LOCUS       sequence-263959-        1234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1402.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1402
FEATURES             Location/Qualifiers
     source          1..1234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(814..832)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(815..832)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(850..866)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(850..866)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(863..885)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    874..890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(898..904,905..922,923..928))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    943..964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1081..1098)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgattgggcc cgacgtcgca tgctcccggc cgccatggcg gccgcgggaa ttcgattggc
       61 tgttccaaag aaaaccacca ccacagtgag gtgtggggca caggtggaga aggcctttct
      121 cttgccctga gtagagggag tcctcaggat ggcagagatg atcagtatat aggagcctgc
      181 agtgaacagg cagggactca ggccaacagt ggcactggac acatggagga cagattcatt
      241 gacagaggtg tctgagcagg agagcttcag gagcaatggg acatcacaga ggaagtggct
      301 gatttggttg gggccacaca gagtaagtgt gcttgtcaat actgtgtgag tcacagaatt
      361 caccaagcca cagagccagg atccagtcac caagctgata cagacccttg tgctcatgag
      421 aactggatac cgaagagggt gacaaacggc cacatagcgg tcatatgcca tggcagccaa
      481 caagacacat tcagttccag cacatgacac aaagaagaag atctgagaga ggcagccctc
      541 aaaggagatg gtcttcttct ccctgaagaa gttcactagc atgactggaa tggaggtaga
      601 tgtgtagcag atgtccagga agctcaagtt gctgaggaaa tagtacattg gagtgtggag
      661 tgcagggctg accctggtgg ccactatcat gagtgtgttc cccaggagag ttgtcaggta
      721 aatggccagg aagaatcact agtgaattcg cggccgcctg caggtcgacc atatgggaga
      781 gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa atagcttggc
      841 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      901 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      961 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1021 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1081 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1141 aaaggcggta atacggttat ccmmraatca ggggataacg caggaaagaa ctgtgagcaa
     1201 aggccagcaa aggccaggaa ccgtaaaaag gccs
//
