LOCUS       sequence-263912-        4722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLCO4C1
FEATURES             Location/Qualifiers
     source          1..4722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2847..2946)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2963..2982)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2964..2982)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2987..3003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2987..3003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3105..3124)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3116..3925
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3183..3202)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4072..4660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4561..4580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgaaga gcgccaaggg catcgagaac ctggccttcg tgcccagctc
      721 ccctgacatc ctgcggagac tgtccgcctc tccaagccag atcgaggtgt ccgccctgtc
      781 tagcgatcca cagcgggaga attctcagcc ccaggagctg cagaagccac aggagcccca
      841 gaagtctcca gagccatctc tgccaagcgc cccacctaac gtgagcgagg agaagctgag
      901 atccctgtct ctgagcgagt tcgaggaggg cagctatggc tggaggaatt ttcaccctca
      961 gtgcctgcag cggtgcaaca caccaggcgg cttcctgctg cactactgcc tgctggcagt
     1021 gacccaggga atcgtggtga atggcctggt gaacatctcc atctctacag tggagaagcg
     1081 gtatgagatg aagtcctctc tgaccggcct gatcagctcc tcttacgaca tctccttctg
     1141 tctgctgtcc ctgttcgtga gcttcttcgg agagagggga cacaagccaa gatggctggc
     1201 cttcgccgcc tttatgatcg gactgggcgc cctggtgttc tctctgcctc agttctttag
     1261 cggcgagtat aagctgggct ctctgtttga ggatacatgc gtgaccacaa ggaatagcac
     1321 ctcctgtaca agctccacct ctagcctgtc caactatctg tacgtgttca tcctgggaca
     1381 gctgctgctg ggagcaggag gcaccccact gtacacactg ggcaccgcct ttctggacga
     1441 tagcgtgccc acacacaagt cctctctgta tatcggcacc ggctacgcca tgtccatcct
     1501 gggacctgca atcggatatg tgctgggagg ccagctgctg accatctaca tcgacgtggc
     1561 catgggcgag agcacagatg tgaccgagga cgatcctagg tggctgggag catggtggat
     1621 cggcttcctg ctgtcctgga tctttgcctg gtctctgatc atcccattca gctgctttcc
     1681 taagcacctg ccaggaacag cagagatcca ggcaggcaag acctcccagg cacaccagtc
     1741 taacagcaat gccgacgtga agttcggcaa gtctatcaag gattttcccg ccgccctgaa
     1801 gaatctgatg aagaacgccg tgttcatgtg cctggtgctg agcacaagct ccgaggccct
     1861 gatcaccaca ggcttcgcca cctttctgcc caagttcatc gagaatcagt ttggcctgac
     1921 atctagcttt gccgccaccc tgggaggagc cgtgctgatc cctggagccg ccctgggcca
     1981 gatcctgggc ggcttcctgg tgtccaagtt taggatgaca tgcaagaata ccatgaagtt
     2041 cgccctgttt acatccggcg tggccctgac cctgtctttc gtgtttatgt atgccaagtg
     2101 tgagaacgag ccattcgccg gcgtgtccga gtcttacaat ggaaccggag agctgggaaa
     2161 cctgatcgca ccctgcaacg ccaattgcaa ctgtagccgc tcctactatt accccgtgtg
     2221 cggcgacggc gtgcagtact tcagcccttg ctttgcagga tgttccaatc cagtggcaca
     2281 caggaagcct aaggtgtatt acaactgctc ttgtatcgag agaaagacag agatcaccag
     2341 cacagccgag accttcggct ttgaggccaa ggccggcaag tgcgagacac actgtgccaa
     2401 gctgcctatc ttcctgtgca tcttctttat cgtgatcatc ttcaccttta tggccggcac
     2461 accaatcacc gtgagcatcc tgagatgcgt gaaccacagg cagcggagcc tggccctggg
     2521 catccagttt atggtgctga ggctgctggg aaccatccct ggaccaatca tcttcggctt
     2581 tacaatcgac agcacctgca tcctgtggga catcaatgat tgcggcatca agggcgcctg
     2641 ttggatctat gataacatca agatggccca catgctggtg gccatctccg tgacatgtaa
     2701 agtgatcacc atgttcttta atggcttcgc catctttctg tacaagccac cccctagcgc
     2761 cacagacgtg tccttccaca aggagaatgc cgtggtgacc aacgtgctgg ccgagcagga
     2821 tctgaacaag atcgtgaagg agggcgaccc agctttcttg tacaaagttg gcattataag
     2881 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2941 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     3001 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     3061 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     3121 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     3181 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     3241 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     3301 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     3361 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     3421 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     3481 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     3541 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     3601 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     3661 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     3721 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     3781 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     3841 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     3901 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     3961 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     4021 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4081 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4141 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4201 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4261 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4321 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4381 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4441 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4501 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4561 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4621 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4681 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
