LOCUS       sequence-263870-        6502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses YFP-NOP56 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Newhart A, Powers SL, Shastrula PK, Sierra I, Joo LM, Hayden JE, 
            Cohen AR, Janicki SM
  TITLE     RNase P protein subunit Rpp29 represses histone H3.3 nucleosome 
            deposition.
  JOURNAL   Mol Biol Cell. 2016 Apr 1;27(7):1154-69. doi: 
            10.1091/mbc.E15-02-0099. Epub 2016 Feb 3.
  PUBMED    26842893
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E15-02-0099"; journalName: "Mol Biol Cell"; date: 
            "2016-04-1- 1"; volume: "27"; issue: "7"; pages: "1154-69"
COMMENT     Sequence Label: YFP-NOP56-C3
FEATURES             Location/Qualifiers
     source          1..6502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      243..252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(249..251,252..254,255..965)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(294..315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(555..574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     902..923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2926..3047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2963..2982)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3017..3036
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3054..3509)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3191..3212)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3403..3422
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3536..3640
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3642..3999
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3642..3833
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3665..3685)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3850..3985
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4034..4828
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4034..4828
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4088..4107)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4698..4717
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5016..5035)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5060..5107
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5436..6024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5925..5944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6199..6502
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      301 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      361 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      421 ggcccaccct cgtgaccacc ttcggctacg gcctgaagtg cttcgcccgc taccccgacc
      481 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      541 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      601 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      661 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      721 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      781 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      841 acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
      901 acatggtcct gctggagttc gtgaccgccg ccgggatcac tcacggcatg gacgagctgt
      961 acaagtactc agatctcgag atggtgctgt tgcacgtgct gtttgagcac gcggtcggct
     1021 acgcgctgct ggcgctgaag gaagtggagg agatcagtct gctgcagccg caggtggagg
     1081 agtccgtgct caacctgggc aaattccaca gcatcgttcg tctggtggcc ttttgtccct
     1141 ttgcctcatc ccaggttgcc ttggaaaatg ccaacgccgt gtctgaaggg gttgttcatg
     1201 aggacctccg cctgctcttg gagacccacc tgccgtccaa aaagaagaaa gtactcttgg
     1261 gagttgggga tcccaagatt ggtgccgcaa tacaggagga gttagggtac aactgccaga
     1321 ctggaggagt catagctgag atcctgcgag gagttcgtct gcacttccac aatctggtga
     1381 agggtctgac cgatctgtca gcttgtaaag cacagctggg gctgggacac agctattccc
     1441 gtgccaaagt taagtttaat gtgaaccggg tggacaatat gatcatccag tccattagcc
     1501 tcctggacca gctggataag gacatcaata ccttctctat gcgtgtcagg gagtggtacg
     1561 ggtatcactt tccggagctg gtgaagatca tcaacgacaa tgccacatac tgccgtcttg
     1621 cccagtttat tggaaaccga agggaactga atgaggacaa gctggagaag ctggaggagc
     1681 tgacaatgga tggggccaag gctaaggcta ttctggatgc ctcacggtcc tccatgggca
     1741 tggacatatc tgccattgac ttgataaaca tcgagagctt ctccagtcgt gtggtgtctt
     1801 tatctgaata ccgccagagc ctacacactt acctgcgctc caagatgagc caagtagccc
     1861 ccagcctgtc agccctaatt ggggaagcgg taggtgcacg tctcatcgca catgctggca
     1921 gcctcaccaa cctggccaag tatccagcat ccacagtgca gatccttggg gctgaaaagg
     1981 ccctgttcag agccctgaag acaaggggta acaccccaaa atatggactc attttccact
     2041 ccaccttcat tggccgagca gctgccaaga acaaaggccg catctcccga tacctggcaa
     2101 acaaatgcag tattgcctca cgaatcgatt gcttctctga ggtgcccacg agtgtattcg
     2161 gggagaagct tcgagaacaa gttgaagagc gactgtcctt ctatgagact ggagagatac
     2221 cacgaaagaa tctggatgtc atgaaggaag caatggttca ggcagaggaa gcggctgctg
     2281 agattactag gaagctggag aaacaggaga agaaacgctt aaagaaggaa aagaaacggc
     2341 tggctgcact tgccctcgcg tcttcagaaa acagcagtag tactccagag gagtgtgagg
     2401 agatgagtga aaaacccaaa aagaagaaaa agcaaaagcc ccaggaggtt cctcaggaga
     2461 atggaatgga agacccatct atctctttct ccaaacccaa gaaaaagaaa tctttttcca
     2521 aggaggagtt gatgagtagc gatcttgaag agaccgctgg cagcaccagt attcccaaga
     2581 ggaagaagtc tacacccaag gaggaaacag ttaatgaccc tgaggaggca ggccacagaa
     2641 gtggctccaa gaaaaagagg aaattctcca aagaggagcc ggtcagcagt gggcctgaag
     2701 aggcggttgg caagagcagc tccaagaaga agaaaaagtt ccataaagca tcccaggaag
     2761 actaagaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg
     2821 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     2881 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     2941 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3001 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt
     3061 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     3121 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     3181 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3241 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3301 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     3361 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3421 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3481 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     3541 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3601 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     3661 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3721 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3781 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3841 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3901 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3961 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     4021 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     4081 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4141 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4201 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4261 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4321 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4381 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4441 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4501 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4561 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4621 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4681 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4741 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4801 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     4861 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4921 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     4981 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     5041 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     5101 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     5161 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     5221 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     5281 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5341 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5401 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5461 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5521 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5581 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5641 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5701 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5761 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5821 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5881 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5941 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6001 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6061 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6121 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
     6181 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     6241 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     6301 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     6361 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     6421 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     6481 attagtcatc gctattacca tg
//
