LOCUS       sequence-263980-        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr157.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr157
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(879..897)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(880..897)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(915..931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(915..931)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(928..950)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    939..955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(963..969,970..987,988..993))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1008..1029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agggcgattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tggaagccag cacaagcatt ggctgcaaag ggcacacact ggtaacacac acatggttca
      121 cacacctgtg ctgacgtggt tgttttgtgt caaaggccgg tttcccctga agctgactct
      181 tgtggagaga tttgcatgca ggatgtttgc tgtggcaact gttaaaggga tcaggcccga
      241 gcaggggaca acatgaactg ccacagagaa gcacttgtgg cgaagctttt agagataaca
      301 aagccttctc attaccaatg atggtgacag gatgagggct gatgtaagaa gagagtgtgg
      361 cactgggtga ggtggctctc cttagtaagg agcagtgctg cagagagatc cagatgttgg
      421 cagtcactga caggtccagt ggctggggtg ataagtattt ctggggatcc agacaataca
      481 ccagggcatc cattccatgg tcttaactga tacttctcca ggtccagcat gcatataaat
      541 ccagtggagg atcttgttaa aatgaacttt cccagtcagt agatctgctg ggggtcagga
      601 aactctgtat gccaaaaagc tcccgggtga tgggtgatgt tgacatttcc agtctgtgga
      661 tcacactagg aatgactcta agacagcttg ttcctcacct agcattgcct tgcaataaga
      721 ttctcaagtg agagccatca gaacatgtgg atcatgggct ctatccctcc attagaaggt
      781 gaggcatctg tgacttgcaa tcactagtga attcgcggcc gcctgcaggt cgaccatatg
      841 ggagagctcc caacgcgttg gatgcatagc ttgagtattc tatagtgtca cctaaatagc
      901 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      961 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     1021 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     1081 ctgcattaat gaatcggcca acgcgcgggg aagaggcggt ttgcgtattg g
//
