LOCUS       sequence-263865-        4467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC34A1
FEATURES             Location/Qualifiers
     source          1..4467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2592..2691)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2708..2727)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2709..2727)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2732..2748)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2732..2748)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2850..2869)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2861..3670
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2928..2947)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3817..4405
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4306..4325
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgctga gctacggaga gaggctggga tccccagccg tgtctcccct
      721 gcctgtgcgg ggaggacacg tgatgagagg aaccgccttt gcctacgtgc catccccaca
      781 ggtgctgcac aggatcccag gcacaagcgc ctatgcattc ccatccctgg gaccagtggc
      841 cctggcagag cacacctgcc cttgtggaga ggtgctggag aggcacgagc ctctgccagc
      901 aaagctggcc ctggaggagg agcagaagcc cgagagcagg ctggtgccta agctgaggca
      961 ggcaggagca atgctgctga aggtgcctct gatgctgacc tttctgtatc tgttcgtgtg
     1021 ctccctggac atgctgagct ccgcctttca gctggcagga ggcaaggtgg caggcgacat
     1081 cttcaaggat aacgccatcc tgtctaatcc tgtggcagga ctggtggtgg gaatcctggt
     1141 gacagtgctg gtgcagtcta gctccacctc cacatctatc atcgtgtcca tggtgtctag
     1201 cggcctgctg gaggtgtcct ctgccatccc aatcatcatg ggctctaaca tcggcaccag
     1261 cgtgaccaat acaatcgtgg ccctgatgca ggcaggcgac aggaccgatt ttcggagagc
     1321 cttcgccgga gcaacagtgc acgattgttt caattggctg tctgtgctgg tgctgctgcc
     1381 actggaggca gcaaccggat acctgcacca catcacaagg ctggtggtgg ccagctttaa
     1441 catccacgga ggaagggacg caccagatct gctgaagatc atcaccgagc ctttcacaaa
     1501 gctgatcatc cagctggacg agagcgtgat cacctccatc gccacaggcg atgagtccct
     1561 gaggaatcac tctctgatcc agatctggtg ccacccagac agcctgcagg cacccaccag
     1621 catgtccagg gcagaggcaa acagctccca gaccctgggc aatgccacaa tggagaagtg
     1681 taaccacatc tttgtggaca caggcctgcc tgatctggca gtgggactga tcctgctggc
     1741 aggatctctg gtgctgctgt gcacctgtct gatcctgctg gtgaagatgc tgaatagcct
     1801 gctgaagggc caggtggcca aagtgatcca gaaagtgatc aacacagatt ttcccgcccc
     1861 tttcacctgg gtgacaggct acttcgccat ggtggtgggc gccagcatga cctttgtggt
     1921 gcagtctagc tccgtgttca cctccgccat cacaccactg atcggactgg gcgtgatctc
     1981 catcgagagg gcatatccac tgaccctggg atctaatatc ggcaccacaa ccacagcaat
     2041 cctggccgcc ctggcaagcc caagagagaa gctgtctagc gcctttcaga tcgccctgtg
     2101 ccacttcttt ttcaacatct ccggcatcct gctgtggtat ccagtgccct gtaccaggct
     2161 gccaatcagg atggcaaagg ccctgggcaa gaggacagcc aagtaccgct ggttcgccgt
     2221 gctgtatctg ctggtgtgct ttctgctgct gccttctctg gtgttcggca tcagcatggc
     2281 aggatggcag gtcatggtgg gagtgggcac accatttggc gccctgctgg ccttcgtggt
     2341 gctgatcaat gtgctgcagt ctcggagccc tggacacctg ccaaagtggc tgcagacctg
     2401 ggactttctg cctcggtgga tgcactccct gaagccactg gatcacctga tcaccagagc
     2461 cacactgtgc tgtgcaaggc ctgagccaag atccccacct ctgccaccca gagtgttcct
     2521 ggaggagctg cctccagcaa ccccatctcc taggctggcc ctgccagcac accacaacgc
     2581 aacaagactg gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     2641 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2701 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2761 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     2821 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     2881 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2941 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     3001 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     3061 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     3121 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     3181 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     3241 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     3301 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     3361 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     3421 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     3481 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     3541 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     3601 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     3661 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     3721 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     3781 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3841 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3901 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3961 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4021 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4081 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4141 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4201 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4261 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4321 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4381 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4441 cttttgctgg ccttttgctc acatgtt
//
