LOCUS       sequence-263750-        9803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nucleo-cytoplasmic sfGFP in M-cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 9803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0523
FEATURES             Location/Qualifiers
     source          1..9803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2697..3152)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2834..2855)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3046..3065
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3278..3300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3292..3309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3293..3309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3319..3338
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3319..3337
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3761..3772
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5868..6055
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5923..5947)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6130..7486
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        6130..6473
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6474..7283
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6541..6560)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      7289..7486
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7543..7563)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7543..7561)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7582..7598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7582..7598)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7595..7617)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7606..7622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7630..7636,7637..7654,7655..7660))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7675..7696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7813..7830)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7984..8572)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8064..8083)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8743..9534,9535..9603))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9366..9385
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9604..9708)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9776..9794
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttaaa cgttgcttta tatatggata acgacatata
      601 catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct gctttaaagt
      661 tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc aaaactcaaa
      721 taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat tcgttttttc
      781 aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca tcgaatatga
      841 gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg atggtagagg
      901 cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct cctgccaaac
      961 aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat gcaattgttg
     1021 aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac actgatgcct
     1081 tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg cgatgcattc
     1141 aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt cctgaatttt
     1201 gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt tatccttttg
     1261 tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt catcaaccat
     1321 ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt gaaaagttcg
     1381 ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga aaagtttatg
     1441 cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta tatgcccctg
     1501 ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat gcggtgcgca
     1561 cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat ggtgagacca
     1621 ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc attgaagctt
     1681 gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct ttcagcgaaa
     1741 tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt ttaggtactg
     1801 atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc ggtgcaactt
     1861 tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac gttaccatca
     1921 tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac gtcaaagatc
     1981 ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta agcaagatga
     2041 aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact attgtttcag
     2101 ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca agagggttgc
     2161 gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt gctgcaatga
     2221 cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga gttgactctc
     2281 ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct atcaataata
     2341 gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc tcccttttgg
     2401 ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag gctgataaat
     2461 tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata actgttaaat
     2521 gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga ttgtaaaatg
     2581 tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt gctgcgacgc
     2641 agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     2701 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2761 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2821 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2881 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2941 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3001 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3061 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3121 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     3181 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3301 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccgct
     3361 agcatgcgag ctttgcgtaa ttatgtacgg cgtcctggga tagtcgctgt ttgttaaaaa
     3421 aaaggtgatt cgcaactgat gtatgcagat ggcgatgaga aagtagagag aggtagtgaa
     3481 aaaagataat gaaatataga gaactacgta acgctccaca atagaacaaa taccgtgcac
     3541 taaagtttca agaaatttcc gcttcaatag gaaagaataa acaaattaga atggtttgtt
     3601 ggtacgggag aaagccgatt acgaacagtc aaggcaatga ttttgattga tatgatcaac
     3661 caattttgat tttactaatt caaaatgaaa tcctattaat ttcaatcgat agctcgcccc
     3721 ttttacttac aattttttga aagatgttgt tgggggatga aaagaggaga aatcgaaagc
     3781 aaacaactgg cacaatatag ccgcgaaaaa aaattttttt aatgtaaata taccgcgtta
     3841 cttgaccttc ctttataagt aaatttttag tcctgtgatc ttccgaggca gcatgtgccg
     3901 tttgcataat ttatgaccaa atccgttacc cacatatatc atttgtttgc cttgagcacc
     3961 ccggataaaa ggttgaagag ttggtttccc gatatatggt ccttacacta aagtaaagga
     4021 tgctcagtaa gcttctcccg tctagactgg aaatagcgca aaaatcattt ggtatgtata
     4081 atcaaatatt acggaacgga gtacgactta aattaattct attaacaaat tgaaagcgca
     4141 tagctctttc agatatgaaa gtgaagcaga aggtgagcat tattcgcact tagcaacaga
     4201 aaatttgtaa ctgacgacct tgcaaaggct tgtaaggtga gctaagttcc gcttccgtct
     4261 acggttctta ccgttcttct cctcgtttcc gtgcgatgcg gtaccgtgta aaggcatacc
     4321 ccgtcttttt ctataactat tttaacgaaa cttgttgaag cttaatcctc acgagttttc
     4381 cgtttgggta tttcgtgtgg tttcggatgt ttcaaaagtt tggggtaatc gacatttgtc
     4441 cgttccccag atttctgaag ataatgttaa ggcttaccga atcaataacc tgacgtgttc
     4501 attcgtacat gtgccattgt tgttgacaaa gaattaaccc catttacaag tatggtgcgg
     4561 acaaccaatt actatatttt taccaaggtg gtcctccaac aaacgaatcc gttcaacgac
     4621 ttgggtcacc ttatataggt tgatttgcaa gaagttttgc ttattccatc tttggacggt
     4681 tgtagcacat aagatgtcca tgttagtacc caaataaagt attttgtttt agtttagata
     4741 attatataaa attaaaacta acttatccaa cttaagcgtg tcagcaatgc aatcaagaac
     4801 tgctattatt tatttacacc ttgacgagta caccacttgt cattcatttt cgtaactttc
     4861 gggagctact ttgaggttgt tcctttcatt cttttttttt tataaattta gttcctttgt
     4921 tatattttat tttattgtaa tctaacttct gcatcttcta tcaaagggat atttcccatt
     4981 gtgctgagta cacaatgggc ctttgttagg tactctccat tgttgtccac catacaaaat
     5041 gaagggcttg tcctctcttc agacaccatt cggtgcggtt tctttggcaa agaatcactc
     5101 tttggatttt gttttttaga attcatggca agctccaagg gtgaagagct atttactggg
     5161 gttgtaccca ttttggtaga actggacgga gatgtaaacg gacataaatt ctctgttaga
     5221 ggtgagggcg aaggcgatgc caccaatggt aaattgactc tgaagtttat atgcactacg
     5281 ggtaaattac ctgttccttg gccaacccta gtaacaactt tgacatatgg tgttcaatgt
     5341 ttctcaagat acccagacca tatgaaaagg catgatttct ttaaaagtgc tatgccagaa
     5401 ggctacgtgc aagagagaac tatctccttt aaggatgacg gtacgtataa aacacgagca
     5461 gaagtgaaat tcgaagggga tacactagtt aatcgcatcg aattaaaggg tatagacttt
     5521 aaggaagatg gtaatattct cggccataaa cttgagtata atttcaactc gcataatgtg
     5581 tacattacag ctgacaaaca aaagaacgga attaaagcga attttaaaat caggcacaac
     5641 gtcgaagatg ggtctgttca acttgccgat cattatcagc aaaacacccc tattggtgat
     5701 ggtccagtct tgttacccga taatcactac ttaagcacac agtctagatt gtcaaaagat
     5761 ccgaatgaaa agcgtgatca catggtttta ttggaatttg tcaccgctgc aggaataact
     5821 cacggaatgg acgagcttta taagtaaggc gcgccacttc taaataagcg aatttcttat
     5881 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     5941 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     6001 ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat
     6061 ccgctaggga taacagggta atatagatct gtttagcttg cctcgtcccc gccgggtcac
     6121 ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca
     6181 ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt
     6241 gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag
     6301 acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc
     6361 ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct
     6421 tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca accatgggta
     6481 aggaaaagac tcacgtttcg aggccgcgat taaattccaa catggatgct gatttatatg
     6541 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg
     6601 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     6661 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     6721 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggcaaaa
     6781 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     6841 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     6901 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     6961 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc
     7021 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     7081 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     7141 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     7201 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     7261 tgatgctcga tgagtttttc taatcagtac tgacaataaa aagattcttg ttttcaagaa
     7321 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt
     7381 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa
     7441 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata
     7501 ctaacgccgc catccagttt aaacgagctc cagcttttgt tccctttagt gagggttaat
     7561 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7621 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7681 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7741 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7801 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7861 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7921 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7981 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8041 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8101 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8161 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8221 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8281 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8341 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8401 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8461 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8521 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8581 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8641 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8701 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8761 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8821 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8881 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8941 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9001 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9061 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9121 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9181 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9241 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9301 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9361 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9421 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9481 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9541 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9601 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9661 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9721 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     9781 gcgtatcacg aggccctttc gtc
//
