LOCUS       sequence-263968-        1158 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr533.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr533
FEATURES             Location/Qualifiers
     source          1..1158
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(600..618)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(601..618)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(636..652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(636..652)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(649..671)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    660..676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(684..690,691..708,709..714))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    729..750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(867..884)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1118..1137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agggcgattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tggatcacaa aaaggatttc actcaatatt tgacaagatt tgatatgttt agtcatagaa
      121 atatcttttt tattagatat cttctttatt tacatttcaa atgttatccc ctttcctagt
      181 ttcctctcca aaaatcacct atcccctccc cccacccctg atccccaacc cacccactcc
      241 tgcttcctgg ccccggcatt cgcctatact ggggcataga atcttcccaa gaccaaaaaa
      301 gaaatatctt aacatttaat agtactgaac ttataagcac acatttccaa gtatgaagta
      361 gaaattagca aagctatcta aaacattcaa aagagaagaa ctctgttatt atcttgtaga
      421 tagtatgaca tagacactac aaactttgga gaattaagcc aggctataac attgcagaac
      481 ttggttgact tttttagctc attacccatt gcttttagaa atcactagtg aattcgcggc
      541 cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt
      601 ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      661 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      721 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      781 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      841 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      901 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      961 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1021 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1081 cgctcaagtc agaggnggcg aaacccgaca ggactwtaaa gataccaggc gtttccccct
     1141 ggaagctccc tcgtgsgc
//
