LOCUS       sequence-263753-        3355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a fluorecent protein with an RNA binding peptide: 
            mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter 
            (J23106), ribozyme PlmJ and RBS BBa_B0034..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Schwarz-Schilling M, Dupin A, Chizzolini F, Krishnan S, Mansy SS, 
            Simmel FC
  TITLE     Optimized Assembly of a Multifunctional RNA-Protein Nanostructure in
            a Cell-Free Gene Expression System.
  JOURNAL   Nano Lett. 2018 Apr 11;18(4):2650-2657. doi: 
            10.1021/acs.nanolett.8b00526. Epub 2018 Mar 27.
  PUBMED    29564885
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.nanolett.8b00526"; journalName: "Nano Lett"; date: 
            "2018-04-11- 11"; volume: "18"; issue: "4"; pages: "2650-2657"
COMMENT     Sequence Label: pSB1C3_mTq-Tat (c-plasmid)
FEATURES             Location/Qualifiers
     source          1..3355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     97..114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(138..195)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    196..216
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     promoter        367..401
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             498..509
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1526..1569
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1573..1591)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1753..2412
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1819..1838)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2433..2527
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2710..3298
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3199..3218
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
       61 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc
      121 tgcataacgc gaagtaatct tttcggtttt aaagaaaaag ggcagggtgg tgacaccttg
      181 cccttttttg ccggactgca gcggccgcta ctagtatgca cctcaggaca tctagtgatt
      241 atctcgactt ggattcggca caccaagttc cgaccgtgtg gacggtaaag ctacttcgtc
      301 caacgttgta tgatcaggct ggcatttcat aatccaagca aggcaccaat ctcgagattc
      361 aggatcttta cggctagctc agtcctaggt atagtgctag cgaagcttca gtcataagtc
      421 tgggctaagc ccactgatga gtcgctgaaa tgcgacgaaa cttatgacct ctacaaataa
      481 ttttgtttaa agtcagtaaa gaggagaaat agacatggtt agcaagggtg aagaactgtt
      541 aaccggcgtc gtgccgattc tggttgagct ggatggtgat gtcaacggtc acaagtttag
      601 cgttagcggt gagggcgagg gcgacgccac ctacggtaaa ttgaccctga agtttatctg
      661 cacgaccggt aagctgccgg ttccgtggcc gaccctggtg acgactctgt cgtggggcgt
      721 gcaatgtttc gcgcgctatc cggatcacat gaaacagcat gacttcttta agagcgcgat
      781 gccggaaggc tacgttcagg aacgtacgat ctttttcaaa gacgacggta actataagac
      841 ccgcgcagaa gtcaagttcg agggtgacac gctggtgaat cgtattgagc tgaaaggtat
      901 tgactttaaa gaggacggta acatcctggg tcacaaactg gagtataatt acttcagcga
      961 caatgtgtac atcaccgctg ataaacagaa aaacggcatt aaagcaaact tcaagatccg
     1021 tcacaatatt gaagatggcg gcgtgcaatt ggccgatcac tatcaacaga acaccccgat
     1081 tggcgatggt ccggtcctgc tgccagataa tcactacttg agcacgcaat ccaaactgtc
     1141 caaagatccg aacgaaaaac gtgaccacat ggtcctgctg gaatttgtta ccgcggcggg
     1201 tatcacgctg ggtatggacg aactgtacaa gacccgtggt ggcggcggca gcagcggtcc
     1261 gcgtcctcgt ggtacccgcg gtaagggtcg tcgcattcgt cgttaatggt actactttca
     1321 gccaaaaaac ttaagaccgc cggtcttgtc cactaccttg cagtaatgcg gtggacagga
     1381 tcggcggttt tcttttctct tctcaatcgg tgctctagaa gcggcttact cgaattcgag
     1441 taagccgctt ctagatgtcc tgaggtgcat actagtatgc acctcaggac atctagtgct
     1501 ctagaagcgg cttactcgaa ttccagaaat catccttagc gaaagctaag gatttttttt
     1561 atctgaaatt ctgcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     1621 tttcttagac gtcaggtggc actcgagttg atcgggcacg taagaggttc caactttcac
     1681 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1741 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     1801 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     1861 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     1921 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
     1981 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     2041 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     2101 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     2161 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     2221 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     2281 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     2341 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     2401 ggcggggcgt aatttgatat cgagctcgct tggactcctg ttgatagatc cagtaatgac
     2461 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
     2521 tgagaatcca agcctcgagc tgtcagacca agtttactca tatatacttt agattgattt
     2581 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2641 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2701 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2761 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2821 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2881 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2941 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3001 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3061 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3121 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3181 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3241 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tgtggaaaaa
     3301 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcac
//
