LOCUS       sequence-263960-        1200 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1412.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1412
FEATURES             Location/Qualifiers
     source          1..1200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(629..640)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(739..757)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(740..757)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(775..791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(775..791)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(788..810)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    799..815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(823..829,830..847,848..853))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    868..889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1006..1023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agggcgattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tataaagcag aaggcccagg tccaagcttg ggctctcaat aggctaggct tagccaccta
      121 tatgctactt atagagtcaa gagatggctt gagtcaaaga gaggaagttt aattaatata
      181 aatacattgt tcagagtaaa tagcaaggtc cagtgagtga cctgagtcca ttagtggggg
      241 tactgatgag aattttatgt tgaaatggga cagggattgg gacagaggac aagtgatgtg
      301 gtgtggttgg tcatgctcat cacagagggt ctccatcata gttctagaga acagcccaag
      361 aaaccctctg ctgtttgaag gaaaaattgg ttctcaggag gtaattattt cgcctccaag
      421 gagcagcctc atccttgtag ataattgtcc taatacaggg gtctatcctg agagactatg
      481 ctgctcctgg catacaagag gatggaagaa atcacacata aatgccagaa ggaggaaagt
      541 atgcaggaag ttacccagaa gcaaacaaga aaacaagatt caaaccacat ataaactgcc
      601 ctagtgatga gattgtcttc cacacagccc ttccttctat ctccaggatg ccttccttaa
      661 tcactagtga attcgcggcc gcctgcaggt cgaccatatg ggagagctcc caacgcgttg
      721 gatgcatagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata
      781 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      841 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      901 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      961 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cawaggctcc gccccctgac
//
