LOCUS       sequence-263866-        3798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC35D3
FEATURES             Location/Qualifiers
     source          1..3798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1923..2022)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2039..2058)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2040..2058)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2063..2079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2063..2079)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2181..2200)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2192..3001
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2259..2278)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3148..3736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3637..3656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgaggc agctgtgcag gggaagagtg ctgggaatct ccgtggcaat
      721 cgcacacggc gtgttcagcg gatccctgaa catcctgctg aagtttctga tcagcagata
      781 ccagttctcc tttctgaccc tggtgcagtg cctgaccagc tccacagccg ccctgtccct
      841 ggagctgctg cggagactgg gactgatcgc agtgccacct ttcggcctgt ccctggcaag
      901 gtcttttgcc ggagtggccg tgctgtctac cctgcagtct agcctgacac tgtggtctct
      961 gaggggactg agcctgccaa tgtatgtggt gttcaagaga tgtctgcctc tggtgacaat
     1021 gctgatcggc gtgctggtgc tgaagaatgg agcaccaagc ccaggcgtgc tggcagccgt
     1081 gctgatcacc acatgcggag ccgccctggc aggagcaggc gacctgaccg gcgatcccat
     1141 cggctacgtg acaggcgtgc tggccgtgct ggtgcacgca gcatatctgg tgctgatcca
     1201 gaaggcaagc gccgacaccg agcacggacc actgacagcc cagtacgtga tcgccgtgtc
     1261 cgccacccca ctgctggtca tctgttcttt cgccagcacc gactccatcc acgcctggac
     1321 atttccaggc tggaaggatc ccgccatggt gtgcatcttc gtggcctgta tcctgatcgg
     1381 ctgcgccatg aactttacca cactgcactg cacatatatc aattccgccg tgaccacatc
     1441 ttttgtgggc gtggtgaagt ccatcgccac catcacagtg ggcatggtgg ccttcagcga
     1501 tgtggagcct acctccctgt ttatcgccgg cgtggtggtg aacacactgg gctccatcat
     1561 ctactgcgtg gccaagttca tggagaccag gaagcagtct aattatgagg acctggaggc
     1621 acagcctagg ggagaggagg cccagctgtc tggcgatcag ctgccttttg tgatggagga
     1681 gctgccagga gagggaggaa acggcagaag cgagggagga gaggcagcag gaggaccagc
     1741 ccaggagtct agacaggagg tgaggggaag cccaagggga gtgcctctgg tggcaggatc
     1801 ctctgaggag ggatctaggc gcagcctgaa ggacgcctac ctggaagtgt ggaggctggt
     1861 gcgcggaacc cggtacatga agaaggatta tctgatcgag aatgaggagc tgcctagccc
     1921 agacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     1981 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     2041 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2101 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     2161 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     2221 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     2281 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     2341 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2401 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2461 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     2521 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     2581 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     2641 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     2701 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     2761 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     2821 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     2881 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     2941 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     3001 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     3061 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3781 gccttttgct cacatgtt
//
