LOCUS       sequence-263657-        4068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC38A2
FEATURES             Location/Qualifiers
     source          1..4068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2193..2292)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2309..2328)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2310..2328)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2333..2349)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2333..2349)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2451..2470)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2462..3271
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2529..2548)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3418..4006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3907..3926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgaaga aggccgagat gggccggttc tctatcagcc ctgacgagga
      721 tagctcctct tacagctcca actccgactt taattactct tatcccacca agcaggccgc
      781 cctgaagtcc cactatgccg acgtggatcc tgagaaccag aatttcctgc tggagagcaa
      841 cctgggcaag aagaagtacg agacagagtt tcacccaggc accacaagct tcggcatgtc
      901 cgtgtttaac ctgtctaatg caatcgtggg aagcggaatc ctgggactgt cctatgccat
      961 ggccaacacc ggcatcgccc tgttcatcat cctgctgaca ttcgtgagca tcttttccct
     1021 gtactctgtg cacctgctgc tgaagaccgc caatgaggga ggaagcctgc tgtacgagca
     1081 gctgggctat aaggccttcg gcctggtggg caagctggca gcatccggct ctatcacaat
     1141 gcagaacatc ggcgccatgt ctagctacct gtttatcgtg aagtatgagc tgccactggt
     1201 catccaggcc ctgaccaata tcgaggataa gacaggcctg tggtacctga acggcaatta
     1261 tctggtgctg ctggtgtctc tggtggtcat cctgcccctg agcctgttca gaaatctggg
     1321 ctacctgggc tataccagcg gcctgtccct gctgtgcatg gtgttctttc tgatcgtggt
     1381 catctgcaag aagtttcagg tgccatgtcc tgtggaggcc gccctgatca tcaacgagac
     1441 aatcaatacc acactgaccc agcctacagc cctggtgcca gccctgtccc acaacgtgac
     1501 cgagaatgac tcttgcagac ctcactactt catctttaac tctcagacag tgtatgccgt
     1561 gccaatcctg atcttcagct tcgtgtgcca cccagccgtg ctgcccatct acgaggagct
     1621 gaaggatagg tcccggagaa ggatgatgaa cgtgagcaag atcagcttct ttgccatgtt
     1681 cctgatgtat ctgctggccg ccctgttcgg ctacctgacc ttttatgagc acgtggagag
     1741 cgagctgctg cacacctact cctctatcct gggcacagac atcctgctgc tgatcgtgcg
     1801 cctggccgtg ctgatggcag tgaccctgac agtgcccgtg gtcatcttcc ctatccgcag
     1861 ctccgtgacc cacctgctgt gcgcctctaa ggactttagc tggtggcggc acagcctgat
     1921 caccgtgtcc atcctggcct tcacaaacct gctggtcatc tttgtgccca caatccggga
     1981 tatcttcggc tttatcggcg cctccgccgc ctctatgctg atcttcatcc tgccatccgc
     2041 cttttacatc aagctggtga agaaggagcc catgaagtct gtgcagaaga tcggcgccct
     2101 gttctttctg ctgagcggcg tgctggtcat gaccggctcc atggccctga tcgtgctgga
     2161 ttgggtgcac aatgcccctg gcggcggcca cgacccagct ttcttgtaca aagttggcat
     2221 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     2281 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     2341 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     2401 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     2461 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     2521 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     2581 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     2641 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     2701 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     2761 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     2821 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     2881 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     2941 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     3001 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     3061 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     3121 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     3181 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     3241 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     3301 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     3361 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
