LOCUS       sequence-263929-        1246 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr16
FEATURES             Location/Qualifiers
     source          1..1246
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1016..1034)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1017..1034)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1052..1068)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1052..1068)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1065..1087)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1076..1092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1100..1106,1107..1124,1125..1130))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 cnnttgggcc cgacgtcgca tgctcccggc cgccatggcc gcgggattcc tgaagtgata
       61 gagttcgtct tcctgggatt ctccagcttt ggaaagcatc agataaccct ctttgtggtt
      121 ttcctaacca tctacatttt aactctggct ggcaacatca ttatagtgac aatcacacac
      181 atagaccacc accttcacac tcccatgtac ttctttctga gcatgttggc aagctcagag
      241 actgtgtaca cactggtcat tgtcccacga atgctttcca gcctgatttt ttacaacctt
      301 cccatatcct tggcaggctg cgcaacccaa atgttctttt ttgtcacttt ggccaccaac
      361 aactgctttc tgctcacagc aatgggttat gatcgttatg tggctatttg taatcctctg
      421 agatatacaa tcatcatgag caagggaatg tgtgccttgt tggtctgtgg gtctttaggc
      481 actggcctgg ttatggcagt tcttcatgtg ccagccatgt tccatttgcc cttttgtggc
      541 acggtggtgg agcacttttt ctgtgacata tacccagtaa tgaagctttc ttgtgttgat
      601 accaccgtca atgagataat caattatggt gtaagttcat ttgtaattct tgtgcccata
      661 gggctgatat ttatctccta tgtgctcatt gtctcttcca tccttaaaat tgtgtccact
      721 gaaggccaga agaaagcctt tgccacctgt gcctctcatc tcactgtggt cattgtccac
      781 tatggctgtg cctccattgc ctacctcaaa cccaaatcag aaagttcagt agaaaaagac
      841 cttcttctct ctgtgaccta cactatcatc actcccttgc tgaaccctgt tgtctacagc
      901 ctcaggaaca aagaagtcaa agatgctcta tgcagagctg taatcactag tgcggccgcc
      961 tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat
     1021 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1081 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1141 cctaatgagt gagctaactc mcattaattg cgtngcgctc actgcccgct ttccagtcgg
     1201 gaaacctgtc nngccagctg cattaatgaa tcggccaacs ccccgg
//
