LOCUS       sequence-263899-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tagging genes in Cterm with mScarletI in fission yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     pFA6a-mScarletI-KanMX6
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-mScarletI-KanMX6
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..810
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(68..87)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 atgggtaagg aaaagactca cgtttcgagg ccgcgattaa attccaacat ggatgctgat
       61 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
      121 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
      181 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
      241 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
      301 ggcaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
      361 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
      421 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
      481 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
      541 cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
      601 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
      661 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
      721 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
      781 tttcatttga tgctcgatga gtttttctaa
//
