LOCUS       sequence-263966-        1171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1415.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1415
FEATURES             Location/Qualifiers
     source          1..1171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(710..728)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(711..728)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(746..762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(746..762)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(759..781)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    770..786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(794..800,801..818,819..824))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    839..860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(977..994)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgattgggs ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga attcgattac
       61 gcacattcct gtgtttcatc aggttcctgt ttctagccca gatacagttc tgtgtagttt
      121 actaactgca tgggtgtcag taaaatgaaa agaaaatact acctggattt tatatagcag
      181 catttacaca taccagacat tatccataat actgtcagca catggcgaag cacagaactg
      241 catgctgata catgcatgaa tgctactgag caagcacaca tgaatcattg ctacatgcaa
      301 agacagggag aaccaagcac atggaactgg gcataatcag aacatgaaag tgtaagtact
      361 taatgaccat agtcacaaag ccttccaaac acccaggtta ccccctgaca ttaggggtaa
      421 ccaacacagt gggtctcagg gaacagcgag tgtgactaga atctacctag gagctaggta
      481 cccaagacta cacatattgt atataacaat gtcagaaaac atatttggat gaagactcat
      541 tatgtagctg gctctgtaat agcactagat tttaccaaaa gaattgtaaa gaacattagg
      601 tactatacaa gagacttttt ccaccacaaa atcactagtg aattcgcggc cgcctgcagg
      661 tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc
      721 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      781 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      841 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      901 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      961 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1021 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1081 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1141 tggcgttttt cntaggctcc gccccctgac g
//
