LOCUS       sequence-263913-        9040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing NPM1 wildtype (human) in mammalian 
            cells upon transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Wang AJ, Han Y, Jia N, Chen P, Minden MD
  TITLE     NPM1c impedes CTCF functions through cytoplasmic mislocalization in 
            acute myeloid leukemia.
  JOURNAL   Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. 
            Epub 2019 Dec 12.
  PUBMED    31831844
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-019-0681-8"; journalName: "Leukemia"; date: 
            "2020-05"; volume: "34"; issue: "5"; pages: "1278-1290"
COMMENT     Sequence Label: pLVX-NPM1wt-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..9040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3711..4283
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3734..4285
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3878..3895)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             4285..4995
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4430..4448)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4698..4717
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5009..5597
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5062..5082)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5480..5491)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5804..6437
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6565..6581)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6565..6581)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6578..6600)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6589..6605
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6613..6619,6620..6637,6638..6643))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6658..6679
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6796..6813)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6967..7555)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7047..7066)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7726..8517,8518..8586))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8349..8368
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8587..8691)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8739..8873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8776..8795)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8830..8849
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcta tggaagattc
     2821 gatggacatg gacatgagcc ccctgaggcc ccagaactat cttttcggtt gtgaactaaa
     2881 ggccgacaaa gattatcact ttaaggtgga taatgatgaa aatgagcacc agttatcttt
     2941 aagaacggtc agtttagggg ctggtgcaaa ggatgagttg cacattgttg aagcagaggc
     3001 aatgaattac gaaggcagtc caattaaagt aacactggca actttgaaaa tgtctgtaca
     3061 gccaacggtt tcccttgggg gctttgaaat aacaccacca gtggtcttaa ggttgaagtg
     3121 tggttcaggg ccagtgcata ttagtggaca gcacttagta gctgtggagg aagatgcaga
     3181 gtcagaagat gaagaggagg aggatgtgaa actcttaagt atatctggaa agcggtctgc
     3241 ccctggaggt ggtagcaagg ttccacagaa aaaagtaaaa cttgctgctg atgaagatga
     3301 tgacgatgat gatgaagagg atgatgatga agatgatgat gatgatgatt ttgatgatga
     3361 ggaagctgaa gaaaaagcgc cagtgaagaa atctatacga gatactccag ccaaaaatgc
     3421 acaaaagtca aatcagaatg gaaaagactc aaaaccatca tcaacaccaa gatcaaaagg
     3481 acaagaatcc ttcaagaaac aggaaaaaac tcctaaaaca ccaaaaggac ctagttctgt
     3541 agaagacatt aaagcaaaaa tgcaagcaag tatagaaaaa ggtggttctc ttcccaaagt
     3601 ggaagccaaa ttcatcaatt atgtgaagaa ttgcttccgg atgactgacc aagaggctat
     3661 tcaagatctc tggcagtgga ggaagtctct ttaagaaaat agggatcccg cccctctccc
     3721 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     3781 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     3841 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     3901 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     3961 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     4021 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     4081 tggatagttg tggaaagagt caaatggctc acctcaagcg tattcaacaa ggggctgaag
     4141 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     4201 acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     4261 tcctttgaaa aacacgatga taatatggtg agcaagggcg aggaggataa catggccatc
     4321 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     4381 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     4441 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     4501 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     4561 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     4621 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     4681 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     4741 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     4801 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     4861 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     4921 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     4981 gagctgtaca agtgaacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag
     5041 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5101 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5161 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5221 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5281 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5341 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5401 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5461 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5521 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5581 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca
     5641 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     5701 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     5761 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac
     5821 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     5881 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     5941 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     6001 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     6061 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     6121 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     6181 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     6241 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6301 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6361 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6421 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     6481 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc
     6541 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6601 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6661 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6721 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6781 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6841 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6901 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6961 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7021 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7081 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7141 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7201 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7261 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7321 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7381 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7441 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7501 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7561 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7621 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7681 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7741 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7801 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7861 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7921 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7981 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8041 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8101 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8161 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8221 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8281 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8341 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8401 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8461 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8521 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8581 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8641 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8701 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata
     8761 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8821 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     8881 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     8941 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
     9001 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
