LOCUS       sequence-263806-       10194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  4-hydroxytamoxifen inducible vector expressing Flag-NPM1wildtype 
            (human) in mammalian cells through transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Wang AJ, Han Y, Jia N, Chen P, Minden MD
  TITLE     NPM1c impedes CTCF functions through cytoplasmic mislocalization in 
            acute myeloid leukemia.
  JOURNAL   Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. 
            Epub 2019 Dec 12.
  PUBMED    31831844
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-019-0681-8"; journalName: "Leukemia"; date: 
            "2020-05"; volume: "34"; issue: "5"; pages: "1278-1290"
COMMENT     Sequence Label: p5xUAS-Flag-NPM1wt
FEATURES             Location/Qualifiers
     source          1..10194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    501..622
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(538..557)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     592..611
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(671..687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(671..687)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(684..706)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    695..711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(719..725,726..743,744..749))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    764..785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(902..919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1073..1661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1153..1172)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1832..2623,2624..2692))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2455..2474
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2693..2797)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2872..2891)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3063..3442
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3444..3642
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3597..3617
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3660..3840
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3887..4012
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4505..4738
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4923..4967
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5265..5382
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    5447..5541
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5466..5560
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     5606..5622
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      5666..5675
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5675..5698
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        6647..6976
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6827..6962
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6986..7585
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6986..7005)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7482..7502
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7711..8299
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7764..7784)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8182..8193)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8302..8318)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8303..8319)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8824..9004
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9030..9047)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9036..9260
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9306..9734
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9393..9412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9603..9624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9748..10077
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9928..10063
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        10125..10172
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(10191..10194,1..371)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
       61 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
      121 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
      181 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
      241 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
      301 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
      361 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
      421 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
      481 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
      541 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
      601 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
      661 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      721 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      781 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      841 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1261 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1321 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1381 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1441 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1501 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1561 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1621 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1681 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1741 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1801 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1861 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1921 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1981 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2041 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2101 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2161 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2221 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2281 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2341 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2401 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2461 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2521 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2581 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2641 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2701 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2761 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2821 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     2881 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     2941 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     3001 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     3061 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     3121 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     3181 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     3241 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     3301 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     3361 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     3421 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     3481 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     3541 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     3601 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
     3661 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3721 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3781 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3841 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     3901 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     3961 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     4021 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     4081 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     4141 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     4201 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     4261 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     4321 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     4381 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     4441 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     4501 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     4561 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     4621 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     4681 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     4741 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     4801 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     4861 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     4921 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     4981 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     5041 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     5101 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     5161 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     5221 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     5281 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     5341 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     5401 agcagagatc cagtttggtt aattaatagc ttgcatgctt gcaggtcgga gtactgtcct
     5461 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     5521 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagactct agcgggtata
     5581 taatgagatc cagttatcca tcgcagcttg gatacacgcc gcccacgtga aggctgccga
     5641 ccccgggggt ggaccatccg gatccgccac catggattac aaggatgacg acgataagac
     5701 cggtatggaa gattcgatgg acatggacat gagccccctg aggccccaga actatctttt
     5761 cggttgtgaa ctaaaggccg acaaagatta tcactttaag gtggataatg atgaaaatga
     5821 gcaccagtta tctttaagaa cggtcagttt aggggctggt gcaaaggatg agttgcacat
     5881 tgttgaagca gaggcaatga attacgaagg cagtccaatt aaagtaacac tggcaacttt
     5941 gaaaatgtct gtacagccaa cggtttccct tgggggcttt gaaataacac caccagtggt
     6001 cttaaggttg aagtgtggtt cagggccagt gcatattagt ggacagcact tagtagctgt
     6061 ggaggaagat gcagagtcag aagatgaaga ggaggaggat gtgaaactct taagtatatc
     6121 tggaaagcgg tctgcccctg gaggtggtag caaggttcca cagaaaaaag taaaacttgc
     6181 tgctgatgaa gatgatgacg atgatgatga agaggatgat gatgaagatg atgatgatga
     6241 tgattttgat gatgaggaag ctgaagaaaa agcgccagtg aagaaatcta tacgagatac
     6301 tccagccaaa aatgcacaaa agtcaaatca gaatggaaaa gactcaaaac catcatcaac
     6361 accaagatca aaaggacaag aatccttcaa gaaacaggaa aaaactccta aaacaccaaa
     6421 aggacctagt tctgtagaag acattaaagc aaaaatgcaa gcaagtatag aaaaaggtgg
     6481 ttctcttccc aaagtggaag ccaaattcat caattatgtg aagaattgct tccggatgac
     6541 tgaccaagag gctattcaag atctctggca gtggaggaag tctctttaag aaaatagacc
     6601 ggttagtcta gagatccgcg gccgcatcga ttcgaccctg tggaatgtgt gtcagttagg
     6661 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6721 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6781 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6841 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6901 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6961 cctaggcttt tgcaaaaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     7021 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     7081 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     7141 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     7201 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     7261 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     7321 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     7381 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     7441 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     7501 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     7561 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
     7621 gagcgcacga ccccatgcat cgattagtcc aatttgttaa agacaggata acctcgaggg
     7681 cgcgccgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     7741 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7801 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7861 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7921 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7981 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8041 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8101 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8161 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8221 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8281 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
     8341 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     8401 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     8461 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     8521 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     8581 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     8641 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     8701 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     8761 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     8821 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8881 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8941 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     9001 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     9061 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     9121 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     9181 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     9241 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     9301 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9361 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9421 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     9481 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9541 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9601 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     9661 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9721 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     9781 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     9841 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9901 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9961 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
    10021 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    10081 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
    10141 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atgg
//
