LOCUS       sequence-263662-       11066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of 3GFP tagged RasAct probe to monitor active Ras1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11066)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 11066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0758
FEATURES             Location/Qualifiers
     source          1..11066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2526..2981)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2663..2684)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2875..2894
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3107..3129
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3121..3138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3122..3138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3148..3167
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3148..3166
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4933..5646
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             5653..6366
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             6373..7089
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      7131..7318
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7186..7210)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            7393..8749
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        7393..7736
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7737..8546
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7804..7823)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      8552..8749
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8806..8826)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8806..8824)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8845..8861)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8845..8861)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8858..8880)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8869..8885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8893..8899,8900..8917,8918..8923))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8938..8959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9076..9093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9247..9835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9327..9346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10006..10797,10798..10866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10629..10648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10867..10971)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11039..11057
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg gggccttacg aagttaaatt ataggcacag acatctttgg cattggaaag
      241 atttagtaac aatcataatg tagcacttct taaaaaatct aattttagaa caaaaaggct
      301 taaaaaagaa tggttccgct cttttaaaaa atagtaaaat gtacaatagc aaggttttcg
      361 taggtcgacg gacataacaa ggggaaagag aaataaagca tgtaaacgtc aataaaacat
      421 ctttgattct ggatcaatca tatgccaaat gaagaaaaat agcatttata acaacatgaa
      481 accatatttg agacctttta acgatgaaaa tttattaaat aaaagataaa aaagaaagaa
      541 accgttcaag ttcaatgaaa atttcgtcgt gaagtagaaa ccaagataat ggaaaggctt
      601 cgcaaagcca caaattttcc aatagtcatt ataactgaag attaaggaca gtatactagt
      661 aaaaatcaag tgaattgtgc ctaggatagg tagctaacgt gactgccact gaacctgtgt
      721 agcgtattag atccatcaag atcccttctg ctcatatcat tgctgatctg taaattgacc
      781 gcatcaaatt tgatctcatt tatatcgaga ttgatctata ttattttgcc aatttgcagt
      841 atataaactt atttgattaa gtttttggtc tccttcgcca cgtcttcatt ttgttagttt
      901 aaattttttt gactaactta gaaagggaag tttttaaaaa cgttgaaata ctttttcgat
      961 ttaaaaatat atatttaatt aattttctgt tccaaaatgg ttgctcctca tctttggtta
     1021 cgtgctgaaa ccaaaccttt agaagagcgc agtgctttga ctcctagaac tgccaagata
     1081 ctcgccgatg ctggatttca aattaccatt gagcgctctt cccaacgtgc tttcaaggac
     1141 aaagaatttg agcgccttgg tttccccatg gttcctgagg gctcatggcg acatgcacct
     1201 aaggacgcgt acattattgg tctcaaagag cttccggaaa atgataacag tcctttgaag
     1261 catactcata ttcaatttgc tcattgctac aaaaaccagg aaggttggcg tgaagtcttg
     1321 tcccgattcc ccgctggcaa tggtcttcta tatgatttgg aatttttgca agatgacaat
     1381 ggcagacgtg ttgccgcttt tggttaccac gctggttttg ctggatcagc tatctcatgt
     1441 ttagtttggg cacatcaatt attgcatcct aacaaacaat tccctgctat ccgtccgttc
     1501 cccaacgaaa aatctttggt tcgccatgtt gctcgtcaag tacgtttagc tttgaaaaag
     1561 aataacaacc agtatcctcg tattctggtt attggggctc ttggtcgttg tggtactgga
     1621 gcgtgcgatt tagcatcaaa gattggtatt cccttcgata acattcttcg ctgggacatt
     1681 aatgaaacta agaagggtgg tccctttacc gaaattacgg aatcagacat atttgttaac
     1741 tgcatttatc tgagcatgcc tattcccaaa ttctgtaccg tcgagtcgtt aaatgtacct
     1801 aaccgtaaat tgcgtgttgt ttgtgatgtt agctgtgaca ctaccaatcc taacaatcct
     1861 attccaattt ataatgtaaa cactacattt gatcatccta cagttgaggt taaaggtgtc
     1921 acaactcctc ctcctttaga ggtcatttct attgatcatc ttcctacttt attgccaagg
     1981 gaatcttccg aagcatttag tgaagctcta attcctagtc ttttggcttt gaaggacgtc
     2041 gacaatgctc ccgtttgggt cagagctaaa aaactttatg aaaccatggt tcaaaagttt
     2101 taaaataaat gttatttaat ttcaaagttc cgggtccttt atattgtttc accaaattat
     2161 tgaatattat tttctcaaaa attaacttta gaatacaatt tagacggcat tttcgatgct
     2221 ctggacattt tttccacgta cttgttaaat tgtagcggtg ttgcataata ctaccttttg
     2281 atttgttatt cacgttacaa tatatatttg actaatttaa atatgataat atacacattt
     2341 ctctgcaaac ctcctttata cttttcgccg gtcattgatt aataattaag aaacaggatt
     2401 tcatcctgca tttgccactt acctatatgg taaatataaa tcttgaaact ttccccaata
     2461 tttattgctt gctagggcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2521 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2581 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2641 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2701 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     2761 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     2821 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     2881 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     2941 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg
     3001 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     3061 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     3121 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc
     3181 gggccccccc tcgaggtcga ctcaaattca ctgatttaag actttataac aaaaagatct
     3241 tttttataaa tcctaactga ttagtgaaat taatataaga taatatgctt gctggtgaat
     3301 attgaatcaa aacttgctac tgaagtgata gtagcattca accctacgct tagaacttga
     3361 tcttcaccaa caacccagta acctacttta tacttcttta tgcgccagcg agtataccac
     3421 cgaagtctta taagttttgt ttaatttaga actatacatt aaactatcaa gaaaattatc
     3481 caagtaaaca gtgctgacgc gaccttcgtg ttctagttta tcatttatag agatttagtc
     3541 aattgagtag attctttgat attcttttct ttttgaaaag gatctatttc aaaagtttcc
     3601 ttttcggttg ttaatctgta caactgattc ctcataaaag tggcagcggc acaagttact
     3661 gtgccctttg cgcctcttac taggagaaaa atccttttca acttaagtgc tctattcttg
     3721 tctttttgtt ttttttttgc atactgaaac ttattccttc gttctgcaac acatttgcca
     3781 tagtcctatt tctgtttttc tttacccttt tcgttaataa atttatttac tatgggaggt
     3841 cccgggcgag agtttccacg tccatgtata ctgaggttta ttgcctgtaa cgggcaaact
     3901 cgagcagttc aatcacgagg agattaccag aaaacactcg ctattgcttt gaaaaagttt
     3961 agccttgaag atgcttcaaa attcattgta tgcgtttcac agagtagtcg aattaagctg
     4021 attactgaag aagaatttaa acaaatttgt tttaattcat cttcaccgga acgcgacagg
     4081 ttaattattg tgccaaaaga aaagccttgt ccatcgtttg aagacctccg ccgttcttgg
     4141 gagattgaat tggctcaacc ggcagcatta tcatcacagt cctccctttc tcctggatcc
     4201 cgagagtttc cacgtccatg tatactgagg tttattgcct gtaacgggca aactcgagca
     4261 gttcaatcac gaggagatta ccagaaaaca ctcgctattg ctttgaaaaa gtttagcctt
     4321 gaagatgctt caaaattcat tgtatgcgtt tcacagagta gtcgaattaa gctgattact
     4381 gaagaagaat ttaaacaaat ttgttttaat tcatcttcac cggaacgcga caggttaatt
     4441 attgtgccaa aagaaaagcc ttgtccatcg tttgaagacc tccgccgttc ttgggagatt
     4501 gaattggctc aaccggcagc attatcatca cagtcctccc tttctccttc tagacgagag
     4561 tttccacgtc catgtatact gaggtttatt gcctgtaacg ggcaaactcg agcagttcaa
     4621 tcacgaggag attaccagaa aacactcgct attgctttga aaaagtttag ccttgaagat
     4681 gcttcaaaat tcattgtatg cgtttcacag agtagtcgaa ttaagctgat tactgaagaa
     4741 gaatttaaac aaatttgttt taattcatct tcaccggaac gcgacaggtt aattattgtg
     4801 ccaaaagaaa agccttgtcc atcgtttgaa gacctccgcc gttcttggga gattgaattg
     4861 gctcaaccgg cagcattatc atcacagtcc tccctttctc ctttaattaa cggaacaaaa
     4921 gctggaggat ccatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
     4981 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
     5041 gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca
     5101 tggccaacct tagtcactac tttcggttat ggtgttcaat gttttgcgag atacccagat
     5161 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
     5221 actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt
     5281 gataccttag ttaatagaat cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt
     5341 ttaggtcaca aattggaata caactataac tctcacaatg tttacatcat ggctgacaaa
     5401 caaaagaatg gtatcaaagt taacttcaaa attagacaca acattgaaga tggttctgtt
     5461 caattagctg accattatca acaaaatact ccaattggtg atggtccagt cttgttacca
     5521 gacaaccatt acttatccac tcaatctgcc ttatccaaag atccaaacga aaagagagac
     5581 cacatggtct tgttagaatt tgttactgct gctggtatta cccatggtat ggatgaattg
     5641 tacaaaagat ccatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
     5701 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
     5761 gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca
     5821 tggccaacct tagtcactac tttcggttat ggtgttcaat gttttgcgag atacccagat
     5881 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
     5941 actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt
     6001 gataccttag ttaatagaat cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt
     6061 ttaggtcaca aattggaata caactataac tctcacaatg tttacatcat ggctgacaaa
     6121 caaaagaatg gtatcaaagt taacttcaaa attagacaca acattgaaga tggttctgtt
     6181 caattagctg accattatca acaaaatact ccaattggtg atggtccagt cttgttacca
     6241 gacaaccatt acttatccac tcaatctgcc ttatccaaag atccaaacga aaagagagac
     6301 cacatggtct tgttagaatt tgttactgct gctggtatta cccatggtat ggatgaattg
     6361 tacaaaagat ccatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
     6421 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
     6481 gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca
     6541 tggccaacct tagtcactac tttcggttat ggtgttcaat gttttgcgag atacccagat
     6601 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
     6661 actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt
     6721 gataccttag ttaatagaat cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt
     6781 ttaggtcaca aattggaata caactataac tctcacaatg tttacatcat ggctgacaaa
     6841 caaaagaatg gtatcaaagt taacttcaaa attagacaca acattgaaga tggttctgtt
     6901 caattagctg accattatca acaaaatact ccaattggtg atggtccagt cttgttacca
     6961 gacaaccatt acttatccac tcaatctgcc ttatccaaag atccaaacga aaagagagac
     7021 cacatggtct tgttagaatt tgttactgct gctggtatta cccatggtat ggatgaattg
     7081 tacaaataaa gatctgtatt ctttaatatc ggcgcgccac ttctaaataa gcgaatttct
     7141 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     7201 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     7261 tcaggttgct ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca
     7321 gatccgctag ggataacagg gtaatataga tctgtttagc ttgcctcgtc cccgccgggt
     7381 cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc
     7441 tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca
     7501 tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg
     7561 cagacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg
     7621 cccctgtaga gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt
     7681 ccttttaaaa tcttgctagg atacagttct cacatcacat ccgaacataa acaaccatgg
     7741 gtaaggaaaa gactcacgtt tcgaggccgc gattaaattc caacatggat gctgatttat
     7801 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
     7861 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     7921 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     7981 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggca
     8041 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     8101 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     8161 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     8221 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     8281 agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     8341 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     8401 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     8461 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     8521 atttgatgct cgatgagttt ttctaatcag tactgacaat aaaaagattc ttgttttcaa
     8581 gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag
     8641 cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca
     8701 gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg
     8761 atactaacgc cgccatccag tttaaacgag ctccagcttt tgttcccttt agtgagggtt
     8821 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8881 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8941 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9001 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9061 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9121 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9181 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9241 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9301 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9361 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9421 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9481 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9541 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9601 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9661 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9721 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9781 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9841 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9901 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9961 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10021 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10081 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10141 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10201 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10261 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10321 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10381 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10441 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10501 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10561 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10621 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10681 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10741 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10801 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10861 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10921 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10981 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    11041 taggcgtatc acgaggccct ttcgtc
//
