LOCUS       sequence-263906-        6777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses YFP-Sp100B fuson protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Newhart A, Negorev DG, Rafalska-Metcalf IU, Yang T, Maul GG, Janicki
            SM
  TITLE     Sp100A promotes chromatin decondensation at a CMV-promoter regulated
            transcription site.
  JOURNAL   Mol Biol Cell. 2013 Mar 13.
  PUBMED    23485562
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2013 Mar 13."
COMMENT     Sequence Label: YFP-Sp100B-C3
FEATURES             Location/Qualifiers
     source          1..6777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        33..224
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     181..201
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(226..330)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      357..812
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(444..463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     654..675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    819..940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(830..849)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     884..903
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(3176..3886,3887..3889,3890..3892))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3218..3239)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3567..3586
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3826..3847
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3889..3898
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3937..4140)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3966..3986)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4141..4444
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4619..5207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4699..4718)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5536..5583
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5608..5627
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5815..6609)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5815..6609)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5926..5945)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6536..6555
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(6644..6777,1..224))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(6658..6777,1..16)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
       61 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
      121 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
      181 accctaactg acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca
      241 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
      361 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      421 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      481 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      541 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      601 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      661 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
      721 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
      781 gaattttaac aaaatattaa cgcttacaat ttacgcgtta agatacattg atgagtttgg
      841 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      901 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
      961 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     1021 caaatgtggt atggctgatt atgatcagtt atctagatcc ggtggatccc gggcccgcgg
     1081 tacctcactt gatcatcacc ttttttcttg tgcttggtgg ttctggcaga aatttgttct
     1141 ccatcaggac tttcagggta tatccaccgc agcgtatact tagcttccag ttcttggatg
     1201 ctccgcggtc tccttcaatt tcaaattccc tgggagtgaa ccacttttta tcctcactct
     1261 gtatacactt ctttgaggtt ccttgtttga atcgctcctt atatagagtg cccttcacct
     1321 caccacaggt cacaggaagt tcagattgtt taaaattaat attttcatct ttgggaattc
     1381 ttggacctct ttttcttctt cttttcaaag gtctagtgtt ggcttttctt cctctttgtt
     1441 gccatctttt cttttggact ttgttattca gagttaaatg ttttctaggt ctgtctttct
     1501 ttctccctct ttggagacca tttactttag atctatgcct tctctttttt cttcttttcc
     1561 cactgtgttt ttccaaagta gaattgtttt caacatccat ggtatccatc atgtcagatg
     1621 cttgactact ttcagtcctt gcttcttggc ttcttggcac accttttgga aaacacatga
     1681 cacaggagca cttcttattt tcaggttctt gtggctgtga tcctgaccct cttcttaggg
     1741 atgagctgca ggtttcctga agctcttctc cattactcag gtctgaaaag tcactactgc
     1801 tgaaacgtct cttcctgctg ggtattctcc aagtagatgt acttctagaa gtcataggag
     1861 ccttctcacc atgcttgctt ctcagtgccc cgcttgaggc ttctgcgggc gcctcctcct
     1921 cactggattc tgaaaagtcg tggtcttgtc ctatcactct tttcttaaaa ctttctctga
     1981 atgtagataa ttttctgaaa tccatgggct ctggtacaat ctggggtcgt gagcaagtgg
     2041 cttcttggcc ctccttttca tcatttgatt ctaaagggtt gtcattattg atcacaggct
     2101 cacttctcgg tgctgagatg aagacttcta agggctcatc aacgtcagtg gatccttcag
     2161 agtcctcact gctgatgact attatgtcag atgcctggtt atgatgagtt cttgcttggg
     2221 cttggcactc aacttttgaa ttagaaaatg gcttttcctt ttttatatcc accagtcgca
     2281 cagaacagga attaatttgg attccatggt tgtgtagctc tgcctctggg ctttcttcat
     2341 cacagggcaa cgggcagggc atctcccttc cagcatcccc attattcact tggacagcaa
     2401 tttgttcgca ggactctgtt ggctcagcct tttgagcaca ttgttcattt gtttgttggc
     2461 ttcctagcga atcatctttg tcactggttg tatcttttct ctttgcattt atctgttctg
     2521 tttcacaggg gtgctctgag agtccatttt caggtggggt tgtaccagca tgagatgggg
     2581 aacccgaagg tggccaagtc aggcttcgaa aagagttttc accagttcct tgttcaagac
     2641 ttagttggag gccagacctc tcctccctct cttcttcttc actttcttgg agaggcaatt
     2701 tgtcatggat tacattttca aagcctttat aaatgtgaat taaatcgggg tattcctgca
     2761 tgttgacatc gctgaacagt gcttccagaa ctggcaggtt aaatgtcttc tccagttcac
     2821 taagaacatt gtacaccact ctctgtacag ggaccaggtt tctacaagaa tcttgagaat
     2881 cttcaaacat tttatttgtg atgagatcac gatcacggag gccctcgagg aatggaaatg
     2941 tcttttttat tgcatttgaa atctccacct tatttctttt gaagtgcttg aatacaatgt
     3001 catagagcaa cctgtcatct acaccctggt cttccgtgaa catcctttgc aaatcgtggc
     3061 tgtgtgcagg aagatggttc atctcatttg ctactggtga aatacattca ttcagcctcc
     3121 tggtgctcag gtcgccgcct ccacctgcca taagcttgag ctcgagatct gagtacttgt
     3181 acagctcgtc catgccgtga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
     3241 gatcgcgctt ctcgttgggg tctttgctca gcttggactg gtagctcagg tagtggttgt
     3301 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
     3361 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
     3421 gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
     3481 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
     3541 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
     3601 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
     3661 ggtcggggta gcgggcgaag cacttcaggc cgtagccgaa ggtggtcacg agggtgggcc
     3721 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
     3781 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
     3841 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcga
     3901 ccggtagcgc tagcggatct gacggttcac taaaccagct ctgcttatat agacctccca
     3961 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     4021 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     4081 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     4141 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     4201 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     4261 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     4321 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     4381 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     4441 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     4501 actaatgcat ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4561 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4621 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4681 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4741 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4801 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4861 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4921 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4981 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5041 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5101 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5161 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5221 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5281 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5341 caatctaaag tatatatgag taacctgagg ctatggcagg gcctgccgcc ccgacgttgg
     5401 ctgcgagccc tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc
     5461 gggcgtattg gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc
     5521 gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt tttattctgt ctttttattg
     5581 ccgtcatagc gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc
     5641 tagggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt
     5701 cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc
     5761 gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa
     5821 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     5881 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     5941 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     6001 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     6061 atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc
     6121 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     6181 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     6241 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     6301 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     6361 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     6421 ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     6481 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     6541 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
     6601 ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc
     6661 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     6721 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcg
//
