LOCUS       sequence-263857-        5907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC38A10
FEATURES             Location/Qualifiers
     source          1..5907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(807..824)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     protein_bind    complement(4032..4131)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4148..4167)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4149..4167)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4172..4188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4172..4188)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4290..4309)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4301..5110
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4368..4387)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5257..5845
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5746..5765
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgaccg cagcagcagc aagcaactgg ggactgatca caaacatcgt
      721 gaattctatc gtgggcgtga gcgtgctgac catgcccttc tgctttaagc agtgtggaat
      781 cgtgctgggc gccctgctgc tggtgttttg ctcttggatg acccaccaga gctgtatgtt
      841 cctggtgaag tctgccagcc tgtccaagcg gagaacatac gccggcctgg cctttcatgc
      901 gtatggaaag gcggggaaga tgctggtgga gaccagcatg atcggcctga tgctgggcac
      961 atgcatcgcc ttctatgtgg tcatcggcga cctgggctcc aacttctttg ccagactgtt
     1021 cggctttcaa gtgggcggca ccttcaggat gtttctgctg ttcgccgtga gcctgtgcat
     1081 cgtgctgcct ctgtccctgc agagaaatat gatggcctct atccagtctt ttagcgccat
     1141 ggccctgctg ttctacacag tgttcatgtt tgtgatcgtg ctgagctccc tgaagcacgg
     1201 cctgtttagc ggccagtggc tgaggcgcgt gtcctatgtg cgctgggagg gcgtgttccg
     1261 gtgcatccca atctttggca tgtccttcgc ctgtcagtct caggtgctgc ccacctacga
     1321 cagcctggat gagccttccg tgaagacaat gtctagcatc tttgcctcct ctctgaatgt
     1381 ggtgaccaca ttctacgtga tggtgggctt ctttggctat gtgagcttta ccgaggccac
     1441 agccggcaac gtgctgatgc acttcccctc caatctggtg accgagatgc tgcgggtggg
     1501 ctttatgatg tctgtggccg tgggcttccc catgatgatc ctgccttgca gacaggccct
     1561 gtccaccctg ctgtgcgagc agcagcagaa ggacggcaca tttgccgcag gaggatatat
     1621 gccacctctg cggtttaagg ccctgaccct gagcgtggtg ttcggcacaa tggtgggcgg
     1681 catcctgatc ccaaacgtgg agaccatcct gggactgacc ggagcaacaa tgggatccct
     1741 gatctgcttc atctgtcccg ccctgatcta caagaagatc cacaagaatg ccctgagctc
     1801 ccaggtggtg ctgtgggtgg gactgggcgt gctggtggtg agcacagtga ccacactgtc
     1861 cgtgtctgag gaagtgccag aggatctggc agaggaggca ccaggaggca gactgggcga
     1921 ggcagaggga ctgatgaagg tggaggcagc caggctgtcc gcccaggacc cagtggtggc
     1981 agtggcagag gatggccgcg agaagccaaa gctgccaaag gagagggagg agctggagca
     2041 ggcccagatc aagggacctg tggacgtgcc aggaagggag gatggcaagg aggcacctga
     2101 ggaggcacag ctggacaggc caggccaggg catcgccgtg cccgtgggcg aggcccacag
     2161 acacgagcca cccgtgcctc acgataaggt ggtggtggac gagggacagg atagggaggt
     2221 gccagaggag aacaagcctc catctaggca cgcaggaggc aaggcaccag gagtgcaggg
     2281 ccagatggcc ccccctctgc cagacagcga gagggagaag caggagcctg agcagggcga
     2341 agtgggcaag cggccaggac aggcacaggc cctggaggag gcaggcgacc tgcctgagga
     2401 tccacagaag gtgcctgagg cagatggaca gccagcagtg cagcctgcaa aggaggacct
     2461 gggaccaggc gatagaggac tgcacccaag gcctcaggcc gtgctgtccg agcagcagaa
     2521 cggactggca gtgggaggag gagagaaggc caagggagga ccaccacctg gaaatgcagc
     2581 aggcgacacc ggccagccag ccgaggactc tgatcacggc ggcaagccac cactgccagc
     2641 agagaagcca gcaccaggac ctggcctgcc tccagagcca agggagcaga gggatgtgga
     2701 gagggcagga ggaaatcagg cagcaagcca gctggaggag gcaggacgcg ccgagatgct
     2761 ggaccacgcc gtgctgctgc aggtcatcaa ggagcagcag gtgcagcaga agcggctgct
     2821 ggatcagcag gagaagctgc tggccgtgat cgaggagcag cacaaggaga tccaccagca
     2881 gaggcaggag gacgaggagg ataagccaag gcaggtggag gtgcaccagg agccaggagc
     2941 agcagtgcca aggggacagg aggcacctga gggcaaggca agggagaccg tggagaacct
     3001 gccacctctg ccactggacc ctgtgctgag ggcaccagga ggccggccag caccctccca
     3061 ggatctgaat cagagaagcc tggagcactc cgagggccct gtgggcaggg acccagcagg
     3121 accacctgac ggaggaccag ataccgagcc cagagcagca caggcaaagc tgagggacgg
     3181 acagaaggat gcagcaccaa gggcagccgg cacagtgaag gagctgccaa agggaccaga
     3241 gcaggtgcct gtgccagatc cagcaagaga ggcaggagga ccagaggaga ggctggcaga
     3301 ggagttccct ggacagtccc aggacgtgac cggaggctct caggatcgca agaagcctgg
     3361 caaggaggtg gcagcaaccg gcacatccat cctgaaggag gcaaactggc tggtggcagg
     3421 accaggagca gagacaggcg accctcgcat gaagccaaag caggtgtctc gggacctggg
     3481 cctggccgcc gatctgccag gaggagcaga gggagcagca gcacagcctc aggccgtgct
     3541 gagacagcca gagctgagag tgatctctga cggagagcag ggaggacagc agggacacag
     3601 gctggatcac ggcggccacc tggagatgag aaaggcaagg ggaggcgacc acgtgcccgt
     3661 gagccacgag cagcctaggg gaggcgagga tgccgccgtg caggagccta gacagaggcc
     3721 tgagccagag ctgggcctga agagggcagt gccaggagga cagaggcctg acaacgccaa
     3781 gccaaatcgg gatctgaagc tgcaggcagg aagcgacctg cggagaaggc gccgggatct
     3841 gggacctcac gcagagggac agctggcacc aagagacggc gtgatcatcg gcctgaaccc
     3901 cctgcctgac gtgcaagtga atgatctgag aggcgccctg gatgcacagc tgaggcaggc
     3961 agcaggaggc gccctgcagg tggtgcactc tcgccagctg cggcaggccc ccggccctcc
     4021 agaggagagc gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     4081 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     4141 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     4201 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     4261 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     4321 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     4381 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     4441 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     4501 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     4561 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     4621 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     4681 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     4741 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     4801 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     4861 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     4921 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     4981 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     5041 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     5101 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     5161 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     5221 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5281 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5341 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5401 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5461 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5521 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5581 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5641 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5701 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5761 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5821 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5881 cttttgctgg ccttttgctc acatgtt
//
