LOCUS       sequence-263927-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr24
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(612..630)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(613..630)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(648..664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(648..664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(661..683)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    672..688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(696..702,703..720,721..726))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    741..762
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(879..896)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccgacgtcg catgctcccg gccgccatgg cggccgcggg aattcgattc aaaaccacac
       61 cagtgcatct gaattcatcc tcttgggact ttcagaaaag ccagatcatg accctgtcct
      121 cttttctctg ttcctctgca tgtacatgat cacagtggtg gggaacttgt tgatcattct
      181 tgccatcagc tttgactctc acctccatac ccctatgtat ttcttcctgg ccaatctctc
      241 cttggttgat ttctgtctgg ctaccaacac tgtccccaag atgctggtga atattcaaac
      301 taggaacaaa tctatctctt atccttgttg tctgacccag atgtactttt tccatttttt
      361 tggcatcatg gacagtgtct taatagctgt gatggcttac gaccggtttg tggccatttg
      421 tcacccatta cattattcta ctatcatgag tccacggctc tgtggcctgc tggttggtgt
      481 cccatgggta tattcctgct tcatctccct cactcacatc ctcctcatgg caatcactag
      541 tgaattcgcg gccgcctgca ggtcgaccat atgggagagc tcccaacgcg ttggatgcat
      601 agcttgagta ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt
      661 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      721 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      781 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      841 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
      901 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      961 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1021 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccn tgacgagcat
     1081 cccaagat
//
