LOCUS       sequence-263692-       10580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mCherry tagged beta-tubulin to monitor microtubules.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10580)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 10580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0710
FEATURES             Location/Qualifiers
     source          1..10580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2697..3152)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2834..2855)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3046..3065
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3278..3300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3292..3309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3293..3309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3319..3338
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3319..3337
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4014..4721
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4159..4177)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4427..4446
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     gene            6683..8258
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        6683..7026
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7027..8055
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMRLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVGASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      8061..8258
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8320..8340)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8320..8338)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8359..8375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8359..8375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8372..8394)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8383..8399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8407..8413,8414..8431,8432..8437))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8452..8473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8590..8607)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8761..9349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8841..8860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9520..10311,10312..10380))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10143..10162
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10381..10485)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10553..10571
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttaaa cgttgcttta tatatggata acgacatata
      601 catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct gctttaaagt
      661 tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc aaaactcaaa
      721 taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat tcgttttttc
      781 aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca tcgaatatga
      841 gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg atggtagagg
      901 cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct cctgccaaac
      961 aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat gcaattgttg
     1021 aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac actgatgcct
     1081 tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg cgatgcattc
     1141 aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt cctgaatttt
     1201 gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt tatccttttg
     1261 tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt catcaaccat
     1321 ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt gaaaagttcg
     1381 ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga aaagtttatg
     1441 cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta tatgcccctg
     1501 ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat gcggtgcgca
     1561 cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat ggtgagacca
     1621 ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc attgaagctt
     1681 gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct ttcagcgaaa
     1741 tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt ttaggtactg
     1801 atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc ggtgcaactt
     1861 tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac gttaccatca
     1921 tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac gtcaaagatc
     1981 ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta agcaagatga
     2041 aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact attgtttcag
     2101 ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca agagggttgc
     2161 gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt gctgcaatga
     2221 cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga gttgactctc
     2281 ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct atcaataata
     2341 gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc tcccttttgg
     2401 ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag gctgataaat
     2461 tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata actgttaaat
     2521 gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga ttgtaaaatg
     2581 tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt gctgcgacgc
     2641 agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     2701 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2761 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2821 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2881 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2941 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3001 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3061 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3121 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     3181 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3301 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccaaa
     3361 taaatcaaaa ctttttccgc gtaactctag tataagccgg aacaaggcta agaaacaaat
     3421 aaatgcaacg tttttatatt tcgatctatc aaatcctcaa ttacggaagg gcaattagta
     3481 gcgacacaag agatatacgc tgctgacaag tttttcgtcg taataaaaga gcttttgaag
     3541 agaaaaaaat tacggcggag cgttgcaatt cgttatatat aatgtgctat gcataaactc
     3601 tacttttgct aggaattctc cttgccctta tgtaatattt tatatacttt taaaaagatt
     3661 tacatgagga tatacctgtt ctataaagga gcacatgttg agttttaatg gaaatttacc
     3721 aatccaatct ttcacttaca aattgagaaa ataaaaggtg aaattgatga agatcctaat
     3781 ctttttatga ttagaaagct taaaacacct gactgagtaa gcttcttttt gcaagcaatg
     3841 cattatttta atgtctattt acatgtttag catacttcct tccgtatcat caataaatat
     3901 cgttagactt ctttattaag gaattgtttt gattaagtgt acaccgcact tcactacaaa
     3961 ccaaacacca aaaacctcgt agaaaccctg gaaagacgtt cgaattaatt aaaatggtga
     4021 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     4081 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     4141 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     4201 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     4261 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     4321 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     4381 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     4441 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     4501 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     4561 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     4621 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     4681 agggccgcca ctccaccggc ggcatggacg agctgtacaa ggtcgacatg agagagatca
     4741 tttccattca cgttggccaa gcaggaactc aaatcggaaa tgcttgttgg gaactgtatt
     4801 gtttagaaca tggtattcaa ccaaatggtt acatgaatcc tgagactgca tcccaaaata
     4861 gtgatggcgg gttttctact tttttctcag aaactggtca aggaaaatac gttcctagaa
     4921 gtatttatgt agatcttgaa ccgaatgtga tcgaccaagt tcgtactggc ccataccgtg
     4981 atcttttcca tccagagcaa ttaattacgg gcaaggaaga tgcatcaaac aattatgctc
     5041 gtggtcacta cactgttgga aaagagctgg ttgatgaagt aactgacaag attcgtcgta
     5101 ttgccgataa ttgctccggc cttcagggct ttctcgtctt ccattcgttt ggaggtggta
     5161 ccggttcagg ctttggtgcc cttttacttg aacgcttggc catggaatac acgaaaaaat
     5221 caaagcttca attttctgtg tatccggctc ctcaagttag tacttctgta gttgaacctt
     5281 ataactccgt tttgactacc catgctacgt tggaccttgc tgactgtaca tttatggttg
     5341 acaacgaatc gtgctatgat atttgccgta gaaacttaga tattgagcgt ccttcctatg
     5401 agaatctcaa tagattgatc gctcaggttg tttcttccat caccgcctct cttcgcttcg
     5461 agggcagctt gaatgttgat ttggcagaat tccaaacgaa tttggtacct tatcctcgta
     5521 ttcatttccc tctcgtcact tatgcaccaa ttgttagtgc cgccaaggcc ttccatgaat
     5581 ctaacagcgt tcaagaaatc accaatcaat gttttgaacc ctacaaccaa atggttaaat
     5641 gtgatcctcg tgccggtcgc tacatggcaa cgtgtttgct gtatcgtggt gatgttatcc
     5701 ctcgcgatgt tcaagctgct gtaacgacca ttaaggccaa gcgtacaatt cagttcgttg
     5761 actggtgtcc tactggattc aaaatcggta tttgtgatcg tccaccccaa cacattgaag
     5821 gtagtgaaat tgctaaggtt gaccgtgccg tctgtatgct ctccaatact acctctattg
     5881 cggaggcttg gtcaaggctc gatcacaaat ttgatctcat gtatagcaag cgtgcttttg
     5941 ttcattggta tgttggtgaa ggtatggaag aaggcgagtt ttctgaagct cgtgaggatc
     6001 ttgctgctct tgaacgcgat tatgaggaag ttggtcaaga ttcgatggaa gtcgattaca
     6061 tggaagaaga gtactaaaga atttcgttta tatgctttaa agattttcat ttaataacct
     6121 tcttttatac gtattatatt ttttttttac tttttctcaa tctctctcac actctctaca
     6181 tgacatatac aaaattgcta atactacttt tcttttacct tacaaatgtt gcaatcggtg
     6241 aacgctgtca aagtttattc acaataacta gaagtataaa ttttcttttg acattcccat
     6301 gcaactatgc attccattcc tttacgtgtc tttctttctg gttgcatcgt cattagatta
     6361 aacttgtgct attatggttg caaccacttg tttactgcac tcttttttgg ttctacagct
     6421 ttccccgatt agtttacatt ttcaataccc atattttttt atcctattag atatccgtaa
     6481 actgttttga aactaatttc tcttccgagt cgtgttaaga atagaaatag ccgtttaaca
     6541 cttgcgtaag catatacttt ctgtttgtaa agcttgggga tatttaattg gaattttttg
     6601 tatataataa ttgtgagatt gctaaacgaa ataatgtgag ctcgtttagc ttgcctcgtc
     6661 cccgccgggt cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga
     6721 cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca
     6781 tgtataatca tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg
     6841 ctcctcgctg cggacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc
     6901 ccacgccgcg cccctgtaga gaaatataaa aggttaggat ttgccactga ggttcttctt
     6961 tcatatactt ccttttaaaa tcttgctagg atacagttct cacatcacat ccgaacataa
     7021 acaaccatgg gtaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     7081 aagttcgaca gcgtctccga cctgatgcgg ctctcggagg gcgaagaatc tcgtgctttc
     7141 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     7201 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     7261 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     7321 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     7381 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     7441 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     7501 catgtgtatc actggcaaac tgtgatggac gacaccgtcg gtgcgtccgt cgcgcaggct
     7561 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     7621 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     7681 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     7741 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     7801 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     7861 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     7921 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     7981 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     8041 agggcaaagg aataatcagt actgacaata aaaagattct tgttttcaag aacttgtcat
     8101 ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata
     8161 ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat
     8221 catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga tactaacgcc
     8281 gccatccacc tgcaggtaat cacgtgccag cttttgttcc ctttagtgag ggttaattgc
     8341 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8401 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8461 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8521 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8581 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8641 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8701 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8761 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8821 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8881 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8941 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9001 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9061 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9121 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9181 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9241 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9301 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9361 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9421 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9481 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9541 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9601 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9661 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9721 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9781 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9841 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9901 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9961 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10021 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10081 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10141 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10201 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10261 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10321 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10381 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10441 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10501 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    10561 tatcacgagg ccctttcgtc
//
