LOCUS       sequence-263820-       10179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  4-hydroxytamoxifen inducible vector expressing Flag-NPM1c 
            (cytoplasmic mutant, human) in mammalian cells through transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Wang AJ, Han Y, Jia N, Chen P, Minden MD
  TITLE     NPM1c impedes CTCF functions through cytoplasmic mislocalization in 
            acute myeloid leukemia.
  JOURNAL   Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. 
            Epub 2019 Dec 12.
  PUBMED    31831844
REFERENCE   2  (bases 1 to 10179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-019-0681-8"; journalName: "Leukemia"; date: 
            "2020-05"; volume: "34"; issue: "5"; pages: "1278-1290"
COMMENT     Sequence Label: p5xUAS-Flag-NPM1c
FEATURES             Location/Qualifiers
     source          1..10179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    501..622
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(538..557)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     592..611
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(671..687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(671..687)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(684..706)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    695..711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(719..725,726..743,744..749))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    764..785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(902..919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1073..1661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1153..1172)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1832..2623,2624..2692))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2455..2474
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2693..2797)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2872..2891)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3063..3442
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3444..3642
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3597..3617
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3660..3840
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3887..4012
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4505..4738
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4923..4967
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5265..5382
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    5447..5541
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     5587..5603
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      5647..5656
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5656..5679
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        6632..6961
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6812..6947
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6971..7570
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6971..6990)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7467..7487
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7696..8284
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7749..7769)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8167..8178)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8287..8303)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8288..8304)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8809..8989
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9015..9032)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9021..9245
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9291..9719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9378..9397)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9588..9609
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9733..10062
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9913..10048
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        10110..10157
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(10176..10179,1..371)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
       61 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
      121 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
      181 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
      241 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
      301 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
      361 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
      421 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
      481 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
      541 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
      601 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
      661 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      721 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      781 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      841 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1261 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1321 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1381 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1441 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1501 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1561 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1621 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1681 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1741 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1801 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1861 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1921 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1981 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2041 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2101 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2161 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2221 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2281 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2341 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2401 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2461 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2521 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2581 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2641 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2701 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2761 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2821 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     2881 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     2941 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     3001 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     3061 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     3121 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     3181 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     3241 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     3301 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     3361 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     3421 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     3481 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     3541 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     3601 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
     3661 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3721 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3781 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3841 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     3901 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     3961 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     4021 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     4081 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     4141 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     4201 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     4261 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     4321 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     4381 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     4441 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     4501 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     4561 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     4621 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     4681 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     4741 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     4801 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     4861 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     4921 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     4981 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     5041 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     5101 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     5161 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     5221 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     5281 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     5341 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     5401 agcagagatc cagtttggtt aattaatagc ttgcatgctt gcaggtcgga gtactgtcct
     5461 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     5521 agcggagtac tgtcctccga gcggagactc tagcgggtat ataatgagat ccagttatcc
     5581 atcgcagctt ggatacacgc cgcccacgtg aaggctgccg accccggggg tggaccatcc
     5641 ggatccgcca ccatggatta caaggatgac gacgataaga ccggtatgga agattcgatg
     5701 gacatggaca tgagccccct gaggccccag aactatcttt tcggttgtga actaaaggcc
     5761 gacaaagatt atcactttaa ggtggataat gatgaaaatg agcaccagtt atctttaaga
     5821 acggtcagtt taggggctgg tgcaaaggat gagttgcaca ttgttgaagc agaggcaatg
     5881 aattacgaag gcagtccaat taaagtaaca ctggcaactt tgaaaatgtc tgtacagcca
     5941 acggtttccc ttgggggctt tgaaataaca ccaccagtgg tcttaaggtt gaagtgtggt
     6001 tcagggccag tgcatattag tggacagcac ttagtagctg tggaggaaga tgcagagtca
     6061 gaagatgaag aggaggagga tgtgaaactc ttaagtatat ctggaaagcg gtctgcccct
     6121 ggaggtggta gcaaggttcc acagaaaaaa gtaaaacttg ctgctgatga agatgatgac
     6181 gatgatgatg aagaggatga tgatgaagat gatgatgatg atgattttga tgatgaggaa
     6241 gctgaagaaa aagcgccagt gaagaaatct atacgagata ctccagccaa aaatgcacaa
     6301 aagtcaaatc agaatggaaa agactcaaaa ccatcatcaa caccaagatc aaaaggacaa
     6361 gaatccttca agaaacagga aaaaactcct aaaacaccaa aaggacctag ttctgtagag
     6421 gacattaaag caaaaatgca agcaagtata gaaaaaggtg gttctcttcc caaagtggaa
     6481 gccaaattca tcaattatgt gaagaattgc ttccggatga ctgaccaaga ggctattcaa
     6541 gatctctgtc tggcagtgga ggaagtctct ttaagaaaat agaccggtta gtctagagat
     6601 ccgcggccgc atcgattcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6661 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6721 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6781 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6841 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6901 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6961 aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     7021 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     7081 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     7141 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     7201 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     7261 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     7321 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     7381 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     7441 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     7501 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     7561 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     7621 tgcatcgatt agtccaattt gttaaagaca ggataacctc gagggcgcgc cgaattcgat
     7681 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7741 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7801 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7861 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7921 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     7981 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8041 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     8101 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8161 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8221 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8281 ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     8341 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     8401 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     8461 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     8521 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     8581 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     8641 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     8701 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     8761 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     8821 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8881 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8941 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
     9001 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     9061 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     9121 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     9181 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     9241 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     9301 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     9361 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     9421 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     9481 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     9541 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     9601 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     9661 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     9721 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     9781 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     9841 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     9901 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9961 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
    10021 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
    10081 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
    10141 gcatagtata atacgacaag gtgaggaact aaaccatgg
//
