LOCUS       sequence-263905-        5064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_OCA2
FEATURES             Location/Qualifiers
     source          1..5064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3189..3288)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3305..3324)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3306..3324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3329..3345)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3329..3345)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3447..3466)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3458..4267
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3525..3544)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4414..5002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4903..4922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgcacc tggagggaag ggacggccgg agataccctg gagcaccagc
      721 agtggagctg ctgcagacca gcgtgccttc cggactggca gagctggtgg caggcaagag
      781 gcgcctgcca aggggagcag gaggagcaga tcctagccac tcctgcccac gcggcgccgc
      841 cggccagagc tcctgggccc cagccggcca ggagttcgcc tcctttctga caaagggccg
      901 gtctcactct agcctgccac agatgtcctc tagcagatct aaggacagct gcttcaccga
      961 gaacacaccc ctgctgcgga atagcctgca ggagaagggc tccagatgta tcccagtgta
     1021 ccaccccgag tttatcaccg ccgaggagag ctgggaggac tcctctgccg attgggagcg
     1081 gagatatctg ctgtctaggg aggtgagcgg actgtctgcc agcgccagct ccgagaaggg
     1141 cgacctgctg gatagccccc acatccggct gagactgtcc aagctgaggc gctgcgtgca
     1201 gtggctgaaa gtgatgggcc tgttcgcctt cgtggtgctg tgctccatcc tgttctctct
     1261 gtaccctgac cagggcaagc tgtggcagct gctggccctg tctccactgg agaactatag
     1321 cgtgaatctg tctagccacg tggactctac actgctgcag gtggatctgg caggcgccct
     1381 ggtggcatcc ggaccatctc ggcctggcag agaggagcac atcgtggtgg agctgaccca
     1441 ggcagatgca ctgggatcca ggtggcggag accccagcag gtgacacaca actggaccgt
     1501 gtacctgaat cctaggcgca gcgagcactc cgtgatgtct aggacatttg aggtgctgac
     1561 ccgcgagaca gtgtccatct ctatcagggc atctctgcag cagacccagg cagtgcctct
     1621 gctgatggca caccagtacc tgagaggcag cgtggagacc caggtgacaa tcgcaaccgc
     1681 aatcctggca ggcgtgtatg ccctgatcat cttcgagatc gtgcacagga cactggccgc
     1741 catgctggga tctctggccg ccctggccgc cctggccgtg atcggcgaca ggccaagcct
     1801 gacacacgtg gtggagtgga tcgatttcga gaccctggcc ctgctgtttg gcatgatgat
     1861 cctggtggcc atctttagcg agaccggctt ctttgactac tgcgccgtga aggcctatag
     1921 gctgtccagg ggccgcgtgt gggcaatgat catcatgctg tgcctgatcg cagccgtgct
     1981 gagcgccttc ctggacaacg tgaccacaat gctgctgttt acaccagtga ccatcaggct
     2041 gtgcgaggtg ctgaacctgg atccccgcca ggtgctgatc gccgaagtga tcttcaccaa
     2101 tatcggagga gcagcaaccg caatcggcga cccccctaac gtgatcatcg tgagcaatca
     2161 ggagctgcgg aagatgggcc tggatttcgc cggctttacc gcccacatgt tcatcggcat
     2221 ctgcctggtg ctgctggtgt gctttcccct gctgcggctg ctgtactgga acagaaagct
     2281 gtataataag gagccttccg agatcgtgga gctgaagcac gagatccacg tgtggaggct
     2341 gacagcccag cgcatcagcc ctgcatccag agaggagacc gccgtgcgga gactgctgct
     2401 gggcaaggtg ctggccctgg agcacctgct ggcaaggcgc ctgcacacat tccaccggca
     2461 gatctcccag gaggacaaga actgggagac caatatccag gagctgcaga agaagcacag
     2521 aatctctgat ggcatcctgc tggccaagtg tctgaccgtg ctgggcttcg tgatctttat
     2581 gttctttctg aatagctttg tgcccggcat ccacctggat ctgggctgga tcgccatcct
     2641 gggcgccatc tggctgctga tcctggccga catccacgat ttcgagatca tcctgcacag
     2701 ggtggagtgg gccacactgc tgttctttgc cgccctgttt gtgctgatgg aggccctggc
     2761 ccacctgcac ctgatcgagt acgtgggcga gcagaccgcc ctgctgatca agatggtgcc
     2821 tgaggagcag cgcctgatcg cagccatcgt gctggtggtg tgggtgagcg ccctggcatc
     2881 ctctctgatc gacaacatcc cattcaccgc cacaatgatc cccgtgctgc tgaatctgtc
     2941 ccacgatcca gaagtgggac tgcctgcacc accactgatg tatgcactgg cctttggcgc
     3001 atgcctggga ggaaacggca cactgatcgg agcatccgcc aacgtggtgt gcgcaggaat
     3061 cgcagagcag cacggctacg gcttctcctt tatggagttc tttagactgg gcttccccat
     3121 gatggtggtg tcttgcaccg tgggcatgtg ctatctgctg gtggcacacg tggtggtggg
     3181 atggaacgac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     3241 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     3301 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     3361 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     3421 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     3481 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     3541 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     3601 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     3661 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     3721 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     3781 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     3841 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     3901 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     3961 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     4021 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     4081 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     4141 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     4201 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     4261 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     4321 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     4381 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4441 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4501 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4561 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4621 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4861 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4921 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4981 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5041 ttgctggcct tttgctcaca tgtt
//
