LOCUS       sequence-263740-       10265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of 3xmTagBFP2 targeted to the plasma membrane with an 
            amphipathic helix of RitC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 10265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0612
FEATURES             Location/Qualifiers
     source          1..10265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2697..3152)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2834..2855)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3046..3065
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3278..3300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3292..3309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3293..3309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3319..3338
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3319..3337
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4358..5068
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5075..5785
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5792..6502
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      6742..6929
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6797..6821)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        7004..7347
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      7746..7943
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8005..8025)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8005..8023)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8044..8060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8044..8060)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8057..8079)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8068..8084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8092..8098,8099..8116,8117..8122))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8137..8158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8275..8292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8446..9034)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8526..8545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9205..9996,9997..10065))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9828..9847
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10066..10170)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10238..10256
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttaaa cgttgcttta tatatggata acgacatata
      601 catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct gctttaaagt
      661 tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc aaaactcaaa
      721 taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat tcgttttttc
      781 aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca tcgaatatga
      841 gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg atggtagagg
      901 cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct cctgccaaac
      961 aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat gcaattgttg
     1021 aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac actgatgcct
     1081 tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg cgatgcattc
     1141 aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt cctgaatttt
     1201 gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt tatccttttg
     1261 tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt catcaaccat
     1321 ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt gaaaagttcg
     1381 ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga aaagtttatg
     1441 cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta tatgcccctg
     1501 ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat gcggtgcgca
     1561 cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat ggtgagacca
     1621 ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc attgaagctt
     1681 gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct ttcagcgaaa
     1741 tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt ttaggtactg
     1801 atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc ggtgcaactt
     1861 tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac gttaccatca
     1921 tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac gtcaaagatc
     1981 ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta agcaagatga
     2041 aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact attgtttcag
     2101 ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca agagggttgc
     2161 gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt gctgcaatga
     2221 cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga gttgactctc
     2281 ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct atcaataata
     2341 gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc tcccttttgg
     2401 ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag gctgataaat
     2461 tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata actgttaaat
     2521 gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga ttgtaaaatg
     2581 tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt gctgcgacgc
     2641 agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     2701 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2761 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2821 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2881 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2941 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3001 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3061 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3121 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     3181 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3301 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac ctaaagtatg
     3361 gaaaatcaaa attcctgaat ttttgtaaac tcccatacaa atacgattac acatacatac
     3421 aaaaatgcaa ataacaaaat atacacgcac gcatgcacac taaataaaca aactgtgcgt
     3481 ccattccttt tatgcacaaa cgttgaagct caggacatcg caccaacaat cactcatctc
     3541 ttctctctct acccactttc cacccacacc cacagtctgc atctgtttga cgactgtagc
     3601 tcaagcatca gtagcagccg ctgtcatcct caactgctgc tgctgatgct gcttgaatgt
     3661 acgcatgata gcaacgacgc accgctctac cactactacc gaagcgccat catcacctcc
     3721 tcacctacca tcgcaatcgt cctctcgaaa ctcttcgacc gctttcaccc ggcttcctgc
     3781 aggtaacaat gcgctcggcg cttgattaaa agaggccaga ggcgataaat gactcagggt
     3841 gcatttggcc tcgctacgaa aggaccctgt cttgtgattt ttccgtagcc aatggacaac
     3901 cgccttgtca accttttgac tttggaatgt gctagcaatt cctccttccc cctgacttca
     3961 ggtgacaagg cttgcatcac gctgaggtag gcgtcggaaa aactttcctc acaaggctga
     4021 cgtcatggaa tgtgcaatca aagtcaggag cgggaacgag gcagattacc aacgaagcaa
     4081 cgaccatctc ggaacaactt gcaaagaaag tcaaggaaag ttggcaccac atcacgatcg
     4141 cgcaccttct ccaatcacct tgctccttag ttgttccgaa tcgagtgcga ggcaattgtg
     4201 gtaaagatag tcgggggaaa cccttacccc attcacacca cggctgatgg ctgctgtgtg
     4261 ggatggaaag gtataaatag aggcaggcgg tgcctagcat cgcttctgta tagatcattc
     4321 atccatagta ttgatttaca cttgattcaa acctagtatg gtgtctaagg gcgaagagct
     4381 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
     4441 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     4501 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
     4561 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
     4621 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     4681 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     4741 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
     4801 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
     4861 cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca agaaacccgc
     4921 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga
     4981 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
     5041 ccctagcaaa ctggggcaca agcttaatgc tagtatggtg tctaagggcg aagagctgat
     5101 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     5161 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     5221 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     5281 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     5341 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     5401 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     5461 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     5521 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     5581 gggcgggagc catctgatcg caaacgccaa gaccacatat agatccaaga aacccgctaa
     5641 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     5701 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     5761 tagcaaactg gggcacaagc ttaatgctag tatggtgtct aagggcgaag agctgattaa
     5821 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg
     5881 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt
     5941 cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag
     6001 caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga
     6061 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
     6121 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
     6181 cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac
     6241 gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg
     6301 cgggagccat ctgatcgcaa acgccaagac cacatataga tccaagaaac ccgctaagaa
     6361 cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa
     6421 caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag
     6481 caaactgggg cacaagctta atgctagcgt cgaccacaaa aaaaagtcaa agtgtccctt
     6541 ttttgagaca tctgctgcat accgctacta tattgatgat gttttccatg cccttgtacg
     6601 ggagatacgt aggaaagaaa aggaggcagt actggccatg gagaaaaaat ctaagcccaa
     6661 aaacagtgta tggaagaggc taaaatcacc attccggaag aagaaagatt cagtaacttg
     6721 aggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa
     6781 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
     6841 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag
     6901 gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag ggtaatatag
     6961 atctgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa
     7021 taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta
     7081 catttagccc atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac
     7141 ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct
     7201 cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga
     7261 tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc
     7321 tcacatcaca tccgaacata aacaaccatg cctttgtctc aagaagaatc caccctcatt
     7381 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     7441 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     7501 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     7561 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgt
     7621 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     7681 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     7741 tcagtactga caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt
     7801 atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg
     7861 acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta
     7921 tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta acgccgccat ccacctgcag
     7981 gtaatcacgt gccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     8041 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8101 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8161 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8221 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8281 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8341 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8401 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8461 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8521 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8581 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8641 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8701 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8761 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8821 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8881 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8941 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9001 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9061 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9121 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9181 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9241 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9301 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9361 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9421 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9481 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9541 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9601 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9661 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9721 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9781 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9841 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9901 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9961 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10021 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10081 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10141 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10201 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    10261 tcgtc
//
