LOCUS       sequence-263650-       10583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mTagBFP2 tagged beta-tubulin to monitor microtubules.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10583)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 10583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0770
FEATURES             Location/Qualifiers
     source          1..10583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2697..3152)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2834..2855)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3046..3065
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3278..3300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3292..3309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3293..3309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3319..3338
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3319..3337
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4014..4724
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     gene            6686..8261
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        6686..7029
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7030..8058
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMRLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVGASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      8064..8261
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8323..8343)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8323..8341)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8362..8378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8362..8378)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8375..8397)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8386..8402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8410..8416,8417..8434,8435..8440))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8455..8476
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8593..8610)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8764..9352)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8844..8863)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9523..10314,10315..10383))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10146..10165
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10384..10488)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10556..10574
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttaaa cgttgcttta tatatggata acgacatata
      601 catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct gctttaaagt
      661 tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc aaaactcaaa
      721 taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat tcgttttttc
      781 aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca tcgaatatga
      841 gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg atggtagagg
      901 cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct cctgccaaac
      961 aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat gcaattgttg
     1021 aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac actgatgcct
     1081 tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg cgatgcattc
     1141 aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt cctgaatttt
     1201 gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt tatccttttg
     1261 tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt catcaaccat
     1321 ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt gaaaagttcg
     1381 ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga aaagtttatg
     1441 cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta tatgcccctg
     1501 ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat gcggtgcgca
     1561 cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat ggtgagacca
     1621 ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc attgaagctt
     1681 gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct ttcagcgaaa
     1741 tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt ttaggtactg
     1801 atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc ggtgcaactt
     1861 tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac gttaccatca
     1921 tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac gtcaaagatc
     1981 ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta agcaagatga
     2041 aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact attgtttcag
     2101 ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca agagggttgc
     2161 gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt gctgcaatga
     2221 cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga gttgactctc
     2281 ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct atcaataata
     2341 gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc tcccttttgg
     2401 ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag gctgataaat
     2461 tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata actgttaaat
     2521 gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga ttgtaaaatg
     2581 tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt gctgcgacgc
     2641 agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     2701 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2761 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2821 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2881 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2941 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3001 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3061 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3121 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     3181 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3301 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccaaa
     3361 taaatcaaaa ctttttccgc gtaactctag tataagccgg aacaaggcta agaaacaaat
     3421 aaatgcaacg tttttatatt tcgatctatc aaatcctcaa ttacggaagg gcaattagta
     3481 gcgacacaag agatatacgc tgctgacaag tttttcgtcg taataaaaga gcttttgaag
     3541 agaaaaaaat tacggcggag cgttgcaatt cgttatatat aatgtgctat gcataaactc
     3601 tacttttgct aggaattctc cttgccctta tgtaatattt tatatacttt taaaaagatt
     3661 tacatgagga tatacctgtt ctataaagga gcacatgttg agttttaatg gaaatttacc
     3721 aatccaatct ttcacttaca aattgagaaa ataaaaggtg aaattgatga agatcctaat
     3781 ctttttatga ttagaaagct taaaacacct gactgagtaa gcttcttttt gcaagcaatg
     3841 cattatttta atgtctattt acatgtttag catacttcct tccgtatcat caataaatat
     3901 cgttagactt ctttattaag gaattgtttt gattaagtgt acaccgcact tcactacaaa
     3961 ccaaacacca aaaacctcgt agaaaccctg gaaagacgtt cgaattaatt aaaatggtgt
     4021 ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     4081 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     4141 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     4201 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     4261 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     4321 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     4381 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     4441 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     4501 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata
     4561 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     4621 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     4681 ccagatactg cgacctccct agcaaactgg ggcacaagct taatgtcgac atgagagaga
     4741 tcatttccat tcacgttggc caagcaggaa ctcaaatcgg aaatgcttgt tgggaactgt
     4801 attgtttaga acatggtatt caaccaaatg gttacatgaa tcctgagact gcatcccaaa
     4861 atagtgatgg cgggttttct acttttttct cagaaactgg tcaaggaaaa tacgttccta
     4921 gaagtattta tgtagatctt gaaccgaatg tgatcgacca agttcgtact ggcccatacc
     4981 gtgatctttt ccatccagag caattaatta cgggcaagga agatgcatca aacaattatg
     5041 ctcgtggtca ctacactgtt ggaaaagagc tggttgatga agtaactgac aagattcgtc
     5101 gtattgccga taattgctcc ggccttcagg gctttctcgt cttccattcg tttggaggtg
     5161 gtaccggttc aggctttggt gcccttttac ttgaacgctt ggccatggaa tacacgaaaa
     5221 aatcaaagct tcaattttct gtgtatccgg ctcctcaagt tagtacttct gtagttgaac
     5281 cttataactc cgttttgact acccatgcta cgttggacct tgctgactgt acatttatgg
     5341 ttgacaacga atcgtgctat gatatttgcc gtagaaactt agatattgag cgtccttcct
     5401 atgagaatct caatagattg atcgctcagg ttgtttcttc catcaccgcc tctcttcgct
     5461 tcgagggcag cttgaatgtt gatttggcag aattccaaac gaatttggta ccttatcctc
     5521 gtattcattt ccctctcgtc acttatgcac caattgttag tgccgccaag gccttccatg
     5581 aatctaacag cgttcaagaa atcaccaatc aatgttttga accctacaac caaatggtta
     5641 aatgtgatcc tcgtgccggt cgctacatgg caacgtgttt gctgtatcgt ggtgatgtta
     5701 tccctcgcga tgttcaagct gctgtaacga ccattaaggc caagcgtaca attcagttcg
     5761 ttgactggtg tcctactgga ttcaaaatcg gtatttgtga tcgtccaccc caacacattg
     5821 aaggtagtga aattgctaag gttgaccgtg ccgtctgtat gctctccaat actacctcta
     5881 ttgcggaggc ttggtcaagg ctcgatcaca aatttgatct catgtatagc aagcgtgctt
     5941 ttgttcattg gtatgttggt gaaggtatgg aagaaggcga gttttctgaa gctcgtgagg
     6001 atcttgctgc tcttgaacgc gattatgagg aagttggtca agattcgatg gaagtcgatt
     6061 acatggaaga agagtactaa agaatttcgt ttatatgctt taaagatttt catttaataa
     6121 ccttctttta tacgtattat attttttttt tactttttct caatctctct cacactctct
     6181 acatgacata tacaaaattg ctaatactac ttttctttta ccttacaaat gttgcaatcg
     6241 gtgaacgctg tcaaagttta ttcacaataa ctagaagtat aaattttctt ttgacattcc
     6301 catgcaacta tgcattccat tcctttacgt gtctttcttt ctggttgcat cgtcattaga
     6361 ttaaacttgt gctattatgg ttgcaaccac ttgtttactg cactcttttt tggttctaca
     6421 gctttccccg attagtttac attttcaata cccatatttt tttatcctat tagatatccg
     6481 taaactgttt tgaaactaat ttctcttccg agtcgtgtta agaatagaaa tagccgttta
     6541 acacttgcgt aagcatatac tttctgtttg taaagcttgg ggatatttaa ttggaatttt
     6601 ttgtatataa taattgtgag attgctaaac gaaataatgt gagctcgttt agcttgcctc
     6661 gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct
     6721 tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc
     6781 ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta
     6841 cggctcctcg ctgcggacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg
     6901 tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt
     6961 ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca
     7021 taaacaacca tgggtaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     7081 gaaaagttcg acagcgtctc cgacctgatg cggctctcgg agggcgaaga atctcgtgct
     7141 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     7201 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     7261 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     7321 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     7381 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     7441 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     7501 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcggtgcgtc cgtcgcgcag
     7561 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     7621 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     7681 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     7741 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     7801 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     7861 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     7921 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     7981 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     8041 ccgagggcaa aggaataatc agtactgaca ataaaaagat tcttgttttc aagaacttgt
     8101 catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt
     8161 atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa
     8221 tatcatgcgt caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac
     8281 gccgccatcc acctgcaggt aatcacgtgc cagcttttgt tccctttagt gagggttaat
     8341 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8401 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8461 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8521 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8581 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8641 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8701 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8761 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8821 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8881 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8941 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9001 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9061 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9121 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9181 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9241 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9301 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9361 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9421 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9481 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9541 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9601 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9661 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9721 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9781 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9841 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9901 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9961 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10021 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10081 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10141 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10201 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10261 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10321 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10381 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10441 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10501 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10561 gcgtatcacg aggccctttc gtc
//
