LOCUS       sequence-263933-        1230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr77.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr77
FEATURES             Location/Qualifiers
     source          1..1230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(633..651)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(634..651)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(669..685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(682..704)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    693..709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(717..723,724..741,742..747))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    762..783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(900..917)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1151..1170)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaattgggs ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga attcgattgc
       61 cccctttaca tccttgtttc gcaagctata gatgaagggg ttcaacatag gggtgacaac
      121 agtgtacatt attgaagtga ttgtgtttct cttggatgaa tgggctacag cagaactgag
      181 atgaactaca aatcctgtcc catagaacat ggataccaca cagaggtgag acccacaggt
      241 ggaaaaggct ttatacttat tcttaatgga ggacattttc attaaagcag agacaatgtg
      301 aaaataagag aaagctatcc caatgacagg aaacatacct agcactgcag taaccaaata
      361 cagaaaaatg ttatttatgt gggtatcaga gcgggccacc ctgagaagct cagtgagttc
      421 acaaaagaaa tgtgggattt cagtgctagt agagaagttc agttgtttca tcaacagcat
      481 atgaatcaag gaaacccaga acataatgat ccaagataaa agaaccagaa ggccacagat
      541 tcgagggttc ataatcacta gtgaattcgc ggccgcctgc aggtcgacca tatgggagag
      601 ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa tagcttggcg
      661 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      721 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      781 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      841 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      901 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      961 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1021 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1081 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1141 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgss ctyycctgtt
     1201 ccgaccctgc cscttaccgg awacctgttc
//
