LOCUS       sequence-263651-       12416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mCherry tagged PCNA to monitor DNA replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 12416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0785
FEATURES             Location/Qualifiers
     source          1..12416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1640..2434
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      complement(2926..3381)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3063..3084)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3275..3294
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3507..3529
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3521..3538
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3522..3538
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3567
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3548..3566
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3591..3607
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3592..3608
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6119..6826
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6264..6282)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6532..6551
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     gene            8975..10094
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        8975..9318
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             9319..9891
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      9897..10094
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(10156..10176)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10156..10174)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10195..10211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10195..10211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10208..10230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10219..10235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10243..10249,10250..10267,10268..10273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10288..10309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10426..10443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10597..11185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10677..10696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11356..12147,12148..12216))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11979..11998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12217..12321)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12389..12407
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcgctt acataatgag tgagtgtcat tattgaggaa tgccatgtca
      721 gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt tttgcccaag
      781 gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc actcttttta
      841 gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg cgtattggat
      901 atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat gagcaaactg
      961 cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat cgcatggaag
     1021 atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa attttgattc
     1081 catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg ctatatgtat
     1141 gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc tttctctaaa
     1201 taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt atttgtttgt
     1261 atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc atggtgaaaa
     1321 agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag ccttaatgcc
     1381 cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt gaatatattt
     1441 tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt ttgtctttat
     1501 aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat ctctgcacat
     1561 gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg atcgaaatta
     1621 aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag ctagagctga
     1681 ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa agcaaagcaa
     1741 cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg tagataaaat
     1801 tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt tcgaccagga
     1861 tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct ttgaggatcg
     1921 caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg tgtacaaaat
     1981 tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta ttatacaagg
     2041 cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg aaatgtcttc
     2101 caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt ttgagaagca
     2161 taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc aaagcgacta
     2221 cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg gacagcaata
     2281 tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg ttggtcgtgg
     2341 agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag aagctggttg
     2401 gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa aatttttttg
     2461 gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg ttttatgtca
     2521 gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta gcgactaaaa
     2581 tattaactat tatagataaa caccttggga ataaaaagta atttgctata gtaatttatt
     2641 aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg aataagaaaa
     2701 gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa agatgtatgt
     2761 agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa acattggtgt
     2821 tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt gctagggcgg
     2881 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     2941 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3001 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3061 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3121 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3181 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3241 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3301 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3361 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3421 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3481 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3541 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga
     3601 cggtatcgat aagcttgata tcgaattcct gcagcccaac tgcggtcaag atatttcttg
     3661 aatcaggcgc cttagaccgc tcggccaaac aaccaattac ttgttgagaa atagagtata
     3721 attatcctat aaatataacg tttttgaaca cacatgaaca aggaagtaca ggacaattga
     3781 ttttgaagag aatgtggatt ttgatgtaat tgttgggatt ccatttttaa taaggcaata
     3841 atattaggta tgtagatata ctagaagttc tcctcgacaa gcttgcatgc agaactggat
     3901 tatttttcgt tcttctagaa agtacgcgaa ttgtacgacg aatttcatct tcacgtccga
     3961 taactggatc cagttgacca tttctagcga gctctgtaag atcaacagta aatttattta
     4021 gtgcatcaaa tccttcctcg gcattctttg aatcgattct tttattacca cggacgttat
     4081 ttacagcaaa ttcaaacgct ttcggggtga caccggcttc tgccaataaa ctctttagcg
     4141 tgtcgtcttt agtgaacaca gcgataaaat gatcttgagc aatgtaagaa tctttttgtg
     4201 ttttttgaag ttcatgagca tttcgcagca gtttcgcact ttcaggtgac agagtcactt
     4261 gttctggagg tggatcctga gcaggcaagc gaactaaccg actagtaaca cttcgctcaa
     4321 acttttgacc atcaccacca gctttgtcga cgatggtgcg cagtaaggta gtaccgttac
     4381 tgtcactgtc ggacaaaaga gcagcagcaa tgtgaatagg ggttaattgt gaatgaccat
     4441 aagattgtgc aattgagtac gcatcagaca atgtctttgc ggctttgtca gtaaaaggat
     4501 aatcagccat atcgagttat attattatag gaatttttat aatttataga taaatccaaa
     4561 aaaaaggaat tgtatatttg tcgctcgtta taaaacaaaa aagagagagt cgttgaacgg
     4621 taaatatata cggttttcag ttcgatttaa taagaactta atatcgttat tataataaat
     4681 tcttcggtta tagattgatg gttgaggatg ccgcaggtag agaagctgtg catatatata
     4741 cacgatgatg ttactatggg aaggaaagga acataccaga agatgccagt tacattgtta
     4801 ctcgaaccta ctcctacact ctagaaaaag aaagggataa agtagggtaa atcccaaaga
     4861 catgactaga agatttaaaa aaataagaga aaaatagctt aaaaaaaaac cggtgtaaat
     4921 taaaaatgca gttaccttaa aagtctttga attgtcttta aacatggaat actcgaaacc
     4981 cggaaatgat ctgcgtatta cgtagtgtat ggaacaacgt aaagacggat actaacctca
     5041 acttattctc aattgcatct tgctaaatac cgttagaatt catcaatgac tgaataaata
     5101 attttttgat acatagttct tctattacag agattctagt catatttcca agctatttct
     5161 aagatagagc tcttacatta cttattctat acggtagcga ttattcgtcg taatgacatt
     5221 taacgaactt tagttacttg accatagtgg tttataaact aaacaaacca acagtgaatt
     5281 cttgttaacg taactgtaat ctacgaatat tgagaagaaa aaaaaacgaa tgaatttgtt
     5341 gtgaatacat attttttcct tagcgaacaa gtatacttca ttcttatttt catacctctt
     5401 tagtcttatt taaaccagca ataaaattcg ttttaagttt tccgtgttaa taccaattaa
     5461 aataacgttt ttttcaatca ttttatatgt cattgcaacg tgaaaataag ttaataaatt
     5521 tcggtagcag ctaatatgga aaatgtacaa tttacttgat acattagtgt agcctaaatt
     5581 ttatttgatc ctggaaagta tattaaggta gaacgcgaaa gctctcctag tttacgccgt
     5641 aaattgtaat gactcgctga ttacatttgg cttattataa tccgtatagc tagaaaattt
     5701 cgataactga atattgcttc tttaaaaaga cataaataga accgtgaatt agtacagcaa
     5761 acgaaagata aaaatttcat agtaacatgc aaatactatt ttttcagtta gttttccata
     5821 attttgtata atatttcata tcaaacgcat ctaaaaagta cggacgaaat tacttgctat
     5881 tgtaaaaata aaaaaaataa caaaacaaat tataataacg tgaactgcta ttttggtctc
     5941 atatatgaaa ggttcttgag attttttacg ttgtcagcta gcaattttac gcacactatt
     6001 ctgtgctacg cgctctttga ctacttccca aaagccaact acaaaaggtt tttgcagtaa
     6061 cctttgttag ctattcttta tatctcttat ctagcttgta taaaatataa ttacgaccat
     6121 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     6181 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     6241 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     6301 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     6361 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     6421 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     6481 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     6541 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     6601 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     6661 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     6721 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     6781 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtccg gactcagatc
     6841 gggtgagggt caaggtcaag gtcaaggtcc tggtcgtggt tacgcttacc gtagtagatc
     6901 tatgcttgaa gctagatttc agcaggctgc tttgttgaaa aagcttctcg atgccatcaa
     6961 ggagttagtt acagatgcta attttgattg caatgacaac ggtatctctc tgcaggctat
     7021 ggatagttct catgtagccc ttgtttctat gcttattaaa tcagatggat ttgaaccata
     7081 tcggtgtgat agaaatattg ctttaggtat aaatctgaac gccttatcaa aggtcttacg
     7141 gtgtgcccaa aacgaggatt tggttacttt aaaggctgaa gatacacctg aggtattgaa
     7201 tttggttttc gagagcgaga aaaatgacag gatctctgat tatgatgtta aattaatgga
     7261 cattgatcaa gaacacttgg gtataccaga tatcgaatac gatgctacta ttactatgcc
     7321 tgctgccgaa tttcaacgca ttactcgaga tttattaact ttgagtgatt cagtaacaat
     7381 caatgctagt aaagagggtg ttcgttttag ctgcaaggga gatattggta atggtagtac
     7441 cacgctgaaa caacataccg atctttctga ccaagatcaa agtatcgaaa tttctcttac
     7501 ccaagctgta acacttacgt tttctttgaa atatttagcc cagttcacta aggctactcc
     7561 tttagctacc cgagtcacac tttcaatgag caatgatgtt ccacttcttg tggagtataa
     7621 aatggaatcc ggatttttac gattttatct tgccccaaaa attggtgagg aggatgagga
     7681 gtagattacg gaatcaatat cttttagtaa tctctttgca attgacgagc tagcatcatt
     7741 ataaacatac ttttgtttac tttttaattc aacctttttt gtactgctcc ttccctaaca
     7801 ttcatatcca aacaggcaaa ataccagctt ttacttaaaa aaaatcaaat tctttagaac
     7861 caacaaactg aagggtagaa aaacttgtgt tcttagtgat gatttttcac agataacttc
     7921 gtaatcggat ccccgggtaa aaggaatgtc tcccttgcca gtactgctag ggtttttctt
     7981 tcaaactatg gaagcccatt caagctgcat attacgattt tgtttttcgc ttttagaaag
     8041 tggtttagat gagataatag aaaaattctt gatctccgac aacgagtact tttatttttt
     8101 ttgctaatca ctttactcaa tattagctcg aaatcgtaga aacgtagacg ggtgcgggat
     8161 accgagtggt gtagttaaga atttttataa accacgtggc ccaaaaatat gaacccaaaa
     8221 cgtttataca tgagtatact ttaagaaggc tatacccctt cgtgttagat gtagttttag
     8281 ctacccaacc cgagtctatg agcttgactt cagatgtaga aggcattaaa tcgttttgaa
     8341 tattaattaa aaaacgatga aaattaaata tttaaaagca atcatacgct gaaaatttag
     8401 tgctgtggct aatccttcaa catggaaatg ccataaaagt gactttgaca aaaaaaaaag
     8461 tatatacagg tagtaaactc atctacttca ttgactttgt ttacagcatg tggaaggagg
     8521 aatatttatt gctaaatcgt agtttaacat tcaataagta atactattga aattcgacaa
     8581 gattggccgc atggatgaaa aagaggcatt ttgctttggg agaattagtc caaattagaa
     8641 ctgaaaaaaa aaactttacg aggcaaaaat gtcggattga gatcgtaaaa gttcgctcgt
     8701 cgtcttttgc tttgtgattg ttttcatgga tacatcttgc tggatattta aattttagta
     8761 ctatgtataa gatattctat aaatgtttta tcacccaaac ctgttagcgc cttcttaatt
     8821 ctattcaatc tggcttttgc tctgagacta cttcttggac tttcactact tgttagttat
     8881 acggaatttg tgtaattaga agtgaaataa tcctttctat tagtaatgcg agctcgttta
     8941 gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct
     9001 tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc
     9061 catacatccc catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag
     9121 caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc
     9181 gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact
     9241 gaggttcttc tttcatatac ttccttttta aatcttgcta ggatacagtt ctcacatcac
     9301 atccgaacat aaacaaccat gggtaccact cttgacgaca cggcttaccg gtaccgcacc
     9361 agtgtcccgg gggacgccga ggccatcgag gcactggatg ggtccttcac caccgacacc
     9421 gtcttccgcg tcaccgccac cggggacggc ttcaccctgc gggaggtgcc ggtggacccg
     9481 cccctgacca aggtgttccc cgacgacgaa tcggacgacg aatcggacga cggggaggac
     9541 ggcgacccgg actctcggac gttcgtcgcg tacggggacg acggcgacct ggcgggcttc
     9601 gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg tcgaggacat cgaggtcgcc
     9661 ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg ggctcgcgac ggagttcgcc
     9721 cgcgagcggg gtgccgggca cctctggctg gaggtcacca acgtcaacgc accggcgatc
     9781 cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg acaccgccct gtacgacggc
     9841 accgcctcgg acggcgagca ggcgctctac atgagcatgc cctgccccta atcagtactg
     9901 acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag
     9961 ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct
    10021 gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc
    10081 tggtcgctat actgctgtcg attcgatact aacgccgcca tccacctgca ggtaatcacg
    10141 tgccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
    10201 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    10261 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    10321 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    10381 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    10441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    10501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    10561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    10621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    10681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    11041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    11101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    11161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    11221 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    11281 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    11341 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11401 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11461 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    11521 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11581 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11641 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11701 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    11761 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    11821 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11881 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11941 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12001 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    12061 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    12121 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    12181 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12241 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12301 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    12361 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
