggaatccg gatttttacg attttatctt gccccaaaaa ttggtgagga
     7681 ggatgaggag tagattacgg aatcaatatc ttttagtaat ctctttgcaa ttgacgagct
     7741 agcatcatta taaacatact tttgtttact ttttaattca accttttttg tactgctcct
     7801 tccctaacat tcatatccaa acaggcaaaa taccagcttt tacttaaaaa aaatcaaatt
     7861 ctttagaacc aacaaactga agggtagaaa aacttgtgtt cttagtgatg atttttcaca
     7921 gataacttcg taatcggatc cccgggtaaa aggaatgtct cccttgccag tactgctagg
     7981 gtttttcttt caaactatgg aagcccattc aagctgcata ttacgatttt gtttttcgct
     8041 tttagaaagt ggtttagatg agataataga aaaattcttg atctccgaca acgagtactt
     8101 ttattttttt tgctaatcac tttactcaat attagctcga aatcgtagaa acgtagacgg
     8161 gtgcgggata ccgagtggtg tagttaagaa tttttataaa ccacgtggcc caaaaatatg
     8221 aacccaaaac gtttatacat gagtatactt taagaaggct ataccccttc gtgttagatg
     8281 tagttttagc tacccaaccc gagtctatga gcttgacttc agatgtagaa ggcattaaat
     8341 cgttttgaat attaattaaa aaacgatgaa aattaaatat ttaaaagcaa tcatacgctg
     8401 aaaatttagt gctgtggcta atccttcaac atggaaatgc cataaaagtg actttgacaa
     8461 aaaaaaaagt atatacaggt agtaaactca tctacttcat tgactttgtt tacagcatgt
     8521 ggaaggagga atatttattg ctaaatcgta gtttaacatt caataagtaa tactattgaa
     8581 attcgacaag attggccgca tggatgaaaa agaggcattt tgctttggga gaattagtcc
     8641 aaattagaac tgaaaaaaaa aactttacga ggcaaaaatg tcggattgag atcgtaaaag
     8701 ttcgctcgtc gtcttttgct ttgtgattgt tttcatggat acatcttgct ggatatttaa
     8761 attttagtac tatgtataag atattctata aatgttttat cacccaaacc tgttagcgcc
     8821 ttcttaattc tattcaatct ggcttttgct ctgagactac ttcttggact ttcactactt
     8881 gttagttata cggaatttgt gtaattagaa gtgaaataat cctttctatt agtaatgcga
     8941 gctcgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa
     9001 taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta
     9061 catttagccc atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac
     9121 ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct
     9181 cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga
     9241 tttgccactg aggttcttct ttcatatact tcctttttaa atcttgctag gatacagttc
     9301 tcacatcaca tccgaacata aacaaccatg ggtaccactc ttgacgacac ggcttaccgg
     9361 taccgcacca gtgtcccggg ggacgccgag gccatcgagg cactggatgg gtccttcacc
     9421 accgacaccg tcttccgcgt caccgccacc ggggacggct tcaccctgcg ggaggtgccg
     9481 gtggacccgc ccctgaccaa ggtgttcccc gacgacgaat cggacgacga atcggacgac
     9541 ggggaggacg gcgacccgga ctctcggacg ttcgtcgcgt acggggacga cggcgacctg
     9601 gcgggcttcg tggtcgtctc gtactccggc tggaaccgcc ggctgaccgt cgaggacatc
     9661 gaggtcgccc cggagcaccg ggggcacggg gtcgggcgcg cgttgatggg gctcgcgacg
     9721 gagttcgccc gcgagcgggg tgccgggcac ctctggctgg aggtcaccaa cgtcaacgca
     9781 ccggcgatcc acgcgtaccg gcggatgggg ttcaccctct gcggcctgga caccgccctg
     9841 tacgacggca ccgcctcgga cggcgagcag gcgctctaca tgagcatgcc ctgcccctaa
     9901 tcagtactga caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt
     9961 atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg
    10021 acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta
    10081 tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta acgccgccat ccacctgcag
    10141 gtaatcacgt gccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
    10201 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    10261 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10321 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10381 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10441 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10501 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10561 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10621 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10681 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10741 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10801 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10861 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10921 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10981 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    11041 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    11101 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    11161 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    11221 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    11281 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    11341 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    11401 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    11461 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11521 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11581 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11641 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11701 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11761 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11821 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11881 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11941 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    12001 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    12061 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    12121 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    12181 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    12241 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    12301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    12361 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    12421 tcgtc
//
