LOCUS       sequence-263969-        1198 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr536.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr536
FEATURES             Location/Qualifiers
     source          1..1198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(693..711)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(694..711)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(729..745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(729..745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(742..764)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    753..769
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(777..783,784..801,802..807))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    822..843
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(960..977)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgattgggcc cgacgtcgca tgctcccggc cgccatggcg gccgcgggaa ttcgattaga
       61 gatcctaggc ccccataccc agccctaact cagtttgttt attagtttcc ttccttcaac
      121 tattaaatga tccaagcacc agtgtaaact gtgagataag aataagaaag atgaaaacag
      181 ccaaatcagg ccagcaaatg ctcagagcag atgtgtgtat ggcgggggtg ggagcagagg
      241 gagtcatgtt tctttatctc tataagcatt cacttgcagc cagtgacaca gaggatcagg
      301 gaattttaaa taggtcatta aaaggactat actcaatatt taactacatt tggttcagtc
      361 atagaagcat cttaacattt aagagcattg aacttataag cacacacatc tatgtatgaa
      421 gtagaaatgg ctaaagctta ctaaaacatc ttgtagacag catgattttc tgtatacaga
      481 ctttggacag ctgtcccagg ctataactta gcctgtgccc tccggagtca gatgttacag
      541 aaaggaaggt ttcagtgatc ccttgagctc cttgccagct cttctccaag ctcaaagtca
      601 tcaacgggga aaaatcacta gtgaattcgc ggccgcctgc aggtcgacca tatgggagag
      661 ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa tagcttggcg
      721 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      781 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      841 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      901 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      961 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1021 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1081 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1141 ctccgccccc tgacgagcat cmmaaaatcg angctnaagt caaaggggcg aaacccga
//
