LOCUS       sequence-263772-       10286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of 3GFP tagged CRIB domain to monitor active Cdc42.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0757
FEATURES             Location/Qualifiers
     source          1..10286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1178..1828
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQK
                     GEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQA
                     MGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLYS
                     FSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     rep_origin      complement(2262..2717)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2399..2420)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2611..2630
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2843..2865
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2857..2874
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2858..2874
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2884..2903
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2884..2902
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4153..4866
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             4873..5586
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             5593..6309
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      6351..6538
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6406..6430)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6613..7969
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        6613..6956
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6957..7766
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7024..7043)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      7772..7969
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8026..8046)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8026..8044)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8065..8081)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8065..8081)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8078..8100)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8089..8105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8113..8119,8120..8137,8138..8143))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8158..8179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8296..8313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8467..9055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8547..8566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9226..10017,10018..10086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9849..9868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10087..10191)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10259..10277
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggatggaacg attgaatact gtgctactct ttatgtaata taacgaattc
      241 aactgttcaa cgtatgtgac tgctctctgc ttctcggtat atatttactt gaaggaatta
      301 atttttcttt gccaatggtc tcttttacca attgcagtct ggcttaaagc aatgaggttg
      361 taattaaatg gcttgagacc tagaacaaac accttttaca atccttgttt actggttttg
      421 atagtaaatt cggttcgctt gtggtgtaca ttatattttt taggctagtc gtgatttttt
      481 gataatatca atattcttag atttgagaca ttcatgcttt ttaatttgat caatcgtttg
      541 tatcttcatt aattagtagg tggtttgata gaacatttag ttgcttgttt gactacaaat
      601 atctgcagaa tggattcggt taataaaaaa aatgtttgag gaattaattt tcgctttgga
      661 gtggaggcct caattcgatg ttgcagattt tacaagtttt taaaatgtat ttcattatta
      721 ctttttatat gcctaataaa aaagccatag tttaatctat agataacttt ttttccagtg
      781 cactaacgga cgttacattc ccatacaaaa ctgcgtagtt aaagctaagg aaaagttaat
      841 atcatgttaa ttaaatacgc tatttacaat aagacattga actcatttat atcgttgaat
      901 atgaataacc aatttcagcg aatttttaac aaacatcgtt cacctcgttt aaggatatct
      961 tgtgtatggg gtgttgactt gctttatcga ataattaccg tacctgtaat tggcttgctg
     1021 gatatagcgg tagtctaata tctagcaaaa atcttttggg tgaaaaggct tgcaatttca
     1081 cgacaccgaa ctatttgtca ttttttaata aggaagtttt ccataaattc ctgtaattct
     1141 cggttgatct aattgaaaag agtagttttg catcacgatg aggagggctt ttgtagaaag
     1201 aaatacgaac gaaacgaaaa tcagcgttgc catcgctttg gacaaagctc ccttacctga
     1261 agagtcgaat tttattgatg aacttataac ttccaagcat gcaaaccaaa agggagaaca
     1321 agtaatccaa gtagacacgg gaattggatt cttggatcac atgtatcatg cactggctaa
     1381 acatgcaggc tggagcttac gactttactc aagaggtgat ttaatcatcg atgatcatca
     1441 cactgcagaa gatactgcta ttgcacttgg tattgcattc aagcaggcta tgggtaactt
     1501 tgccggcgtt aaaagatttg gacatgctta ttgtccactt gacgaagctc tttctagaag
     1561 cgtagttgac ttgtcgggac ggccctatgc tgttatcgat ttgggattaa agcgtgaaaa
     1621 ggttggggaa ttgtcctgtg aaatgatccc tcacttacta tattcctttt cggtagcagc
     1681 tggaattact ttgcatgtta cctgcttata tggtagtaat gaccatcatc gtgctgaaag
     1741 cgcttttaaa tctctggctg ttgccatgcg cgcggctact agtcttactg gaagttctga
     1801 agtcccaagc acgaagggag tgttgtaaag atgaattgga ttatgtcagg aaaagaacga
     1861 caattttgca tccaaattgt ctaaatttta gagttgcttg aaaacaatag aaccttactt
     1921 gctttataat tacgttaatt agaagcgtta tctcgtgaag gaatatagta cgtagccgta
     1981 taaattgaat tgaatgttca gcttatagaa tagagacact ttgctgttca atgcgtcgtc
     2041 acttaccata ctcactttat tatacgactt taagtataaa ctccgcggtt atggtaaaat
     2101 taatgatgca caaacgtccg attccatatg ggtacactac aattaaatac ttttaagtaa
     2161 cgctgatttc ttatttttta tccttggtgt atttattgtt tataccgcta gggcggtgtg
     2221 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
     2281 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     2341 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     2401 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     2461 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     2521 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     2581 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     2641 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     2701 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     2761 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     2821 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
     2881 gcgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga ggtcgactca
     2941 aattcactga tttaagactt tataacaaaa agatcttttt tataaatcct aactgattag
     3001 tgaaattaat ataagataat atgcttgctg gtgaatattg aatcaaaact tgctactgaa
     3061 gtgatagtag cattcaaccc tacgcttaga acttgatctt caccaacaac ccagtaacct
     3121 actttatact tctttatgcg ccagcgagta taccaccgaa gtcttataag ttttgtttaa
     3181 tttagaacta tacattaaac tatcaagaaa attatccaag taaacagtgc tgacgcgacc
     3241 ttcgtgttct agtttatcat ttatagagat ttagtcaatt gagtagattc tttgatattc
     3301 ttttcttttt gaaaaggatc tatttcaaaa gtttcctttt cggttgttaa tctgtacaac
     3361 tgattcctca taaaagtggc agcggcacaa gttactgtgc cctttgcgcc tcttactagg
     3421 agaaaaatcc ttttcaactt aagtgctcta ttcttgtctt tttgtttttt ttttgcatac
     3481 tgaaacttat tccttcgttc tgcaacacat ttgccatagt cctatttctg tttttcttta
     3541 cccttttcgt taataaattt atttactatg ggaggtcccg ggactagtgc aagtattacc
     3601 aatactggaa acgaaaccat gaaccttcca cagatgcggt cgatttggct ggatgaagat
     3661 gaagaagctg aaaaactcta cggtctgcag gcccagcaat tcatgggatc tgatgatgaa
     3721 gaaaacctag gcattacttt catcaacagc gataaacctg tgctgagtaa caagaaaaac
     3781 attgagttgc ctccactttc accaaattca catccgtctt gccaccacag gagaagtaat
     3841 tctaactctg caaagtctaa agaatcatcg tcatcatcgt ccagcgccaa caagacaaat
     3901 cacaaaaagg ttttccttaa gcttaatttg ttgaagaaaa agttgcttgg tgcccaaccg
     3961 gacataagag gtaaaggtat ctccacacca tttgattttc aacatatttc acatgctgac
     4021 actagaaatg gattccaaga tgagcaattg caggaacctt catcgctgtc cacagagatt
     4081 aaggacgact atacctcctc ctcaagcaag cgggattcga aattaattaa cggaacaaaa
     4141 gctggaggat ccatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
     4201 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
     4261 gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca
     4321 tggccaacct tagtcactac tttcggttat ggtgttcaat gttttgcgag atacccagat
     4381 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
     4441 actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt
     4501 gataccttag ttaatagaat cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt
     4561 ttaggtcaca aattggaata caactataac tctcacaatg tttacatcat ggctgacaaa
     4621 caaaagaatg gtatcaaagt taacttcaaa attagacaca acattgaaga tggttctgtt
     4681 caattagctg accattatca acaaaatact ccaattggtg atggtccagt cttgttacca
     4741 gacaaccatt acttatccac tcaatctgcc ttatccaaag atccaaacga aaagagagac
     4801 cacatggtct tgttagaatt tgttactgct gctggtatta cccatggtat ggatgaattg
     4861 tacaaaagat ccatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
     4921 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
     4981 gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca
     5041 tggccaacct tagtcactac tttcggttat ggtgttcaat gttttgcgag atacccagat
     5101 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
     5161 actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt
     5221 gataccttag ttaatagaat cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt
     5281 ttaggtcaca aattggaata caactataac tctcacaatg tttacatcat ggctgacaaa
     5341 caaaagaatg gtatcaaagt taacttcaaa attagacaca acattgaaga tggttctgtt
     5401 caattagctg accattatca acaaaatact ccaattggtg atggtccagt cttgttacca
     5461 gacaaccatt acttatccac tcaatctgcc ttatccaaag atccaaacga aaagagagac
     5521 cacatggtct tgttagaatt tgttactgct gctggtatta cccatggtat ggatgaattg
     5581 tacaaaagat ccatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
     5641 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
     5701 gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca
     5761 tggccaacct tagtcactac tttcggttat ggtgttcaat gttttgcgag atacccagat
     5821 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
     5881 actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt
     5941 gataccttag ttaatagaat cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt
     6001 ttaggtcaca aattggaata caactataac tctcacaatg tttacatcat ggctgacaaa
     6061 caaaagaatg gtatcaaagt taacttcaaa attagacaca acattgaaga tggttctgtt
     6121 caattagctg accattatca acaaaatact ccaattggtg atggtccagt cttgttacca
     6181 gacaaccatt acttatccac tcaatctgcc ttatccaaag atccaaacga aaagagagac
     6241 cacatggtct tgttagaatt tgttactgct gctggtatta cccatggtat ggatgaattg
     6301 tacaaataaa gatctgtatt ctttaatatc ggcgcgccac ttctaaataa gcgaatttct
     6361 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     6421 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     6481 tcaggttgct ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca
     6541 gatccgctag ggataacagg gtaatataga tctgtttagc ttgcctcgtc cccgccgggt
     6601 cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc
     6661 tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca
     6721 tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg
     6781 cagacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg
     6841 cccctgtaga gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt
     6901 ccttttaaaa tcttgctagg atacagttct cacatcacat ccgaacataa acaaccatgg
     6961 gtaaggaaaa gactcacgtt tcgaggccgc gattaaattc caacatggat gctgatttat
     7021 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
     7081 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     7141 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     7201 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggca
     7261 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     7321 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     7381 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     7441 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     7501 agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     7561 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     7621 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     7681 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     7741 atttgatgct cgatgagttt ttctaatcag tactgacaat aaaaagattc ttgttttcaa
     7801 gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag
     7861 cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca
     7921 gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg
     7981 atactaacgc cgccatccag tttaaacgag ctccagcttt tgttcccttt agtgagggtt
     8041 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8101 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8161 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8221 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8281 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8341 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8401 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8461 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8521 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8581 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8641 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8701 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8761 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8821 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8881 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8941 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9001 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9061 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9121 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9181 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9241 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9301 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9361 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9421 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9481 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9541 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9601 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9661 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9721 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9781 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9841 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9901 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9961 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10021 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10081 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10141 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10201 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10261 taggcgtatc acgaggccct ttcgtc
//
