LOCUS       sequence-26374-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag NTRK3
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..82
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccttctct aggcgccggc cggatccaga attcgccacc atggggtctt caaaatctaa
       61 accaaaggac cccagccagc gccggcgcag gatccgaggt taccttgact acaaagacga
      121 tgacgacaag caattgacaa gtttgtacaa aaaagttggc atggatgtct ctctttgccc
      181 agccaagtgt agtttctggc ggattttctt gctgggaagc gtctggctgg actatgtggg
      241 ctccgtgctg gcttgccctg caaattgtgt ctgcagcaag actgagatca attgccggcg
      301 gccggacgat gggaacctct tccccctcct ggaagggcag gattcaggga acagcaatgg
      361 gaacgccagt atcaacatca cggacatctc aaggaatatc acttccatac acatagagaa
      421 ctggcgcagt cttcacacgc tcaacgccgt ggacatggag ctctacaccg gacttcaaaa
      481 gctgaccatc aagaactcag gacttcggag cattcagccc agagcctttg ccaagaaccc
      541 ccatttgcgt tatataaacc tgtcaagtaa ccggctcacc acactctcgt ggcagctctt
      601 ccagacgctg agtcttcggg aattgcagtt ggagcagaac tttttcaact gcagctgtga
      661 catccgctgg atgcagctct ggcaggagca gggggangcc aagctcaaca gccagaacct
      721 ctactgcatc aacgctgatg gctcccagct tcctctcttc cgcatgaaca tcagtcagtg
      781 tgaccttcct ganatcancg tgagccacgt caacctgacc g
//
