LOCUS       sequence-263746-        9743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of cytoplasmic 3mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 9743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0624
FEATURES             Location/Qualifiers
     source          1..9743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1640..2434
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      complement(2926..3381)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3063..3084)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3275..3294
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3507..3529
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3521..3538
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3522..3538
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3567
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3548..3566
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4603..5313
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5320..6030
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             6037..6747
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(7483..7503)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7483..7501)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7522..7538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7522..7538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7535..7557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7546..7562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7570..7576,7577..7594,7595..7600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7615..7636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7753..7770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7924..8512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8004..8023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8683..9474,9475..9543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9306..9325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9544..9648)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9716..9734
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcgctt acataatgag tgagtgtcat tattgaggaa tgccatgtca
      721 gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt tttgcccaag
      781 gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc actcttttta
      841 gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg cgtattggat
      901 atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat gagcaaactg
      961 cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat cgcatggaag
     1021 atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa attttgattc
     1081 catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg ctatatgtat
     1141 gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc tttctctaaa
     1201 taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt atttgtttgt
     1261 atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc atggtgaaaa
     1321 agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag ccttaatgcc
     1381 cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt gaatatattt
     1441 tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt ttgtctttat
     1501 aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat ctctgcacat
     1561 gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg atcgaaatta
     1621 aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag ctagagctga
     1681 ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa agcaaagcaa
     1741 cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg tagataaaat
     1801 tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt tcgaccagga
     1861 tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct ttgaggatcg
     1921 caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg tgtacaaaat
     1981 tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta ttatacaagg
     2041 cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg aaatgtcttc
     2101 caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt ttgagaagca
     2161 taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc aaagcgacta
     2221 cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg gacagcaata
     2281 tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg ttggtcgtgg
     2341 agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag aagctggttg
     2401 gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa aatttttttg
     2461 gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg ttttatgtca
     2521 gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta gcgactaaaa
     2581 tattaactat tatagataaa caccttggga ataaaaagta atttgctata gtaatttatt
     2641 aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg aataagaaaa
     2701 gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa agatgtatgt
     2761 agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa acattggtgt
     2821 tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt gctagggcgg
     2881 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     2941 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3001 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3061 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3121 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3181 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3241 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3301 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3361 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3421 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3481 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3541 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggcccgcta gcatgctaaa
     3601 gtatggaaaa tcaaaattcc tgaatttttg taaactccca tacaaatacg attacacata
     3661 catacaaaaa tgcaaataac aaaatataca cgcacgcatg cacactaaat aaacaaactg
     3721 tgcgtccatt ccttttatgc acaaacgttg aagctcagga catcgcacca acaatcactc
     3781 atctcttctc tctctaccca ctttccaccc acacccacag tctgcatctg tttgacgact
     3841 gtagctcaag catcagtagc agccgctgtc atcctcaact gctgctgctg atgctgcttg
     3901 aatgtacgca tgatagcaac gacgcaccgc tctaccacta ctaccgaagc gccatcatca
     3961 cctcctcacc taccatcgca atcgtcctct cgaaactctt cgaccgcttt cacccggctt
     4021 cctgcaggta acaatgcgct cggcgcttga ttaaaagagg ccagaggcga taaatgactc
     4081 agggtgcatt tggcctcgct acgaaaggac cctgtcttgt gatttttccg tagccaatgg
     4141 acaaccgcct tgtcaacctt ttgactttgg aatgtgctag caattcctcc ttccccctga
     4201 cttcaggtga caaggcttgc atcacgctga ggtaggcgtc ggaaaaactt tcctcacaag
     4261 gctgacgtca tggaatgtgc aatcaaagtc aggagcggga acgaggcaga ttaccaacga
     4321 agcaacgacc atctcggaac aacttgcaaa gaaagtcaag gaaagttggc accacatcac
     4381 gatcgcgcac cttctccaat caccttgctc cttagttgtt ccgaatcgag tgcgaggcaa
     4441 ttgtggtaaa gatagtcggg ggaaaccctt accccattca caccacggct gatggctgct
     4501 gtgtgggatg gaaaggtata aatagaggca ggcggtgcct agcatcgctt ctgtatagat
     4561 cattcatcca tagtattgat ttacacttga ttcaaaccta gtatggtgtc taagggcgaa
     4621 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
     4681 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     4741 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
     4801 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
     4861 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
     4921 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
     4981 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     5041 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
     5101 aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa
     5161 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
     5221 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
     5281 gacctcccta gcaaactggg gcacaagctt aatgctagta tggtgtctaa gggcgaagag
     5341 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac
     5401 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     5461 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc
     5521 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc
     5581 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
     5641 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     5701 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc
     5761 accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag
     5821 ctcgtgggcg ggagccatct gatcgcaaac gccaagacca catatagatc caagaaaccc
     5881 gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag
     5941 gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac
     6001 ctccctagca aactggggca caagcttaat gctagtatgg tgtctaaggg cgaagagctg
     6061 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
     6121 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     6181 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
     6241 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
     6301 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
     6361 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     6421 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
     6481 gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
     6541 gtgggcggga gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct
     6601 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag
     6661 gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
     6721 cctagcaaac tggggcacaa gcttaatgct agagctttta tgattttgat aaatactttt
     6781 tttcgttttg gtttgtgata cgtatagttt gcctgtctcc ttttgtaaat gatcttcttt
     6841 tgtagtcttg tgtaatctct ttttcatttg gtcaacttaa gtgaattttt ttttgtcagt
     6901 ttccttgtag gatattatgt caaagtctta tttatactat atattattgc tttggtggtg
     6961 agtcgcattt gtggggacgg tgttacttta ctgatggatt taggtaggag gcatccactg
     7021 tcccccattt cgctagctta agtttaatat gaagatgcac ttaagcatga acgcccgttt
     7081 tgatcttttt cacttgtacc gcgaagagct tgttttactg tttttccgtc tgcatcccga
     7141 gaactagttg atctcccaat gcacacttta gactttattc aaaccgaagt cggttttgta
     7201 atttaaaaat ttttacccac tgtaaagtac aaacaattca tcgctatgtt agttgtgtac
     7261 gctactagcg catcacatga ttacatgtta atcaacaaaa acgaaaatgt ttattgtcaa
     7321 tctaaaaata catatgtccg ttaagacaaa agtatgaggc tggatggaat gagcaatggt
     7381 agcaaaaggt ttccgaaatg gtcatatcat tttttcaatt tttgctgatc tcaaatacct
     7441 caacttgttt tattcgttta aaccgagctc cagcttttgt tccctttagt gagggttaat
     7501 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7561 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7621 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7681 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7801 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8341 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8521 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8581 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8641 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8701 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8761 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8821 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8881 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8941 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9001 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9061 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9121 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9181 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9241 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9301 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9361 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9421 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9481 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9541 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9601 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9661 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     9721 gcgtatcacg aggccctttc gtc
//
