LOCUS       sequence-263961-        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1440.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1440
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(672..690)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(673..690)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(708..724)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(708..724)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(721..743)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    732..748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(756..762,763..780,781..786))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    801..822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(939..956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgattgggcc cgacgtcgca tgctcccggc cgccatggcg gccgcgggaa ttcgattgac
       61 ttgcagggga aaggtttcag agcaggaaag aactaatagc tgaggcaggt cacaaaagaa
      121 atggtttata atatttggcc cacagaaatg gagctgaagt atagcacaca gttgaatcaa
      181 tgaacccaag agaccagtac tataggctcc aaccaccatg tgcacacaga gtgacgggga
      241 catgatggct gtgtagagaa gaggattaca aatggcagca taccggtcat aagccatggc
      301 tgccagaagg cagcattcgg acagacccag gcttgagaag atgaagtact gcatggtgca
      361 cccaacaaag gagatagatt tagacttctg gaagaatccc gagagcattt taggggttgt
      421 agatgaaatg taacagaaat ctaaaaatga caaattgctg aggaagaagt acataggtgt
      481 gtgtaggtaa gggtcaatcc taatcaagat gatgaggccc aagttccaca ccactgtgga
      541 gagataactt cccaggaaga taacaaagag aaccagcttg agctttggaa aaatcactag
      601 tgaattcgcg gccgcctgca ggtcgaccat atgggagagc tcccaacgcg ttggatgcat
      661 agcttgagta ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt
      721 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      781 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      841 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      901 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
      961 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1021 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1081 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1141 cmcaaaaatc gacgctcnag tcagaggngg cnaaacccga caggactwta agawnccagg
     1201 cgtttccccc tggaagctcc ctcgkgssc
//
