LOCUS       sequence-263758-        9185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A binary plasmid containing double CaMV 35S promoter and TEV 
            enhancer to drive bialophos resistance gene (bar) for plant 
            transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Paz, M.M., Shou, H., Guo, Z., Zhang, Z., Banerjee, A., Wang, K
  TITLE     Assessment of conditions affecting Agrobacterium-mediated soybean 
            transformation using the cotyledonary node explant
  JOURNAL   Euphytica (2004) 136: 167.
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Euphytica 
            (2004) 136: 167."
COMMENT     Sequence Label: pTF101.1
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    149..173
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1504..2133
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2562..3635
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3701..3895
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4131..4153)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4239..4379
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4294..4313
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4394..4411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4565..5153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4645..4664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5399..6190)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6711..6735
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6935..6957
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6949..6966
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6950..6966
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7536..8084)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(8236..8580)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8272..8292)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    8997..9053
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(9062..9078)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9062..9078)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9075..9097)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9086..9102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9110..9116,9117..9134,9135..9140))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9155..9176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
       61 caacgcgcgg ggagaggcgg tttgcgtatt ggagcttgag cttggatcag attgtcgttt
      121 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
      181 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
      241 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttaaagt
      301 actttaaagt actttaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct
      361 tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc
      421 gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa
      481 agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg
      541 gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg
      601 aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg
      661 accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga
      721 ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc
      781 aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg
      841 ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca
      901 tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc
      961 gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc
     1021 gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac
     1081 ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg
     1141 acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag
     1201 catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc
     1261 ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct
     1321 gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc
     1381 cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt
     1441 gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa
     1501 cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca
     1561 acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat
     1621 ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc
     1681 ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta
     1741 gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac
     1801 ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg
     1861 gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg
     1921 tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac
     1981 gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc
     2041 gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg
     2101 ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca
     2161 aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt
     2221 tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg
     2281 atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat
     2341 ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt
     2401 ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa
     2461 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg
     2521 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat
     2581 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg
     2641 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag
     2701 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga
     2761 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg
     2821 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg
     2881 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg
     2941 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc
     3001 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt
     3061 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga
     3121 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta
     3181 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc
     3241 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg
     3301 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg
     3361 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg
     3421 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg
     3481 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg
     3541 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca
     3601 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa
     3661 ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg
     3721 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca
     3781 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg
     3841 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt
     3901 ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc
     3961 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc
     4021 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc
     4081 gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     4141 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     4201 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     4261 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     4321 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     4381 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4441 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4501 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4561 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4621 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4681 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4741 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4801 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4861 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4921 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4981 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     5041 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5161 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5221 tggtcatgca tgatatatct cccaatttgt gtagggctta ttatgcacgc ttaaaaataa
     5281 taaaagcaga cttgacctga tagtttggct gtgagcaatt atgtgcttag tgcatctaac
     5341 gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa cgaatttcta gctagacatt
     5401 atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat
     5461 ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta
     5521 tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg
     5581 gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc
     5641 gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct
     5701 caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg
     5761 caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg
     5821 gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct
     5881 tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc
     5941 ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc
     6001 gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg
     6061 gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga
     6121 gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg
     6181 cttcccccat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc
     6241 tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag
     6301 gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     6361 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc
     6421 attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgcccga attgatcaca
     6481 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
     6541 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
     6601 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
     6661 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
     6721 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
     6781 atgtactgaa ttaacgccga attgctctag cattcgccat tcaggctgcg caactgttgg
     6841 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     6901 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     6961 gccagtgcca agctaattcg cttcaagacg tgctcaaatc actatttcca cacccctata
     7021 tttctattgc actccctttt aactgttttt tattacaaaa atgccctgga aaatgcactc
     7081 cctttttgtg tttgtttttt tgtgaaacga tgttgtcagg taatttattt gtcagtctac
     7141 tatggtggcc cattatatta atagcaactg tcggtccaat agacgacgtc gattttctgc
     7201 atttgtttaa ccacgtggat tttatgacat tttatattag ttaatttgta aaacctaccc
     7261 aattaaagac ctcatatgtt ctaaagacta atacttaatg ataacaattt tcttttagtg
     7321 aagaaaggga taattagtaa atatggaaca agggcagaag atttattaaa gccgcgtaag
     7381 agacaacaag taggtacgtg gagtgtctta ggtgacttac ccacataaca taaagtgaca
     7441 ttaacaaaca tagctaatgc tcctatttga atagtgcata tcagcatacc ttattacata
     7501 tagataggag caaactctag ctagattgtt gagcagatct cggtgacggg caggaccgga
     7561 cggggcggta ccggcaggct gaagtccagc tgccagaaac ccacgtcatg ccagttcccg
     7621 tgcttgaagc cggccgcccg cagcatgccg cggggggcat atccgagcgc ctcgtgcatg
     7681 cgcacgctcg ggtcgttggg cagcccgatg acagcgacca cgctcttgaa gccctgtgcc
     7741 tccagggact tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg
     7801 ggggagacgt acacggtcga ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg
     7861 cccgcgtagg cgatgccggc gacctcgccg tccacctcgg cgacgagcca gggatagcgc
     7921 tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc ggtacggaag
     7981 ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc agaccgccgg catgtccgcc
     8041 tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc tcatggtaga tcccccgttc
     8101 gtaaatggtg aaaattttca gaaaattgct tttgctttaa aagaaatgat ttaaattgct
     8161 gcaatagaag tagaatgctt gattgcttga gattcgtttg ttttgtatat gttgtgttga
     8221 gaattaattc tcgaggtcct ctccaaatga aatgaacttc cttatataga ggaagggtct
     8281 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc
     8341 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
     8401 ggtccatctt tgggaccact gtcggcagag gcatcttcaa cgatggcctt tcctttatcg
     8461 caatgatggc atttgtagga gccaccttcc ttttccacta tcttcacaat aaagtgacag
     8521 atagctgggc aatggaatcc gaggaggttt ccggatatta ccctttgttg aaaagtctca
     8581 attgcccttt ggtcttctga gactgtatct ttgatatttt tggagtagac aagtgtgtcg
     8641 tgctccacca tgttatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
     8701 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
     8761 tcttcaacga tggcctttcc tttatcgcaa tgatggcatt tgtaggagcc accttccttt
     8821 tccactatct tcacaataaa gtgacagata gctgggcaat ggaatccgag gaggtttccg
     8881 gatattaccc tttgttgaaa agtctcaatt gccctttggt cttctgagac tgtatctttg
     8941 atatttttgg agtagacaag tgtgtcgtgc tccaccatgt tgacctgcag gcatgcaagc
     9001 ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa ttcgtaatca
     9061 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9121 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9181 cgttg
//
