LOCUS       sequence-263967-        1185 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr167.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr167
FEATURES             Location/Qualifiers
     source          1..1185
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(679..697)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(680..697)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(715..731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(715..731)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(728..750)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    739..755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(763..769,770..787,788..793))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    808..829
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(946..963)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgattggg cccgacgtcg catgctcccg gccgccatgg cggccgcggg aattcgattt
       61 gatcctcctc atcctcctgg attcacatct ccacacaccc atgtattttt tacttagtca
      121 gctttctctt attgacttaa attacatctc taccattgtc cctaaaatgg tatctgactt
      181 tatgttaggt aataaataca tctccttcat tgggtgtgga tttcagattt tcctcttctt
      241 gacttttggg ggtgcagaaa cccttttgtt ggcatctatg gcctatgacc gttatgtggc
      301 tatttgtttt cctctccact atgccacaca tatgaataaa agagtctgtg tgatgatgat
      361 aacaggagct tggattctgg gctccattaa ttcctgtgct catactggat atgcactcca
      421 aattccttac tgcagatcca gagccatcaa ccatttcttc tgtgatgtcc ctgccatgtt
      481 gactctggct tgcacagaca catgggtcta tgagtataca gtgtttgtga gtacaatcct
      541 ctttcttgtg tttccattca ttggaatagt gtgttcctat ggtcgtgttt tccttgctaa
      601 tcactagtga attcgcggcc gcctgcaggt cgaccatatg ggagagctcc caacgcgttg
      661 gatgcatagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata
      721 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      781 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      841 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      901 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      961 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1021 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1081 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gccccctgac
     1141 gagcatcmca aaatcgacgc tcaagtcaga ggtggcgaaa cccga
//
