LOCUS       sequence-263749-       11621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A binary plasmid for plant transformation containing _-glucuronidase
            gene driven by CaMV 35S promoter and a bialaphos resistance gene 
            (bar) driven by CaMV 35S promoter and TEV enhancer..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Frame BR, Shou H, Chikwamba RK, Zhang Z, Xiang C, Fonger TM, Pegg 
            SE, Li B, Nettleton DS, Pei D, Wang K
  TITLE     Agrobacterium tumefaciens-mediated transformation of maize embryos 
            using a standard binary vector system.
  JOURNAL   Plant Physiol. 2002 May;129(1):13-22. doi: 10.1104/pp.000653.
  PUBMED    12011333
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1104/pp"; 
            journalName: "Plant Physiol"; date: "2002-05"; volume: "129"; issue:
            "1"; pages: "13-22"
COMMENT     Sequence Label: pTF102
FEATURES             Location/Qualifiers
     source          1..11621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    181..202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(217..222,223..240,241..247)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    255..271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     260..282
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     279..295
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     279..295
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    304..360
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        450..794
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          1217..1405
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     promoter        3213..3557
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3709..4257
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     complement(4827..4844)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4827..4843)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4836..4858)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5058..5082
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5603..6394
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6640..7228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7129..7148
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7382..7399
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7414..7554
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7480..7499)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7640..7662
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7898..8092
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8158..9231)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9660..10289)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    join(11620..11621,1..23)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa
       61 tctgatccaa gctcaagctc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      121 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      181 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      241 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
      301 tacgaattcg agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt
      361 gcatgcctgc aggtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
      421 gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga
      481 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag
      541 gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
      601 tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa
      661 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
      721 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
      781 catttggaga ggacctcgac tctagaggat ccccgggtag gtcagtccct tatgttacgt
      841 cctgtagaaa ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg
      901 gatcgcgaaa actgtggaat tggtcagcgt tggtgggaaa gcgcgttaca agaaagccgg
      961 gcaattgctg tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat
     1021 gcgggcaacg tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt
     1081 atcgtgctgc gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa
     1141 gtgatggagc atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt
     1201 gccgggaaaa gtgtacgtaa gtttctgctt ctacctttga tatatatata ataattatca
     1261 ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat
     1321 agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat
     1381 atgaccaaaa tttgttgatg tgcaggtatc accgtttgtg tgaacaacga actgaactgg
     1441 cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac
     1501 ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg
     1561 aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg
     1621 tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat
     1681 caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac
     1741 ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca
     1801 gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag
     1861 ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac
     1921 ttgcgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg
     1981 attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg
     2041 gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct
     2101 ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc
     2161 aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa
     2221 aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt
     2281 gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg
     2341 atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt
     2401 gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg
     2461 gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt
     2521 atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg
     2581 tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc
     2641 agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata
     2701 ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg
     2761 gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga
     2821 ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt cggctacagc ctcgggaatt
     2881 gctaccgagc tcggtacccg gcgcaaaaat caccagtctc tctctacaaa tctatctctc
     2941 tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta gggttcttat
     3001 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     3061 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacc tgcaggcatg
     3121 caagcttgca tgccggtcaa catggtggag cacgacacac ttgtctactc caaaaatatc
     3181 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc
     3241 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa
     3301 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
     3361 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     3421 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
     3481 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
     3541 tttcatttgg agaggacctc gagaattaat tctcaacaca acatatacaa aacaaacgaa
     3601 tctcaagcaa tcaagcattc tacttctatt gcagcaattt aaatcatttc ttttaaagca
     3661 aaagcaattt tctgaaaatt ttcaccattt acgaacgggg gatctaccat gagcccagaa
     3721 cgacgcccgg ccgacatccg ccgtgccacc gaggcggaca tgccggcggt ctgcaccatc
     3781 gtcaaccact acatcgagac aagcacggtc aacttccgta ccgagccgca ggaaccgcag
     3841 gagtggacgg acgacctcgt ccgtctgcgg gagcgctatc cctggctcgt cgccgaggtg
     3901 gacggcgagg tcgccggcat cgcctacgcg ggcccctgga aggcacgcaa cgcctacgac
     3961 tggacggccg agtcgaccgt gtacgtctcc ccccgccacc agcggacggg actgggctcc
     4021 acgctctaca cccacctgct gaagtccctg gaggcacagg gcttcaagag cgtggtcgct
     4081 gtcatcgggc tgcccaacga cccgagcgtg cgcatgcacg aggcgctcgg atatgccccc
     4141 cgcggcatgc tgcgggcggc cggcttcaag cacgggaact ggcatgacgt gggtttctgg
     4201 cagctggact tcagcctgcc ggtaccgccc cgtccggtcc tgcccgtcac cgagatctgc
     4261 tcaacaatct agctagagtt tgctcctatc tatatgtaat aaggtatgct gatatgcact
     4321 attcaaatag gagcattagc tatgtttgtt aatgtcactt tatgttatgt gggtaagtca
     4381 cctaagacac tccacgtacc tacttgttgt ctcttacgcg gctttaataa atcttctgcc
     4441 cttgttccat atttactaat tatccctttc ttcactaaaa gaaaattgtt atcattaagt
     4501 attagtcttt agaacatatg aggtctttaa ttgggtaggt tttacaaatt aactaatata
     4561 aaatgtcata aaatccacgt ggttaaacaa atgcagaaaa tcgacgtcgt ctattggacc
     4621 gacagttgct attaatataa tgggccacca tagtagactg acaaataaat tacctgacaa
     4681 catcgtttca caaaaaaaca aacacaaaaa gggagtgcat tttccagggc atttttgtaa
     4741 taaaaaacag ttaaaaggga gtgcaataga aatatagggg tgtggaaata gtgatttgag
     4801 cacgtcttga agcgaattag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
     4861 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4921 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4981 tgctagagca attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
     5041 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     5101 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     5161 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     5221 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     5281 gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg
     5341 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     5401 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     5461 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     5521 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     5581 ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact
     5641 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     5701 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     5761 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     5821 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     5881 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     5941 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     6001 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     6061 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     6121 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     6181 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     6241 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     6301 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     6361 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc
     6421 gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc
     6481 acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct
     6541 acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca
     6601 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6661 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6721 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6781 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6841 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6901 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6961 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7021 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7081 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7141 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7201 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7261 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7321 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7381 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     7441 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     7501 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     7561 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     7621 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     7681 aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc
     7741 ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc
     7801 cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc
     7861 tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt
     7921 aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat
     7981 atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg
     8041 gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag
     8101 accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta
     8161 ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc
     8221 agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt
     8281 gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt
     8341 cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa
     8401 acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt
     8461 cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg
     8521 ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac
     8581 cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt
     8641 taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca
     8701 gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc
     8761 ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta
     8821 atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag
     8881 ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac
     8941 gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc
     9001 tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg
     9061 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
     9121 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
     9181 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
     9241 tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct
     9301 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
     9361 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
     9421 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
     9481 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
     9541 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
     9601 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
     9661 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
     9721 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
     9781 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
     9841 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
     9901 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
     9961 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
    10021 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
    10081 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
    10141 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
    10201 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
    10261 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
    10321 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
    10381 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
    10441 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
    10501 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
    10561 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
    10621 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
    10681 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
    10741 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
    10801 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
    10861 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
    10921 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
    10981 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
    11041 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
    11101 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
    11161 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc
    11221 gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta
    11281 atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa
    11341 acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc
    11401 gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg
    11461 ttggatcaaa gtactttaaa gtactttaaa gtactttaaa gtactttgat cccgagggga
    11521 accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt
    11581 aaatatccga ttattctaat aaacgctctt ttctcttagg t
//
