LOCUS       sequence-263759-        9493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mTagBFP2 tagged CHD domain to monitor actin 
            cytoskeleton.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 9493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0771
FEATURES             Location/Qualifiers
     source          1..9493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2697..3152)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2834..2855)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3046..3065
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3278..3300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3292..3309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3293..3309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3319..3338
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3319..3337
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4230..4940
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             4947..5657
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5664..6374
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      6959..7208
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6975..6993)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(7233..7253)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7233..7251)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7272..7288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7272..7288)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7285..7307)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7296..7312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7320..7326,7327..7344,7345..7350))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7365..7386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7503..7520)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7674..8262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7754..7773)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8433..9224,9225..9293))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9056..9075
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9294..9398)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9466..9484
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttaaa cgttgcttta tatatggata acgacatata
      601 catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct gctttaaagt
      661 tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc aaaactcaaa
      721 taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat tcgttttttc
      781 aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca tcgaatatga
      841 gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg atggtagagg
      901 cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct cctgccaaac
      961 aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat gcaattgttg
     1021 aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac actgatgcct
     1081 tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg cgatgcattc
     1141 aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt cctgaatttt
     1201 gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt tatccttttg
     1261 tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt catcaaccat
     1321 ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt gaaaagttcg
     1381 ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga aaagtttatg
     1441 cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta tatgcccctg
     1501 ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat gcggtgcgca
     1561 cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat ggtgagacca
     1621 ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc attgaagctt
     1681 gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct ttcagcgaaa
     1741 tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt ttaggtactg
     1801 atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc ggtgcaactt
     1861 tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac gttaccatca
     1921 tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac gtcaaagatc
     1981 ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta agcaagatga
     2041 aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact attgtttcag
     2101 ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca agagggttgc
     2161 gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt gctgcaatga
     2221 cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga gttgactctc
     2281 ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct atcaataata
     2341 gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc tcccttttgg
     2401 ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag gctgataaat
     2461 tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata actgttaaat
     2521 gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga ttgtaaaatg
     2581 tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt gctgcgacgc
     2641 agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     2701 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2761 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2821 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2881 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2941 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3001 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3061 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3121 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     3181 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3301 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccatt
     3361 taaatgatct acgataatga gacggtgttt gggttgtttt tactgaattt ttcttttatt
     3421 ttatgacatc ttaattttta tgttaatgat tattcttata cttttatcta tctttgatta
     3481 aaactaatat tcattctttt gatcactaat tatttttata gtatacgatt caacccaact
     3541 ataatacatt gtatcaagag aaatttttaa ttagatgtaa tttaatagcc atttgataaa
     3601 aaaaaattac cttttttttt gctagtgtta aattattgat cataacaatg tcgaatgaat
     3661 actaataaaa tagggacacg cgagttgcaa atacttgaaa tacctttgaa gatttgtctg
     3721 tgaattttga taagattttt atgaccatct cttttatatt cgatatcgtg aactttcatt
     3781 ttcttttgtt ttttgctttt caagttaaat aacgcggaaa ttatatttcc ttatatgttt
     3841 ataagagctt ttaaaggatt tgctatatcg cttacagtac tacaaccggt tgctcaatgt
     3901 tatccgtttc cgcggtcttc ttccgtgcgc attctgccgt gaagtgctaa cgctgtgtgt
     3961 ggctttatca aaaaagtggc tcaaatatag caatttcata catatttaaa cacccattat
     4021 acttaaatgt atattttaaa atatataaga gattttcact gtataactgt gtatcttatc
     4081 tatatgtaat caacggcttc ataccacctc agccagccgt gttataactt accgtttacc
     4141 aactacattt tttgtaacga accaaaaaac cctcaaaaga caagaccctc gaggtcgacg
     4201 aattcttaat taacaattct agaactagta tggtgtctaa gggcgaagag ctgattaagg
     4261 agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca
     4321 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg
     4381 agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca
     4441 agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg
     4501 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
     4561 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca
     4621 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc
     4681 tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg
     4741 ggagccatct gatcgcaaac gccaagacca catatagatc caagaaaccc gctaagaacc
     4801 tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca
     4861 acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac ctccctagca
     4921 aactggggca caagcttaat gctagtatgg tgtctaaggg cgaagagctg attaaggaga
     4981 acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat
     5041 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
     5101 gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga
     5161 ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct
     5221 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     5281 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat
     5341 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt
     5401 accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga
     5461 gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca
     5521 agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg
     5581 agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac
     5641 tggggcacaa gcttaatgct agtatggtgt ctaagggcga agagctgatt aaggagaaca
     5701 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     5761 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     5821 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     5881 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     5941 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     6001 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     6061 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
     6121 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     6181 atctgatcgc aaacgccaag accacatata gatccaagaa acccgctaag aacctcaaga
     6241 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     6301 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     6361 ggcacaagct taatgctagc atggacgtaa atgtgggatt atctagactt caatctcaag
     6421 ctggggcacc tgttggcacg aaagggtcga acactcgttt agcagccaaa caaagagaaa
     6481 cccttcaagc atatgattat ttgtgtaggg ttgatgaagc aaaaaaatgg atagaagaat
     6541 gcctcggcac agatctagga ccaacgtcta catttgagca gtcgctgcga aacggagttg
     6601 tcttggctct cttggttcag aaatttcaac ccgataaact aatcaagata ttctattcta
     6661 atgaactcca gtttcgccat tcagataaca ttaataagtt tcttgacttt atccatggca
     6721 ttgggcttcc cgaaattttt catttcgaac tgactgacat ttacgaaggg aaaaatttac
     6781 ccaaagttat ctattgtatt catgcccttt cttatttcct ttctatgcag gacttggcac
     6841 cccctttaat caagagtgat gaaaatctat cttttactga tgaagacgtt tcaataatag
     6901 tccgtagact ccgacaatct aatgtaatcc ttcctaactt caaagcttga ggcgcgcctc
     6961 atgtaattag ttatgtcacg cttacattca cgccctcctc ccacatccgc tctaaccgaa
     7021 aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttta atagttatgt
     7081 tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac aaacgcgtgt
     7141 acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt
     7201 taatttgcag atctgagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     7261 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7321 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7381 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7441 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7501 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7561 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7621 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7681 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7741 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7801 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7861 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7921 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7981 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8041 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8101 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8161 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8221 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8281 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8341 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8401 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8461 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8521 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8581 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8641 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8701 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8761 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8821 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8881 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8941 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9001 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9061 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9121 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9181 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9241 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9301 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9361 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9421 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     9481 aggccctttc gtc
//
