LOCUS       sequence-263724-        8272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding nuclear localization of LAMA-F98 for GFP as a 
            SNAP-Tag-fusion and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Farrants H, Tarnawski M, Muller TG, Otsuka S, Hiblot J, Koch B, 
            Kueblbeck M, Krausslich HG, Ellenberg J, Johnsson K
  TITLE     Chemogenetic Control of Nanobodies.
  JOURNAL   Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 
            10.1038/s41592-020-0746-7.
  PUBMED    32066961
REFERENCE   2  (bases 1 to 8272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 
            10.1038/s41592-020-0746-7."
COMMENT     Sequence Label: pCDNA3.0_nucLAMA-F98 IRES EGFP
FEATURES             Location/Qualifiers
     source          1..8272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2323..2343
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2347..2367
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2371..2391
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2422..2446)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2475..3042
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2493..3044
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2637..2654)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2864..2883
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3047..3049,3050..3052,3053..3763)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3047..3049,3050..3052,3053..3763)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3092..3113)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3353..3372)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3700..3721
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(3824..3842)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3825..3842)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3862..3879)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3868..4092
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4138..4566
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4225..4244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4435..4456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4575..4595)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4580..4910
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4761..4896
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4823..4842
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4977..5771
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5031..5050)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5641..5660
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5945..6066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5982..6001)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6036..6055
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6115..6131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6115..6131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6128..6150)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6139..6155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6163..6169,6170..6187,6188..6193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6208..6229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6346..6363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6517..7105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6597..6616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7276..8067,8068..8136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7899..7918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8137..8241)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccag cggccgcacc
      901 atggataaag actgcgaaat gaaacgcact accctggaca gcccgcttgg taaactggaa
      961 ctgagcggtt gcgagcaggg cctgcacgaa atcattttcc tgggcaaagg tacgagcgct
     1021 gcggatgcgg tcgaagtacc ggctccggct gcggttctgg gtggcccgga gccgctgatg
     1081 caggctaccg catggctgaa cgcatatttt caccagccag aggcgattga agagttccct
     1141 gtaccggccc tgcatcaccc ggtatttcag caggaatcct ttacccgtca ggtactgtgg
     1201 aaactgctga aagtagtgaa attcggcgag gtgatctcct attcccatct ggcggcgctg
     1261 gctggcaacc cagcggcaac tgcggcggtg aaaaccgctc tgtctggtaa tccggtgcca
     1321 attctgatcc cgtgccaccg tgtggttcag ggcgacctgg acgtaggtgg ctacgaaggc
     1381 ggcctggctg tgaaagaatg gctgctggcg cacgaaggtc accgtctggg taaaccaggc
     1441 ctgggcggcc gcatggccca ggtgcagctg gttgagagcg gcggtgctct ggtgcagccg
     1501 ggcggtagcc tgcgtctgag ctgcgccgcg agcggcttcc cggtgaaccg ctacagcatg
     1561 cgttggtacc gtcaggcgcc gggcaaggag cgtgaatggg tggctggtat gagcagcgcc
     1621 ggtgaccgta gcagctacga agatagcgtg aaaggccgct tcaccatcag ccgtgacgat
     1681 gcccgcaaca ccgtgtatct gcagatgaac agcctgaagc cggaggacac cgcggtgtac
     1741 tattgcaacg tgaacgtggg ttttctgcct gccgatctcg cctggtttaa acgcaacacc
     1801 ttaaataaac ccgtgattat gggccgccat acctgggaat caatcggtcg tccgttgcca
     1861 ggacgcaaaa atattatcct cagcagtcaa ccgggtacgg acgatcgcgt aacgtgggtg
     1921 aagtcggtgg atgaagccat cgcggcgtgt ggtgacgtac cagaaatcat ggtgattggc
     1981 ggcggtcgcg tttatgaaca gttcttgcca aaagcgcaaa aactgtatct gacgcatatc
     2041 gacgcagaag tggaaggcga cacccatttc ccggattacg agccggatga ctgggaatcg
     2101 gtattcagcg aattccacga tgctgatgcg cagaactctc acagctattg ctttgagatt
     2161 ctggagcggc ggggcggcgg cggcggcatg atcagtctga ttgcggcgtt agcggtagat
     2221 cgcgttatcg gaatggaaaa cgccatgccg tggaacgaat actggggcca gggtacccag
     2281 gtgaccgtga gcagcaagtc cggactcaga tctcgagctg atccaaaaaa gaagagaaag
     2341 gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt aggatccacc
     2401 ggatctagat agtaaggatc cacccagctt tcttgtacaa agtggtgatc gcgtaaacta
     2461 cgggctgcag gaattccgcc cccccccccc taacgttact ggccgaagcc gcttggaata
     2521 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     2581 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     2641 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     2701 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     2761 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     2821 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     2881 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     2941 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
     3001 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aataccatgg tgagcaaggg
     3061 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     3121 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     3181 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     3241 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     3301 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     3361 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3421 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3481 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3541 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3601 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3661 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3721 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg
     3781 actagctagt agctaactcg agcatgcatc tagagggccc tattctatag tgtcacctaa
     3841 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     3901 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     3961 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4021 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4081 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4141 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4201 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4261 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg
     4321 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4381 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4441 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4501 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4561 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg
     4621 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4681 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4741 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4801 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4861 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4921 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     4981 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5041 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5101 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5161 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5221 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5281 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5341 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5401 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5461 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     5521 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5581 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5641 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5701 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5761 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     5821 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5881 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5941 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6001 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6061 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6121 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6181 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6241 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6301 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6361 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6421 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6481 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     6721 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6781 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6841 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6901 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     6961 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7021 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
