LOCUS       sequence-263898-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tagging genes in Cterm with mScarletI in fission yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  TITLE     pFA6a-mScarletI-KanMX6
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-mScarletI-KanMX6
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..693
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(131..149)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     399..418
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgagcaagg gcgaggcagt gatcaaggag ttcatgcggt tcaaggtgca catggagggc
       61 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      121 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc ctgggacatc
      181 ctgtcccctc agttcatgta cggctccagg gccttcatca agcaccccgc cgacatcccc
      241 gactactata agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      301 gacggcggcg ccgtgaccgt gacccaggac acctccctgg aggacggcac cctgatctac
      361 aaggtgaagc tccgcggcac caacttccct cctgacggcc ccgtaatgca gaagaagaca
      421 atgggctggg aagcgtccac cgagcggttg taccccgagg acggcgtgct gaagggcgac
      481 attaagatgg ccctgcgcct gaaggacggc ggccgctacc tggcggactt caagaccacc
      541 tacaaggcca agaagcccgt gcagatgccc ggcgcctaca acgtcgaccg caagttggac
      601 atcacctccc acaacgagga ctacaccgtg gtggaacagt acgaacgctc cgagggccgc
      661 cactccaccg gcggcatgga cgagctgtac aagtag
//
