LOCUS       sequence-263775-       10586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of sfGFP tagged beta-tubulin to monitor microtubules.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 10586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0772
FEATURES             Location/Qualifiers
     source          1..10586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2697..3152)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2834..2855)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3046..3065
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3278..3300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3292..3309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3293..3309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3319..3338
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3319..3337
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            6689..8264
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        6689..7032
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7033..8061
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMRLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVGASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      8067..8264
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8326..8346)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8326..8344)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8365..8381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8365..8381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8378..8400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8389..8405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8413..8419,8420..8437,8438..8443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8458..8479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8596..8613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8767..9355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8847..8866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9526..10317,10318..10386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10149..10168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10387..10491)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10559..10577
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttaaa cgttgcttta tatatggata acgacatata
      601 catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct gctttaaagt
      661 tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc aaaactcaaa
      721 taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat tcgttttttc
      781 aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca tcgaatatga
      841 gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg atggtagagg
      901 cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct cctgccaaac
      961 aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat gcaattgttg
     1021 aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac actgatgcct
     1081 tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg cgatgcattc
     1141 aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt cctgaatttt
     1201 gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt tatccttttg
     1261 tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt catcaaccat
     1321 ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt gaaaagttcg
     1381 ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga aaagtttatg
     1441 cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta tatgcccctg
     1501 ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat gcggtgcgca
     1561 cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat ggtgagacca
     1621 ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc attgaagctt
     1681 gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct ttcagcgaaa
     1741 tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt ttaggtactg
     1801 atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc ggtgcaactt
     1861 tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac gttaccatca
     1921 tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac gtcaaagatc
     1981 ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta agcaagatga
     2041 aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact attgtttcag
     2101 ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca agagggttgc
     2161 gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt gctgcaatga
     2221 cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga gttgactctc
     2281 ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct atcaataata
     2341 gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc tcccttttgg
     2401 ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag gctgataaat
     2461 tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata actgttaaat
     2521 gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga ttgtaaaatg
     2581 tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt gctgcgacgc
     2641 agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     2701 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2761 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2821 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2881 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2941 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3001 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3061 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3121 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     3181 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3301 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccaaa
     3361 taaatcaaaa ctttttccgc gtaactctag tataagccgg aacaaggcta agaaacaaat
     3421 aaatgcaacg tttttatatt tcgatctatc aaatcctcaa ttacggaagg gcaattagta
     3481 gcgacacaag agatatacgc tgctgacaag tttttcgtcg taataaaaga gcttttgaag
     3541 agaaaaaaat tacggcggag cgttgcaatt cgttatatat aatgtgctat gcataaactc
     3601 tacttttgct aggaattctc cttgccctta tgtaatattt tatatacttt taaaaagatt
     3661 tacatgagga tatacctgtt ctataaagga gcacatgttg agttttaatg gaaatttacc
     3721 aatccaatct ttcacttaca aattgagaaa ataaaaggtg aaattgatga agatcctaat
     3781 ctttttatga ttagaaagct taaaacacct gactgagtaa gcttcttttt gcaagcaatg
     3841 cattatttta atgtctattt acatgtttag catacttcct tccgtatcat caataaatat
     3901 cgttagactt ctttattaag gaattgtttt gattaagtgt acaccgcact tcactacaaa
     3961 ccaaacacca aaaacctcgt agaaaccctg gaaagacgtt cgaattaatt aaaatgtcca
     4021 agggtgaaga gctatttact ggggttgtac ccattttggt agaactggac ggagatgtaa
     4081 acggacataa attctctgtt agaggtgagg gcgaaggcga tgccaccaat ggtaaattga
     4141 ctctgaagtt tatatgcact acgggtaaat tacctgttcc ttggccaacc ctagtaacaa
     4201 ctttgacata tggtgttcaa tgtttctcaa gatacccaga ccatatgaaa aggcatgatt
     4261 tctttaaaag tgctatgcca gaaggctacg tgcaagagag aactatctcc tttaaggatg
     4321 acggtacgta taaaacacga gcagaagtga aattcgaagg ggatacacta gttaatcgca
     4381 tcgaattaaa gggtatagac tttaaggaag atggtaatat tctcggccat aaacttgagt
     4441 ataatttcaa ctcgcataat gtgtacatta cagctgacaa acaaaagaac ggaattaaag
     4501 cgaattttaa aatcaggcac aacgtcgaag atgggtctgt tcaacttgcc gatcattatc
     4561 agcaaaacac ccctattggt gatggtccag tcttgttacc cgataatcac tacttaagca
     4621 cacagtctag attgtcaaaa gatccgaatg aaaagcgtga tcacatggtt ttattggaat
     4681 ttgtcaccgc tgcaggaata actcacggaa tggacgagct ttataaggtc gacatgagag
     4741 agatcatttc cattcacgtt ggccaagcag gaactcaaat cggaaatgct tgttgggaac
     4801 tgtattgttt agaacatggt attcaaccaa atggttacat gaatcctgag actgcatccc
     4861 aaaatagtga tggcgggttt tctacttttt tctcagaaac tggtcaagga aaatacgttc
     4921 ctagaagtat ttatgtagat cttgaaccga atgtgatcga ccaagttcgt actggcccat
     4981 accgtgatct tttccatcca gagcaattaa ttacgggcaa ggaagatgca tcaaacaatt
     5041 atgctcgtgg tcactacact gttggaaaag agctggttga tgaagtaact gacaagattc
     5101 gtcgtattgc cgataattgc tccggccttc agggctttct cgtcttccat tcgtttggag
     5161 gtggtaccgg ttcaggcttt ggtgcccttt tacttgaacg cttggccatg gaatacacga
     5221 aaaaatcaaa gcttcaattt tctgtgtatc cggctcctca agttagtact tctgtagttg
     5281 aaccttataa ctccgttttg actacccatg ctacgttgga ccttgctgac tgtacattta
     5341 tggttgacaa cgaatcgtgc tatgatattt gccgtagaaa cttagatatt gagcgtcctt
     5401 cctatgagaa tctcaataga ttgatcgctc aggttgtttc ttccatcacc gcctctcttc
     5461 gcttcgaggg cagcttgaat gttgatttgg cagaattcca aacgaatttg gtaccttatc
     5521 ctcgtattca tttccctctc gtcacttatg caccaattgt tagtgccgcc aaggccttcc
     5581 atgaatctaa cagcgttcaa gaaatcacca atcaatgttt tgaaccctac aaccaaatgg
     5641 ttaaatgtga tcctcgtgcc ggtcgctaca tggcaacgtg tttgctgtat cgtggtgatg
     5701 ttatccctcg cgatgttcaa gctgctgtaa cgaccattaa ggccaagcgt acaattcagt
     5761 tcgttgactg gtgtcctact ggattcaaaa tcggtatttg tgatcgtcca ccccaacaca
     5821 ttgaaggtag tgaaattgct aaggttgacc gtgccgtctg tatgctctcc aatactacct
     5881 ctattgcgga ggcttggtca aggctcgatc acaaatttga tctcatgtat agcaagcgtg
     5941 cttttgttca ttggtatgtt ggtgaaggta tggaagaagg cgagttttct gaagctcgtg
     6001 aggatcttgc tgctcttgaa cgcgattatg aggaagttgg tcaagattcg atggaagtcg
     6061 attacatgga agaagagtac taaagaattt cgtttatatg ctttaaagat tttcatttaa
     6121 taaccttctt ttatacgtat tatatttttt ttttactttt tctcaatctc tctcacactc
     6181 tctacatgac atatacaaaa ttgctaatac tacttttctt ttaccttaca aatgttgcaa
     6241 tcggtgaacg ctgtcaaagt ttattcacaa taactagaag tataaatttt cttttgacat
     6301 tcccatgcaa ctatgcattc cattccttta cgtgtctttc tttctggttg catcgtcatt
     6361 agattaaact tgtgctatta tggttgcaac cacttgttta ctgcactctt ttttggttct
     6421 acagctttcc ccgattagtt tacattttca atacccatat ttttttatcc tattagatat
     6481 ccgtaaactg ttttgaaact aatttctctt ccgagtcgtg ttaagaatag aaatagccgt
     6541 ttaacacttg cgtaagcata tactttctgt ttgtaaagct tggggatatt taattggaat
     6601 tttttgtata taataattgt gagattgcta aacgaaataa tgtgagctcg tttagcttgc
     6661 ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag
     6721 tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca
     6781 tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa
     6841 ttacggctcc tcgctgcgga cctgcgagca gggaaacgct cccctcacag acgcgttgaa
     6901 ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt
     6961 cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga
     7021 acataaacaa ccatgggtaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     7081 atcgaaaagt tcgacagcgt ctccgacctg atgcggctct cggagggcga agaatctcgt
     7141 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     7201 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     7261 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     7321 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     7381 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     7441 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     7501 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcggtgc gtccgtcgcg
     7561 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     7621 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     7681 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     7741 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     7801 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     7861 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     7921 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     7981 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     8041 cgtccgaggg caaaggaata atcagtactg acaataaaaa gattcttgtt ttcaagaact
     8101 tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga
     8161 tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag
     8221 taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact
     8281 aacgccgcca tccacctgca ggtaatcacg tgccagcttt tgttcccttt agtgagggtt
     8341 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8401 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8461 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8521 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8581 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8641 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8701 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8761 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8821 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8881 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8941 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9001 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9061 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9181 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9361 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9421 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9481 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10441 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10501 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10561 taggcgtatc acgaggccct ttcgtc
//
