LOCUS       sequence-263784-        9758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nucleo-cytoplasmic mCherry in P-cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 9758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0543
FEATURES             Location/Qualifiers
     source          1..9758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2526..2981)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2663..2684)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2875..2894
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3107..3129
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3121..3138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3122..3138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3148..3167
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3148..3166
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3191..3207
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3192..3208
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5329..6039
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5474..5492)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5742..5761
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      6060..6247
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6115..6139)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6322..7441
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        6322..6665
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6666..7238
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      7244..7441
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7498..7518)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7498..7516)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7537..7553)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7537..7553)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7550..7572)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7561..7577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7585..7591,7592..7609,7610..7615))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7630..7651
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7768..7785)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7939..8527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8019..8038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8698..9489,9490..9558))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9321..9340
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9559..9663)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9731..9749
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg gggccttacg aagttaaatt ataggcacag acatctttgg cattggaaag
      241 atttagtaac aatcataatg tagcacttct taaaaaatct aattttagaa caaaaaggct
      301 taaaaaagaa tggttccgct cttttaaaaa atagtaaaat gtacaatagc aaggttttcg
      361 taggtcgacg gacataacaa ggggaaagag aaataaagca tgtaaacgtc aataaaacat
      421 ctttgattct ggatcaatca tatgccaaat gaagaaaaat agcatttata acaacatgaa
      481 accatatttg agacctttta acgatgaaaa tttattaaat aaaagataaa aaagaaagaa
      541 accgttcaag ttcaatgaaa atttcgtcgt gaagtagaaa ccaagataat ggaaaggctt
      601 cgcaaagcca caaattttcc aatagtcatt ataactgaag attaaggaca gtatactagt
      661 aaaaatcaag tgaattgtgc ctaggatagg tagctaacgt gactgccact gaacctgtgt
      721 agcgtattag atccatcaag atcccttctg ctcatatcat tgctgatctg taaattgacc
      781 gcatcaaatt tgatctcatt tatatcgaga ttgatctata ttattttgcc aatttgcagt
      841 atataaactt atttgattaa gtttttggtc tccttcgcca cgtcttcatt ttgttagttt
      901 aaattttttt gactaactta gaaagggaag tttttaaaaa cgttgaaata ctttttcgat
      961 ttaaaaatat atatttaatt aattttctgt tccaaaatgg ttgctcctca tctttggtta
     1021 cgtgctgaaa ccaaaccttt agaagagcgc agtgctttga ctcctagaac tgccaagata
     1081 ctcgccgatg ctggatttca aattaccatt gagcgctctt cccaacgtgc tttcaaggac
     1141 aaagaatttg agcgccttgg tttccccatg gttcctgagg gctcatggcg acatgcacct
     1201 aaggacgcgt acattattgg tctcaaagag cttccggaaa atgataacag tcctttgaag
     1261 catactcata ttcaatttgc tcattgctac aaaaaccagg aaggttggcg tgaagtcttg
     1321 tcccgattcc ccgctggcaa tggtcttcta tatgatttgg aatttttgca agatgacaat
     1381 ggcagacgtg ttgccgcttt tggttaccac gctggttttg ctggatcagc tatctcatgt
     1441 ttagtttggg cacatcaatt attgcatcct aacaaacaat tccctgctat ccgtccgttc
     1501 cccaacgaaa aatctttggt tcgccatgtt gctcgtcaag tacgtttagc tttgaaaaag
     1561 aataacaacc agtatcctcg tattctggtt attggggctc ttggtcgttg tggtactgga
     1621 gcgtgcgatt tagcatcaaa gattggtatt cccttcgata acattcttcg ctgggacatt
     1681 aatgaaacta agaagggtgg tccctttacc gaaattacgg aatcagacat atttgttaac
     1741 tgcatttatc tgagcatgcc tattcccaaa ttctgtaccg tcgagtcgtt aaatgtacct
     1801 aaccgtaaat tgcgtgttgt ttgtgatgtt agctgtgaca ctaccaatcc taacaatcct
     1861 attccaattt ataatgtaaa cactacattt gatcatccta cagttgaggt taaaggtgtc
     1921 acaactcctc ctcctttaga ggtcatttct attgatcatc ttcctacttt attgccaagg
     1981 gaatcttccg aagcatttag tgaagctcta attcctagtc ttttggcttt gaaggacgtc
     2041 gacaatgctc ccgtttgggt cagagctaaa aaactttatg aaaccatggt tcaaaagctt
     2101 taaaataaat gttatttaat ttcaaagttc cgggtccttt atattgtttc accaaattat
     2161 tgaatattat tttctcaaaa attaacttta gaatacaatt tagacggcat tttcgatgct
     2221 ctggacattt tttccacgta cttgttaaat tgtagcggtg ttgcataata ctaccttttg
     2281 atttgttatt cacgttacaa tatatatttg actaatttaa atatgataat atacacattt
     2341 ctctgcaaac ctcctttata cttttcgccg gtcattgatt aataattaag aaacaggatt
     2401 tcatcctgca tttgccactt acctatatgg taaatataaa tcttgaaact ttccccaata
     2461 tttattgctt gctagggcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2521 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2581 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2641 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2701 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     2761 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     2821 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     2881 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     2941 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg
     3001 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     3061 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     3121 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc
     3181 gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagaagcat
     3241 gcacgctgct cacttacaag ataacctttt gttgaaaagg aagatttatt aactatatat
     3301 gattgaagat tttttctttt caaaggatat ataagttctg caaagtttcc taaaaaaatc
     3361 tttctttttt gtttcttaca ccttttgaca tttatattgg agcctaacgt tttgccgctt
     3421 tgatatcatt tatccgttga ttttagaaaa aaaagttagg aagagttgtt accgattttt
     3481 tccttctttt tatgccaata ttcacttcat cagatttaat ttggctactt tttttttcgt
     3541 aacctactat agttatctca ttatagaatg aaatgaaact ctattattat gtagttaaaa
     3601 tcaatgataa gcactgtatt aaagtttaag agaaaatcgc gaattccttt tgatgtcctt
     3661 ttctcaaagg atacaaacac gatttataac acctttgctt cagacgtttg attgatatgc
     3721 ctattgtgat atacgaaaca tcagcatcag gttatagccg tgcaacattt tctgataaat
     3781 gatgaggaaa acagatgcta ttgtcttgct catgttggtt ctctgggttg agataatgaa
     3841 catattggta tataaaactt gcacacggat caacgagaat tgcttacttt tattgctctt
     3901 acattttatt gcttttattt tatataacat tgattgctct cttcttttct tgtactaaaa
     3961 gaaatacaaa catacaaata tggcttacat gttttacttc gcagaattat aattaattaa
     4021 atacccaagt tattcgttag catggttgag tatattgttt aataatagcg ctatttaagt
     4081 cgtaacttta taggataagt attaccagta ctaatacgtg taagaagtta cctagtgcta
     4141 ttgttaacaa aatataagct cgttaatgtg agtaacttgt tttattttct acttgaaatt
     4201 tttaacaaac aaaccagtta aaaaaaattg atctggatca atactccagc tatttggaaa
     4261 gaattgactt acgcgtaaat gtgtacgcgt taatgtttac tgatttcgtt ttcgggttta
     4321 gagaagtaaa ggatggaatt agattacagg aaagagtatt tttataaatt atatataaag
     4381 gaagatatga ctaacggcta gtgtaccaat tccataatat accgagtgca attcaaacca
     4441 aactctcgta tataggctgc gatttataaa gtaaaacttc agcgcatcag cacaattgtt
     4501 cacgagtaca aaatctaaaa agaaattttc tttgcaatgc gctcatcaat taatataaac
     4561 gaccgcgata tatgaagtca aacagtcgtt aaacaccaaa atttctttac gtatttactt
     4621 cgcagattac ccatcaaaaa gatattctat ggatttatta aagataaaga attcgctttt
     4681 gatgcctcaa attggaatac tatgctttag ataatcaaat gggctgaact ctttgaccga
     4741 ccgaaaatgg atctctatgc tgaggcttct cacggtaggt atataaagag cctactgccc
     4801 ttcttcaata tgccaaataa atacaaacaa ggttctatac tcaacagtaa tttttcttac
     4861 tcatttaatt acgaaccttg ctctgttttc gcaagttgtt ttcaagtttt caagcttcgc
     4921 gcgggatatc tggcgagcga tcaaaggtgt ttccaatagc tttaaccttt tcatttcctg
     4981 tcctgtattt ttaaataata cattttccgt gtctattact tttattttgg aacaatattg
     5041 aagttcaaat tgtacacgac tttggcaatc ttaggaaaat cttggcatat acaattccaa
     5101 tttttgctct atccaaatat caagactacc atcatcactg aatttatact cttttgtttg
     5161 cgcatcgtct ttcggctttc tttttcacct tctttaatat tttgaaattc tatataggtc
     5221 tttgtgtacc atttaatttt tgtatcttac tttcttttat atcatattta ttagttactt
     5281 atacgttgag tttaccgtcg actctagagg atccccgggt taattaaaat ggtgagcaag
     5341 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     5401 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     5461 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     5521 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     5581 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     5641 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     5701 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     5761 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     5821 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     5881 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     5941 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     6001 cgccactcca ccggcggcat ggacgagctg tacaagtaag gcgcgccact tctaaataag
     6061 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
     6121 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt
     6181 tcctgtaggt caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct
     6241 ctaccggcag atccgctagg gataacaggg taatatagat ctgtttagct tgcctcgtcc
     6301 ccgccgggtc acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac
     6361 gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat
     6421 gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc
     6481 tcctcgctgc agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc
     6541 cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt
     6601 catatacttc ctttttaaat cttgctagga tacagttctc acatcacatc cgaacataaa
     6661 caaccatggg taccactctt gacgacacgg cttaccggta ccgcaccagt gtcccggggg
     6721 acgccgaggc catcgaggca ctggatgggt ccttcaccac cgacaccgtc ttccgcgtca
     6781 ccgccaccgg ggacggcttc accctgcggg aggtgccggt ggacccgccc ctgaccaagg
     6841 tgttccccga cgacgaatcg gacgacgaat cggacgacgg ggaggacggc gacccggact
     6901 ctcggacgtt cgtcgcgtac ggggacgacg gcgacctggc gggcttcgtg gtcgtctcgt
     6961 actccggctg gaaccgccgg ctgaccgtcg aggacatcga ggtcgccccg gagcaccggg
     7021 ggcacggggt cgggcgcgcg ttgatggggc tcgcgacgga gttcgcccgc gagcggggtg
     7081 ccgggcacct ctggctggag gtcaccaacg tcaacgcacc ggcgatccac gcgtaccggc
     7141 ggatggggtt caccctctgc ggcctggaca ccgccctgta cgacggcacc gcctcggacg
     7201 gcgagcaggc gctctacatg agcatgccct gcccctaatc agtactgaca ataaaaagat
     7261 tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta
     7321 atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa
     7381 gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact
     7441 gctgtcgatt cgatactaac gccgccatcc agtttaaacg agctccagct tttgttccct
     7501 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7561 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7621 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7681 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7741 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7801 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7861 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7921 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7981 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8041 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8101 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8161 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8221 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8281 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8341 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8401 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8461 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8521 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8581 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8641 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8701 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8761 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8821 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8881 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8941 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9001 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9061 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9121 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9181 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9241 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9301 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9361 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9421 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9481 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9541 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9601 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9661 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     9721 aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
