LOCUS       sequence-263974-        1159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1509
FEATURES             Location/Qualifiers
     source          1..1159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(602..620)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(603..620)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(638..654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(638..654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(651..673)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    662..678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(686..692,693..710,711..716))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    731..752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(869..886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgattgggc ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga attcgatttg
       61 ggccacagta gggtagacga atagtcaaga aggtctgcac aagtgaatga atagtgcccc
      121 ccagccagag tgcaaagaca agctgtacac agaccttcat gttcatcaca ttggagtaat
      181 gcaatgggat gcagatagcc acataacgat catacgccat aatcgtcagc agaaagatct
      241 cagaacaagc aaagagatgt aggaagaaga gctgtgcaat gcaattgtca aaggaaatgg
      301 tcttcctccc taaaagcaaa ccctcaagca tcttgggcac ggtgacagat gagtggcaga
      361 tgtcaatgaa ggacagattg ctcagaaaga agtacatggg attgtggaga cgtggcgtga
      421 agactatggt aacaacaatg aggaagttcc ccaaaagtgt taacatataa gtgactgtaa
      481 atggtacgaa aaacagaatc tccaacaccc agttgtcagt gaatcactag tgaattcgcg
      541 gccgcctgca ggtcgaccat atgggagagc tcccaacgcg ttggatgcat agcttgagta
      601 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      661 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      721 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      781 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      841 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagannccag gcgtttcccc
     1141 tggaagatcc ctcgtcsct
//
