LOCUS       sequence-26389-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag PDK1
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..81
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    136..160
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccttctcta ggcgccggcc ggatccagaa ttcgccacca tggggtcttc aaaatctaaa
       61 ccaaaggacc ccagccagcg ccggcgcagg atccgaggtt accttgacta caaagacgat
      121 gacgacaagc aattgacaag tttgtacaaa aaagttggca tgaggctggc gcggctgctt
      181 cgcggagccg ccttggccgg cccgggcccg gggctgcgcg ccgccggctt cagccgcagc
      241 ttcagctcgg actcgggctc cagcccggcg tccgagcgcg gcgttccggg ccaggtggac
      301 ttctacgcgc gcttctcgcc gtccccgctc tccatgaagc agttcctgga cttcggatca
      361 gtgaatgctt gtgaaaagac ctcatttatg tttctgcggc aagagttgcc tgtcagactg
      421 gcaaatataa tgaaagaaat aagtctcctt ccagataatc ttctcaggac accatccgtt
      481 caattggtac aaagctggta tatccagagt cttcaggagc ttcttgattt taaggacaaa
      541 agtgctgagg atgctaaagc tatttatgac tttacagata ctgtgatacg gatcagaaac
      601 cgacacaatg atgtcattcc cacaatggcc canggtgtga ttgaatacaa ngagagcttt
      661 ggggtggatc ctgtcaccag ccanaatgtt cagtactttt tggatcgatt ctacatgagt
      721 cgcatttc
//
